Synonyms
KIAA0564
Plasmid
pcDNA5D FRT/TO GFP-VWA8-HA
Parent Plasmid
pcDNA5D FRT/TO GFP
Expressed
GFP-VWA8-HA
Genbank
NM_015058.1 GI:57863270 with silent changes t1941c and t3642c
Tag
HA
GFP
Species
Human
Sequence of Insert
View Sequence
GGATCCGCCACCATGCAATCCCGGCTTCTACTCCTCGGGGCACCCGGAGGCCACGGCGGCCCGGCCTCGCGGCGCATGCGGCTGCTCCTGCGGCAGGTGGTGCAGCGCAGGCCGGGTGGCGACAGGCAGCGGCCGGAGGTCAGACTGTTGCACGCCGGCTCGGGGGCCGACACAGGTGATACAGTTAATATTGGAGATGTATCCTACAAGTTGAAAATTCCTAAGAATCCAGAACTTGTGCCACAGAACTACATTTCAGACTCTCTGGCTCAATCTGTAGTTCAGCATCTAAGATGGATAATGCAGAAGGATCTTTTGGGGCAAGATGTTTTTCTAATAGGACCTCCTGGGCCTCTTCGACGCTCTATTGCTATGCAGTACTTGGAGCTGACCAAACGGGAGGTCGAATACATTGCCCTGTCAAGGGACACCACTGAAACTGATCTCAAACAGCGACGAGAGATCCGTGCAGGCACAGCCTTTTACATTGATCAGTGTGCAGTTCGTGCAGCCACAGAAGGCAGAACTCTCATTTTGGAAGGTTTGGAAAAGGCAGAGAGGAATGTTTTGCCTGTTTTGAACAACTTGCTGGAAAACAGAGAGATGCAGCTTGAAGATGGACGCTTCCTGATGTCTGCTGAGCGTTACGACAAACTTCTCCGAGATCATACCAAAAAAGAGTTGGATTCTTGGAAAATTGTCCGAGTTAGTGAAAATTTCCGAGTGATTGCCTTGGGCTTGCCAGTGCCAAGGTATTCTGGGAATCCATTAGACCCCCCTCTTCGTTCTCGATTTCAAGCCAGGGATATTTATTATTTACCCTTCAAGGACCAACTTAAGTTGTTATATTCAATTGGAGCCAATGTTTCTGCTGAGAAAGTTTCTCAGCTCTTGTCCTTTGCCACAACTCTGTGTTCCCAAGAATCTTCTACTCTTGGACTTCCAGACTTTCCTTTAGATAGTTTAGCAGCTGCGGTTCAAATCTTGGATTCCTTTCCTATGATGCCAATCAAACATGCAATCCAGTGGCTTTATCCATATAGTATTTTACTAGGTCATGAAGGGAAGATGGCTGTGGAAGGTGTTTTAAAGCGCTTTGAACTTCAAGATTCAGGAAGCTCTCTACTTCCTAAAGAGATTGTAAAAGTAGAGAAGATGATGGAAAACCATGTGTCCCAAGCTTCTGTGACCATCCGGATTGCAGATAAAGAGGTGACCATTAAGGTGCCAGCCGGGACCAGGCTATTAAGTCAACCTTGTGCGTCAGACCGTTTCATACAGACTTTGAGCCATAAGCAGCTACAGGCTGAAATGATGCAGTCTCACATGGTTAAAGATATATGTTTAATTGGAGGAAAGGGTTGTGGAAAAACAGTGATCGCTAAGAACTTTGCCGATACCTTAGGATACAACATAGAACCTATTATGCTCTATCAGGATATGACAGCGCGTGATCTGCTACAGCAGAGATACACCCTTCCAAATGGAGACACTGCCTGGCGGTCCTCACCCCTTGTGAATGCTGCTCTGGAAGGCAAGCTGGTCCTGCTGGATGGCATTCACCGGGTGAATGCGGGCACGCTTGCTGTATTGCAAAGGTTAATCCATGATCGAGAGCTAAGCCTCTATGATGGTTCTAGGCTGCTGAGAGAAGACAGGTATATGCGTTTAAAGGAGGAGCTGCAACTGTCTGATGAACAGCTACAGAAGAGATCCATTTTTCCTATCCATCCCTCCTTCAGAATCATTGCCTTGGCAGAACCCCCTGTTATTGGAAGCACAGCACACCAGTGGCTGGGACCAGAATTCTTAACCATGTTCTTTTTCCATTACATGAAACCACTTGTGAAAAGTGAAGAAATCCAAGTGATTAAGGAAAAGGTCCCAAATGTACCTCAGGAAGCTCTGGATAAGTTATTATCATTTACACACAAACTCAGAGAAACACAGGACCCAACGGCACAATCATTAGCGGCATCACTTTCTACCAGACAACTGTTGCGAATTTCTCGTCGGCTGTCACAGTATCCTAATGAAAATCTTCACAGTGCTGTTACTAAAGCCTGCCTTTCCAGGTTTTTACCCAGTCTTGCTAGGTCAGCATTAGAAAAAAATCTGGCAGATGCTACAATAGAAATAAATACTGATGACAATTTGGAGCCAGAACTGAAGGATTACAAATGTGAAGTAACATCTGGAACTCTGAGGATTGGTGCTGTTAGTGCACCGATCTATAATGCACATGAGAAAATGAAAGTGCCTGATGTTCTTTTCTATGACAACATTCAGCATGTGATAGTGATGGAAGATATGCTGAAAGACTTTCTCCTTGGAGAACACTTATTATTGGTTGGCAACCAGGGTGTAGGAAAAAACAAGATTGTTGACAGATTCCTTCACCTGCTCAACAGACCCCGAGAATATATTCAGCTACACAGGGATACCACAGTACAAACTCTTACGCTTCAGCCTTCGGTTAAAGACGGACTTATTGTATATGAAGACTCACCTTTGGTTAAAGCAGTAAAGTTGGGTCATATTCTGGTAGTAGATGAGGCTGACAAAGCTCCAACAAATGTCACGTGTATTTTAAAAACTCTAGTAGAAAATGGAGAAATGATTCTAGCAGATGGAAGACGCATTGTTGCAAATTCTGCTAATGTGAATGGAAGAGAAAATGTTGTAGTGATTCATCCTGATTTTAGGATGATTGTTCTGGCAAATAGACCTGGATTTCCTTTCCTAGGCAATGATTTCTTCGGTACCTTAGGTGATATTTTTAGCTGCCATGCAGTTGATAACCCCAAACCCCACTCGGAGCTCGAGATGCTCAGACAGTATGGACCAAATGTGCCTGAGCCCATCCTTCAGAAGCTTGTGGCTGCCTTTGGAGAGCTGAGGAGTTTGGCTGACCAAGGGATTATTAACTATCCTTATTCTACCAGAGAAGTTGTCAACATAGTCAAACATTTACAGAAATTTCCGACTGAAGGTCTCTCCAGTGTAGTTCGAAATGTGTTTGACTTTGATTCCTACAACAATGACATGAGGGAGATATTGATTAACACTTTACACAAATACGGGATACCTATCGGAGCAAAGCCTACCAGTGTGCAGCTGGCAAAGGAGTTGACTCTGCCAGAACAAACGTTCATGGGCTACTGGACAATTGGTCAGGCAAGAAGTGGGATGCAAAAACTCTTGTGTCCAGTGGAAACTCATCATATAGACATAAAGGGTCCAGCACTTATAAATATACAGGAGTATCCAATAGAAAGACATGAAGAAAGATCCCTAAACTTTACTGAAGAATGTGCCTCATGGAGAATACCATTGGATGAAATTAATATAATCTGTGACATTGCTACATCACATGAAAATGAGCAAAATACTCTCTATGTAGTTACATGCAATCCCGCTTCCCTGTACTTTATGAATATGACTGGGAAAAGTGGCTTCTTTGTGGACTTTTTTGATATCTTCCCAAGAACAGCCAATGGCGTTTGGCACCCTTTTGTGACAGTGGCACCGCTGGGAAGTCCTCTCAAAGGTCAAGTGGTTCTCCATGAGCAGCAGAGTAATGTTATCCTGTTGTTAGATACTACTGGCCGGGCCCTTCATCGTCTCATCCTCCCTTCCGAGAAGTTTACATCCAAGAAACCTTTCTGGTGGAACAAAGAAGAAGCTGAAACTTATAAAATGTGTAAAGAATTTTCACACAAAAACTGGCTGGTGTTCTACAAAGAAAAAGGGAACAGCCTGACTGTGCTGGATGTTCTAGAAGGGCGAACTCACACCATCTCACTTCCCATCAACCTCAAGACAGTTTTCCTTGTAGCAGAGGACAAATGGCTTCTGGTGGAGAGCAAAACAAATCAGAAATATCTTTTAACTAAGCCTGCACACATCGAATCTGAGGGTAGTGGGGTTTGCCAGTTGTATGTGCTGAAAGAGGAGCCGCCCAGCACAGGGTTTGGAGTTACACAAGAAACAGAGTTCAGCATACCTCATAAAATTTCCAGTGATCAACTATCATCTGAACATCTAAGTTCAGCTGTGGAACAAAAGATTGCCTCTCCCAACAGAATTCTCTCAGATGAGAAAAATTATGCTACAATAGTTGTTGGTTTTCCAGATCTCATGTCACCCAGTGAAGTTTATTCTTGGAAGAGACCATCATCTTTGCATAAACGAAGTGGCACTGATACATCATTCTATAGAGGAAAGAAGAAAAGGGGGACTCCAAAACAAAGCAATTGTGTGACTCTTTTAGATACGAATCAGGTAGTGAGGATTTTACCCCCAGGAGAAGTCCCTCTAAAAGATATCTACCCAAAAGATGTTACTCCTCCACAAACATCTGGTTATATAGAAGTCACTGATCTTCAATCAAAGAAACTCCGATATATCCCTATTCCCAGATCAGAATCTCTCTCGCCGTATACCACATGGCTGTCGACCATTTCAGACACAGATGCACTGCTGGCTGAGTGGGACAAAAGCGGTGTTGTTACTGTTGATATGGGAGGTCACATCAGGCTTTGGGAAACTGGACTTGAACGTCTGCAGCGATCACTCATGGAATGGAGAAACATGATTGGACAAGATGACAGAAATATGCAGATAACAATCAACAGAGACAGTGGTGAAGATGTAAGCTCCCCCAAACACGGGAAGGAGGACCCAGACAACATGCCTCACGTGGGCGGCAACACTTGGGCTGGCGGAACAGGGGGAAGAGACACGGCAGGCCTGGGTGGCAAAGGAGGCCCTTACCGGCTGGATGCAGGCCATACGGTGTACCAGGTCTCTCAGGCTGAGAAAGATGCAGTTCCCGAAGAGGTCAAGAGAGCTGCTAGAGAGATGGGCCAGAGAGCATTCCAGCAGAGGCTAAAGGAGATCCAAATGAGTGAATACGATGCTGCAACCTATGAAAGGTTTTCAGGTGCTGTTCGGCGACAGGTGCACTCCCTCCGAATCATCCTGGATAATTTACAGGCTAAAGGTAAAGAAAGACAATGGCTAAGACATCAAGCTACTGGAGAATTAGATGATGCCAAGATCATTGATGGGCTGACTGGAGAAAAAGCCATCTACAAACGTCGGGGTGAGCTGGAGCCACAACTTGGCAGCCCACAACAGAAACCCAAGCGTCTGCGCCTGGTGGTAGATGTGTCTGGTAGCATGTACCGTTTCAACAGGATGGATGGCCGGCTTGAGCGCACAATGGAGGCTGTGTGTATGGTCATGGAAGCCTTCGAGAACTATGAGGAGAAGTTCCAGTATGACATCGTTGGACACTCTGGAGATGGCTACAACATTGGTCTGGTTCCAATGAACAAAATCCCCAAGGACAATAAGCAAAGACTAGAAATTCTGAAGACAATGCATGCCCACTCTCAGTTCTGCATGAGTGGGGACCACACGTTAGAAGGGACAGAACATGCCATCAAGGAAATTGTCAAAGAAGAAGCTGATGAGTACTTTGTCATAGTCTTGAGTGATGCAAATCTGTCACGATATGGAATACATCCTGCTAAGTTTGCTCAAATCCTCACAAGAGACCCTCAAGTAAATGCTTTTGCCATTTTTATTGGCTCTTTAGGTGATCAAGCAACCAGGCTTCAGAGAACTTTACCAGCTGGTCGGTCTTTCGTTGCCATGGATACCAAGGATATCCCTCAGATTTTACAACAGATCTTCACCTCCACCATGTTGTCGAGTGTCGCGGCCGC
Amino Acid Sequence
View Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSATMQSR
LLLLGAPGGHGGPASRRMRLLLRQVVQRRPGGDRQRPEVRLLHAGSGADT
GDTVNIGDVSYKLKIPKNPELVPQNYISDSLAQSVVQHLRWIMQKDLLGQ
DVFLIGPPGPLRRSIAMQYLELTKREVEYIALSRDTTETDLKQRREIRAG
TAFYIDQCAVRAATEGRTLILEGLEKAERNVLPVLNNLLENREMQLEDGR
FLMSAERYDKLLRDHTKKELDSWKIVRVSENFRVIALGLPVPRYSGNPLD
PPLRSRFQARDIYYLPFKDQLKLLYSIGANVSAEKVSQLLSFATTLCSQE
SSTLGLPDFPLDSLAAAVQILDSFPMMPIKHAIQWLYPYSILLGHEGKMA
VEGVLKRFELQDSGSSLLPKEIVKVEKMMENHVSQASVTIRIADKEVTIK
VPAGTRLLSQPCASDRFIQTLSHKQLQAEMMQSHMVKDICLIGGKGCGKT
VIAKNFADTLGYNIEPIMLYQDMTARDLLQQRYTLPNGDTAWRSSPLVNA
ALEGKLVLLDGIHRVNAGTLAVLQRLIHDRELSLYDGSRLLREDRYMRLK
EELQLSDEQLQKRSIFPIHPSFRIIALAEPPVIGSTAHQWLGPEFLTMFF
FHYMKPLVKSEEIQVIKEKVPNVPQEALDKLLSFTHKLRETQDPTAQSLA
ASLSTRQLLRISRRLSQYPNENLHSAVTKACLSRFLPSLARSALEKNLAD
ATIEINTDDNLEPELKDYKCEVTSGTLRIGAVSAPIYNAHEKMKVPDVLF
YDNIQHVIVMEDMLKDFLLGEHLLLVGNQGVGKNKIVDRFLHLLNRPREY
IQLHRDTTVQTLTLQPSVKDGLIVYEDSPLVKAVKLGHILVVDEADKAPT
NVTCILKTLVENGEMILADGRRIVANSANVNGRENVVVIHPDFRMIVLAN
RPGFPFLGNDFFGTLGDIFSCHAVDNPKPHSELEMLRQYGPNVPEPILQK
LVAAFGELRSLADQGIINYPYSTREVVNIVKHLQKFPTEGLSSVVRNVFD
FDSYNNDMREILINTLHKYGIPIGAKPTSVQLAKELTLPEQTFMGYWTIG
QARSGMQKLLCPVETHHIDIKGPALINIQEYPIERHEERSLNFTEECASW
RIPLDEINIICDIATSHENEQNTLYVVTCNPASLYFMNMTGKSGFFVDFF
DIFPRTANGVWHPFVTVAPLGSPLKGQVVLHEQQSNVILLLDTTGRALHR
LILPSEKFTSKKPFWWNKEEAETYKMCKEFSHKNWLVFYKEKGNSLTVLD
VLEGRTHTISLPINLKTVFLVAEDKWLLVESKTNQKYLLTKPAHIESEGS
GVCQLYVLKEEPPSTGFGVTQETEFSIPHKISSDQLSSEHLSSAVEQKIA
SPNRILSDEKNYATIVVGFPDLMSPSEVYSWKRPSSLHKRSGTDTSFYRG
KKKRGTPKQSNCVTLLDTNQVVRILPPGEVPLKDIYPKDVTPPQTSGYIE
VTDLQSKKLRYIPIPRSESLSPYTTWLSTISDTDALLAEWDKSGVVTVDM
GGHIRLWETGLERLQRSLMEWRNMIGQDDRNMQITINRDSGEDVSSPKHG
KEDPDNMPHVGGNTWAGGTGGRDTAGLGGKGGPYRLDAGHTVYQVSQAEK
DAVPEEVKRAAREMGQRAFQQRLKEIQMSEYDAATYERFSGAVRRQVHSL
RIILDNLQAKGKERQWLRHQATGELDDAKIIDGLTGEKAIYKRRGELEPQ
LGSPQQKPKRLRLVVDVSGSMYRFNRMDGRLERTMEAVCMVMEAFENYEE
KFQYDIVGHSGDGYNIGLVPMNKIPKDNKQRLEILKTMHAHSQFCMSGDH
TLEGTEHAIKEIVKEEADEYFVIVLSDANLSRYGIHPAKFAQILTRDPQV
NAFAIFIGSLGDQATRLQRTLPAGRSFVAMDTKDIPQILQQIFTSTMLSS
VAAAYPYDVPDYA*
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSATMQSR
LLLLGAPGGHGGPASRRMRLLLRQVVQRRPGGDRQRPEVRLLHAGSGADT
GDTVNIGDVSYKLKIPKNPELVPQNYISDSLAQSVVQHLRWIMQKDLLGQ
DVFLIGPPGPLRRSIAMQYLELTKREVEYIALSRDTTETDLKQRREIRAG
TAFYIDQCAVRAATEGRTLILEGLEKAERNVLPVLNNLLENREMQLEDGR
FLMSAERYDKLLRDHTKKELDSWKIVRVSENFRVIALGLPVPRYSGNPLD
PPLRSRFQARDIYYLPFKDQLKLLYSIGANVSAEKVSQLLSFATTLCSQE
SSTLGLPDFPLDSLAAAVQILDSFPMMPIKHAIQWLYPYSILLGHEGKMA
VEGVLKRFELQDSGSSLLPKEIVKVEKMMENHVSQASVTIRIADKEVTIK
VPAGTRLLSQPCASDRFIQTLSHKQLQAEMMQSHMVKDICLIGGKGCGKT
VIAKNFADTLGYNIEPIMLYQDMTARDLLQQRYTLPNGDTAWRSSPLVNA
ALEGKLVLLDGIHRVNAGTLAVLQRLIHDRELSLYDGSRLLREDRYMRLK
EELQLSDEQLQKRSIFPIHPSFRIIALAEPPVIGSTAHQWLGPEFLTMFF
FHYMKPLVKSEEIQVIKEKVPNVPQEALDKLLSFTHKLRETQDPTAQSLA
ASLSTRQLLRISRRLSQYPNENLHSAVTKACLSRFLPSLARSALEKNLAD
ATIEINTDDNLEPELKDYKCEVTSGTLRIGAVSAPIYNAHEKMKVPDVLF
YDNIQHVIVMEDMLKDFLLGEHLLLVGNQGVGKNKIVDRFLHLLNRPREY
IQLHRDTTVQTLTLQPSVKDGLIVYEDSPLVKAVKLGHILVVDEADKAPT
NVTCILKTLVENGEMILADGRRIVANSANVNGRENVVVIHPDFRMIVLAN
RPGFPFLGNDFFGTLGDIFSCHAVDNPKPHSELEMLRQYGPNVPEPILQK
LVAAFGELRSLADQGIINYPYSTREVVNIVKHLQKFPTEGLSSVVRNVFD
FDSYNNDMREILINTLHKYGIPIGAKPTSVQLAKELTLPEQTFMGYWTIG
QARSGMQKLLCPVETHHIDIKGPALINIQEYPIERHEERSLNFTEECASW
RIPLDEINIICDIATSHENEQNTLYVVTCNPASLYFMNMTGKSGFFVDFF
DIFPRTANGVWHPFVTVAPLGSPLKGQVVLHEQQSNVILLLDTTGRALHR
LILPSEKFTSKKPFWWNKEEAETYKMCKEFSHKNWLVFYKEKGNSLTVLD
VLEGRTHTISLPINLKTVFLVAEDKWLLVESKTNQKYLLTKPAHIESEGS
GVCQLYVLKEEPPSTGFGVTQETEFSIPHKISSDQLSSEHLSSAVEQKIA
SPNRILSDEKNYATIVVGFPDLMSPSEVYSWKRPSSLHKRSGTDTSFYRG
KKKRGTPKQSNCVTLLDTNQVVRILPPGEVPLKDIYPKDVTPPQTSGYIE
VTDLQSKKLRYIPIPRSESLSPYTTWLSTISDTDALLAEWDKSGVVTVDM
GGHIRLWETGLERLQRSLMEWRNMIGQDDRNMQITINRDSGEDVSSPKHG
KEDPDNMPHVGGNTWAGGTGGRDTAGLGGKGGPYRLDAGHTVYQVSQAEK
DAVPEEVKRAAREMGQRAFQQRLKEIQMSEYDAATYERFSGAVRRQVHSL
RIILDNLQAKGKERQWLRHQATGELDDAKIIDGLTGEKAIYKRRGELEPQ
LGSPQQKPKRLRLVVDVSGSMYRFNRMDGRLERTMEAVCMVMEAFENYEE
KFQYDIVGHSGDGYNIGLVPMNKIPKDNKQRLEILKTMHAHSQFCMSGDH
TLEGTEHAIKEIVKEEADEYFVIVLSDANLSRYGIHPAKFAQILTRDPQV
NAFAIFIGSLGDQATRLQRTLPAGRSFVAMDTKDIPQILQQIFTSTMLSS
VAAAYPYDVPDYA*
Vector Type
Mammalian
Antibiotic
Amp
DU Number
DU49914
Unit Source
Location
PPU
MTA
Notes
Made as intermediate. N.B. kozak context on VWA8 initiating methionine.
N.B. Recommend propagation at 30 degrees.
N.B. Recommend propagation at 30 degrees.
Price
£125.00
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