cDNA Clone UHRF1

• Synonyms: ICBP90, Np95, RNF106, hNP95, hUHRF1, huNp95
• Plasmid: pEX UHRF1 C-GST
• Parent Plasmid: pEX C-GST
• Expressed: UHRF1-GST
• Genbank: NM_001048201.2
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCGCCATGTGGATCCAGGTTCGGACCATGGATGGGAGGCAGACCCACACGGTGGACTCGCTGTCCAGGCTGACCAAGGTGGAGGAGCTGAGGCGGAAGATCCAGGAGCTGTTCCACGTGGAGCCAGGCCTGCAGAGGCTGTTCTACAGGGGCAAACAGATGGAGGACGGCCATACCCTCTTCGACTACGAGGTCCGCCTGAATGACACCATCCAGCTCCTGGTCCGCCAGAGCCTCGTGCTCCCCCACAGCACCAAGGAGCGGGACTCCGAGCTCTCCGACACCGACTCCGGCTGCTGCCTGGGCCAGAGTGAGTCAGACAAGTCCTCCACCCACGGTGAGGCGGCAGCCGAGACTGACAGCAGGCCAGCCGATGAGGACATGTGGGATGAGACGGAATTGGGGCTGTACAAGGTCAATGAGTACGTCGATGCTCGGGACACGAACATGGGGGCGTGGTTTGAGGCGCAGGTGGTCAGGGTGACGCGGAAGGCCCCCTCCCGGGACGAGCCCTGCAGCTCCACGTCCAGGCCGGCGCTGGAGGAGGACGTCATTTACCACGTGAAATACGACGACTACCCGGAGAACGGCGTGGTCCAGATGAACTCCAGGGACGTCCGAGCGCGCGCCCGCACCATCATCAAGTGGCAGGACCTGGAGGTGGGCCAGGTGGTCATGCTCAACTACAACCCCGACAACCCCAAGGAGCGGGGCTTCTGGTACGACGCGGAGATCTCCAGGAAGCGCGAGACCAGGACGGCGCGGGAACTCTACGCCAACGTGGTGCTGGGGGATGATTCTCTGAACGACTGTCGGATCATCTTCGTGGACGAAGTCTTCAAGATTGAGCGGCCGGGTGAAGGGAGCCCCATGGTTGACAACCCCATGAGACGGAAGAGCGGGCCGTCCTGCAAGCACTGCAAGGACGACGTGAACAGACTCTGCCGGGTCTGCGCCTGCCACCTGTGCGGGGGCCGGCAGGACCCCGACAAGCAGCTCATGTGCGATGAGTGCGACATGGCCTTCCACATCTACTGCCTGGACCCGCCCCTCAGCAGTGTTCCCAGCGAGGACGAGTGGTACTGCCCTGAGTGCCGGAATGATGCCAGCGAGGTGGTACTGGCGGGAGAGCGGCTGAGAGAGAGCAAGAAGAAGGCGAAGATGGCCTCGGCCACATCGTCCTCACAGCGGGACTGGGGCAAGGGCATGGCCTGTGTGGGCCGCACCAAGGAATGTACCATCGTCCCGTCCAACCACTACGGACCCATCCCGGGGATCCCCGTGGGCACCATGTGGCGGTTCCGAGTCCAGGTCAGCGAGTCGGGTGTCCATCGGCCCCACGTGGCTGGCATACACGGCCGGAGCAACGACGGAGCGTACTCCCTAGTCCTGGCGGGGGGCTATGAGGATGACGTGGACCATGGGAATTTTTTCACATACACGGGTAGTGGTGGTCGAGATCTTTCCGGCAACAAGAGGACCGCGGAACAGTCTTGTGATCAGAAACTCACCAACACCAACAGGGCGCTGGCTCTCAACTGCTTTGCTCCCATCAATGACCAAGAAGGGGCCGAGGCCAAGGACTGGCGGTCGGGGAAGCCGGTCAGGGTGGTGCGCAATGTCAAGGGTGGCAAGAATAGCAAGTACGCCCCCGCTGAGGGCAACCGCTATGATGGCATCTACAAGGTTGTGAAATACTGGCCCGAGAAGGGGAAGTCCGGGTTTCTCGTGTGGCGCTACCTTCTGCGGAGGGACGATGATGAGCCTGGCCCTTGGACGAAGGAGGGGAAGGACCGGATCAAGAAGCTGGGGCTGACCATGCAGTATCCAGAAGGCTACCTGGAAGCCCTGGCCAACCGAGAGCGAGAGAAGGAGAACAGCAAGAGGGAGGAGGAGGAGCAGCAGGAGGGGGGCTTCGCGTCCCCCAGGACGGGCAAGGGCAAGTGGAAGCGGAAGTCGGCAGGAGGTGGCCCGAGCAGGGCCGGGTCCCCGCGCCGGACATCCAAGAAAACCAAGGTGGAGCCCTACAGTCTCACGGCCCAGCAGAGCAGCCTCATCAGAGAGGACAAGAGCAACGCCAAGCTGTGGAATGAGGTCCTGGCGTCACTCAAGGACCGGCCGGCGAGCGGCAGCCCGTTCCAGTTGTTCCTGAGTAAAGTGGAGGAGACGTTCCAGTGTATCTGCTGTCAGGAGCTGGTGTTCCGGCCCATCACGACCGTGTGCCAGCACAACGTGTGCAAGGACTGCCTGGACAGATCCTTTCGGGCACAGGTGTTCAGCTGCCCTGCCTGCCGCTACGACCTGGGCCGCAGCTATGCCATGCAGGTGAACCAGCCTCTGCAGACCGTCCTCAACCAGCTCTTCCCCGGCTACGGCAATGGCCGGGCGGCCGC

Amino Acid Sequence

MWIQVRTMDGRQTHTVDSLSRLTKVEELRRKIQELFHVEPGLQRLFYRGK
QMEDGHTLFDYEVRLNDTIQLLVRQSLVLPHSTKERDSELSDTDSGCCLG
QSESDKSSTHGEAAAETDSRPADEDMWDETELGLYKVNEYVDARDTNMGA
WFEAQVVRVTRKAPSRDEPCSSTSRPALEEDVIYHVKYDDYPENGVVQMN
SRDVRARARTIIKWQDLEVGQVVMLNYNPDNPKERGFWYDAEISRKRETR
TARELYANVVLGDDSLNDCRIIFVDEVFKIERPGEGSPMVDNPMRRKSGP
SCKHCKDDVNRLCRVCACHLCGGRQDPDKQLMCDECDMAFHIYCLDPPLS
SVPSEDEWYCPECRNDASEVVLAGERLRESKKKAKMASATSSSQRDWGKG
MACVGRTKECTIVPSNHYGPIPGIPVGTMWRFRVQVSESGVHRPHVAGIH
GRSNDGAYSLVLAGGYEDDVDHGNFFTYTGSGGRDLSGNKRTAEQSCDQK
LTNTNRALALNCFAPINDQEGAEAKDWRSGKPVRVVRNVKGGKNSKYAPA
EGNRYDGIYKVVKYWPEKGKSGFLVWRYLLRRDDDEPGPWTKEGKDRIKK
LGLTMQYPEGYLEALANREREKENSKREEEEQQEGGFASPRTGKGKWKRK
SAGGGPSRAGSPRRTSKKTKVEPYSLTAQQSSLIREDKSNAKLWNEVLAS
LKDRPASGSPFQLFLSKVEETFQCICCQELVFRPITTVCQHNVCKDCLDR
SFRAQVFSCPACRYDLGRSYAMQVNQPLQTVLNQLFPGYGNGRAAALEVL
FQGPLSRMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRN
KKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEIS
MLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYL
NGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYL
KSSKYIAWPLQGWQATFGGGDHPPKSDL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU48461
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 4 silent mutations as compared to the reference sequence; C>T 27 bp, C>A 342 bp, T>C 1407 bp and C>T 1665 bp.; ; Estimated MW of expressed protein : 117689.58 Daltons