cDNA Clone TRABID

• Plasmid: pcDNA FRT TO 3xFLAG TRABID V171A C443A
• Parent Plasmid: pcDNA5 FRT/TO 3FLAG1
• Expressed: 3XFLAG TRABID V171A C443A
• Genbank: NM_017580.2
• Tag: 3FLAG

Sequence of Insert

gcggccgcATGTCAGAACGTGGAATTAAGTGGGCTTGTGAATATTGTACGTATGAAAACTGGCCATCTGCAATCAAGTGTACTATGTGTCGTGCCCAAAGACCTAGTGGAACAATTATTACAGAAGATCCATTTAAAAGTGGTTCAAGTGATGTTGGTAGAGATTGGGATCCTTCCAGCACCGAAGGAGGAAGTAGTCCTTTGATATGTCCAGACTCTAGTGCAAGACCAAGGGTGAAATCTTCGTATAGCATGGAAAATGCAAATAAGTGGTCATGCCACATGTGTACATATTTGAACTGGCCAAGAGCAATCAGATGTACCCAGTGCTTATCCCAACGTAGGACCAGGAGTCCTACAGAATCTCCTCAGTCCTCAGGATCTGGCTCAAGACCAGTTGCTTTTTCTGTTGATCCTTGTGAGGAATACAATGATAGAAATAAACTGAACACTAGGACACAGCACTGGACTTGCTCTGTTTGCACATATGAAAACTGGGCCAAGGCTAAAAGATGTGTTGCTTGTGATCATCCCAGACCTAATAACATTGAAGCAATAGAATTGGCAGAGACTGAAGAGGCTTCTTCAATAATAAATGAGCAAGACAGAGCTCGATGGAGGGGAAGTTGCAGTAGTGGTAATAGCCAAAGGAGATCACCTCCTGCTACGAAGCGGGACTCTGAAGTGAAAATGGATTTTCAGAGGATTGAATTGGCTGGTGCTGTGGGAAGCAAGGAGGAACTTGAAGTAGACTTTAAAAAACTAAAGCAAATTAAAAACAGGATGAAAAAGACTGATTGGCTCTTCCTCAATGCTTGTGTGGGGGTTGTAGAAGGTGATTTAGCTGCCATAGAAGCATACAAGTCATCAGGAGGAGACATTGCACGTCAGCTCACCGCAGATGAAGTACGCTTGCTGAATCGTCCTTCTGCCTTTGATGTTGGCTATACTCTTGTACACTTGGCTATACGTTTTCAGAGGCAGGATATGCTAGCAATATTGCTTACAGAGGTGTCTCAACAAGCAGCAAAGTGTATTCCAGCAATGGTGTGTCCTGAACTGACAGAACAAATCCGGAGAGAGATAGCTGCCTCTCTTCATCAGAGAAAGGGGGATTTTGCTTGCTATTTTCTGACTGACCTTGTAACATTTACATTGCCAGCAGATATTGAAGATTTGCCCCCAACAGTCCAAGAAAAATTATTTGATGAGGTGCTTGATAGAGACGTTCAAAAAGAATTAGAAGAAGAATCTCCAATTATTAACTGGTCCTTGGAATTGGCTACACGTTTGGACAGTCGACTGTATGCACTTTGGAACCGGACTGCAGGAGACGCCCTACTTGATTCAGTTCTACAAGCTACCTGGGGCATCTATGACAAGGACTCAGTGCTTCGGAAAGCCCTGCATGACAGCCTGCATGACTGTTCACATTGGTTTTACACACGCTGGAAAGATTGGGAATCATGGTATTCTCAGAGCTTTGGTTTACATTTTTCCTTGAGAGAAGAACAGTGGCAAGAAGACTGGGCATTTATACTCTCTCTTGCTAGTCAGCCTGGAGCAAGCTTGGAGCAGACGCACATTTTTGTACTGGCACATATTCTTAGACGACCAATTATAGTTTATGGAGTAAAATATTACAAGAGTTTCCGGGGAGAAACTTTAGGATATACTCGGTTTCAAGGTGTTTATCTGCCTTTGTTGTGGGAACAGAGTTTTTGTTGGAAAAGTCCGATTGCTCTGGGTTATACGAGGGGCCACTTCTCTGCTTTGGTTGCCATGGAAAATGATGGCTATGGCAACCGAGGTGCTGGTGCTAATCTCAATACCGATGATGATGTCACCATCACATTTTTGCCTCTGGTTGACAGTGAAAGGAAGCTACTCCATGTGCACTTCCTTTCTGCTCAGGAGCTAGGTAATGAGGAACAGCAAGAAAAACTGCTCAGGGAGTGGCTGGACTGCTGTGTGACGGAGGGGGGAGTTCTGGTTGCCATGCAGAAGAGTTCTCGGCGGCGAAATCACCCCCTGGTCACTCAGATGGTAGAAAAATGGCTTGACCGCTACCGACAGATCCGGCCGTGTACATCCCTGTCTGATGGAGAGGAAGATGAGGATGATGAAGATGAATAAgcggccgc

Amino Acid Sequence

MDYKDDDDKGSDYKDDDDKDYKDDDDKGSPEFPGRLEAAAMSERGIKWACEYCTYENWPSAIKCTMCRAQRPSGTIITEDPFKSGSSDVGRDWDPSSTEGGSSPLICPDSSARPRVKSSYSMENANKWSCHMCTYLNWPRAIRCTQCLSQRRTRSPTESPQSSGSGSRPVAFSVDPCEEYNDRNKLNTRTQHWTCSVCTYENWAKAKRCVACDHPRPNNIEAIELAETEEASSIINEQDRARWRGSCSSGNSQRRSPPATKRDSEVKMDFQRIELAGAVGSKEELEVDFKKLKQIKNRMKKTDWLFLNACVGVVEGDLAAIEAYKSSGGDIARQLTADEVRLLNRPSAFDVGYTLVHLAIRFQRQDMLAILLTEVSQQAAKCIPAMVCPELTEQIRREIAASLHQRKGDFACYFLTDLVTFTLPADIEDLPPTVQEKLFDEVLDRDVQKELEEESPIINWSLELATRLDSRLYALWNRTAGDALLDSVLQATWGIYDKDSVLRKALHDSLHDCSHWFYTRWKDWESWYSQSFGLHFSLREEQWQEDWAFILSLASQPGASLEQTHIFVLAHILRRPIIVYGVKYYKSFRGETLGYTRFQGVYLPLLWEQSFCWKSPIALGYTRGHFSALVAMENDGYGNRGAGANLNTDDDVTITFLPLVDSERKLLHVHFLSAQELGNEEQQEKLLREWLDCCVTEGGVLVAMQKSSRRRNHPLVTQMVEKWLDRYRQIRPCTSLSDGEEDEDDEDE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU47129
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please use DU49089 as the template for the site directed mutagenesis. Thank you.