cDNA Clone Tof2

• Plasmid: pGADT7 TOF2 Q505A E506A I508A L511A
• Parent Plasmid: pGADT7
• Expressed: GAL4 AD TOF2 Q505A E506A I508A L511A
• Genbank: NP_012935.3
• Tag: GAL4 DNA-AD
• Species: Yeast

Sequence of Insert

GAATTCGCCCTTATGATAAAAATGTGGAGGTTACAGATTGTACTGGTGCCGCCAAGTGCTCAAGATATTATCACATTTCTCGAAGCACGCTTGAATACTCCACAATCAGTGTCTCCTATGGTCCAGTATAATGAAGATATAATTACGCACAACAACAGTATCAATAACTGTAGCGACCCCTCTCCTACGAGCCCGTCTTCGCAAAATTCTATTCAGTCGAATCGTAGTAGTGATTTCATTAACTACCTCCCGAATTGTAAAAAGTTTTTACATTTTACGGACGGTGACAATACCCTTTTACAACTATCTAATGAAATTTTAACTAAATTTGATAGACTTTATCCAAATTTCAAAGAATCCATTGAAATCGTATCTCTACAAGATAGGCATGGTTGTGATTTGGACTCTGAATTTATAATAAAAGACGTATTTGAAAATGATGGTGTGGTTTTGGTAATTTTAAAAGATGAATTAGACTGGAGTAGAAATCAACATATTTCACTTTTACAATTGGCAAGACAGCGGAGACGGCAGGACAACAAGCCATCCACAAAATCCATCGTTACTGAAAAGAGAAAAAAGATCTCTAAGGAAGACTTATCTTCTATATCAAATAAAGATACAATGCATCTAATAGCTAAAAGCTCGCTCAAGAATAACTTCATTAATAAATCAAGAGTATCAACGCCGCTAATGAACGAAATTCTACCGCTTGCAAGCAAGTATGATGCACTGAATAAAGAAAAGTGTCCCATGCCACTTACTTCCACAGTAGTGGCATCAAATGTACACAAAGACGTAAAAGATCACGCTCGTGCTAAAGAGGGAGTTGTAACGCAAGGGAGTGATAACAATAAAGAAAATATACCTTCATCCACTCAACAACAGAAAAATGATGGCGCTAAAAGGGCCGAATCAAAGGATTTAGATCTTTTACGCAATTCGTCAGAAGATGCTGATTATGAACCCGCAGATGAAAACTCCCCTCAAATTTCGTTTGATTCGATTGATACCGACTTCCAATTGAGCACTACTTCTCATACTAATTCTGATATGCATATACAATATTCTAACCCCTCTAGTGGTGCTCATAGTCCCAGGAAGAGTTCCTTAGAAATTAAAGTTCAAAACAAAAAAGGTGATGATCTGCCATTGAACGACAAAGATATAGGTGAGAATTGTAGAAGGATAGAAGCATTTTCAGATGAAGAGGATTTTAATGAAACCGATAATGATCGTGCGGATTCGTTTATCAACAATTCTAAGAAGGCATCGATGGGATTTCGGGATATCAATTCCGACTTGGATAGCGTTTCATTCAACAGTGATATTGAAAATGCAGTACAAAGTACTCAAAGTACCAAAAATGTTGTTTCTCCTCCCTTTTTTCCTGAAAAAGAATTGAATAATCGGTTACATCAAAGTCAAGGTAAGGAAGCCCTATTTCGACTTGTTGAGAAGGAGTTTCCTGATAAATCATTGGGTGCTGCGTCATCTACTTCGCATGCAAAAGACGTAAAAATAGCCGCTACTGCCCGGAAAGCTAATAGGTTCAAGCCAACTGGGGAAACTAAGGTCCAAAAAAGGAATAGTATTACGGAACCATATTACGGAAAATTTGGAATAATGAAGAAAGATAAACCCAAATCCATAACTTCAAAAGGGGTAAGTCTGGAAACAAAGCACTTTGATGATCCCAATACCATTATTTCCGGTGAGAAATTCGCAAAATTTGGTAAAATAAAAGTGAAACGAAAAACTGATGATGTTGGATCGAAAGTTATAGAGTTTAAAAGAAAAAGAAATATGGGGAATAGATCCCTAAAAGATATTTTTGCCAATGCTGGGAAACCGCCAAATGCAGCTTCGACCATAAAAGTAGTCAAATTAATGAGAGATCCTGTTGATAATAGTAAAGACAAAGTGGAGGCAACTTCTAATAGTACGGCTCAGGAACAAGAACAAGTCTCACCCAAGTTGCCAGTTATGAATAGTACTCCTGGAAAAAGAAAAAATGGCCAAGCGATACCGTCCAGTTTAGAAAGAACACCGCAGCTCAAGAAAGTCAAGGTTACCAGGTCGCATTCTTCACCTTCTTCTTCTTCTTCCATGTCACTTGAATCTTCATTGGATAGCTCTTCCTCAGATGATTCGGATGATGATTCTGATTCGAGGAACGTGCAAGTTAAAAAAATTAATTTCAAAACAAGTCATGGACCAGCTGGAAATTCTAATGGAAAACCAATGCTGGATGTGGATGACAATGAAATAAATACAAAGAAGTACCAAACACCTAAATATGTTGAAAGTGATGAAGACGACCAGTAAAAGGGCGAATTC

Amino Acid Sequence

MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIFNTTHYRASAAMEYPYDVPDYAHMAMEASEFALMIKMWRLQIVLVPPSAQDIITFLEARLNTPQSVSPMVQYNEDIITHNNSINNCSDPSPTSPSSQNSIQSNRSSDFINYLPNCKKFLHFTDGDNTLLQLSNEILTKFDRLYPNFKESIEIVSLQDRHGCDLDSEFIIKDVFENDGVVLVILKDELDWSRNQHISLLQLARQRRRQDNKPSTKSIVTEKRKKISKEDLSSISNKDTMHLIAKSSLKNNFINKSRVSTPLMNEILPLASKYDALNKEKCPMPLTSTVVASNVHKDVKDHARAKEGVVTQGSDNNKENIPSSTQQQKNDGAKRAESKDLDLLRNSSEDADYEPADENSPQISFDSIDTDFQLSTTSHTNSDMHIQYSNPSSGAHSPRKSSLEIKVQNKKGDDLPLNDKDIGENCRRIEAFSDEEDFNETDNDRADSFINNSKKASMGFRDINSDLDSVSFNSDIENAVQSTQSTKNVVSPPFFPEKELNNRLHQSQGKEALFRLVEKEFPDKSLGAASSTSHAKDVKIAATARKANRFKPTGETKVQKRNSITEPYYGKFGIMKKDKPKSITSKGVSLETKHFDDPNTIISGEKFAKFGKIKVKRKTDDVGSKVIEFKRKRNMGNRSLKDIFANAGKPPNAASTIKVVKLMRDPVDNSKDKVEATSNSTAQEQEQVSPKLPVMNSTPGKRKNGQAIPSSLERTPQLKKVKVTRSHSSPSSSSSMSLESSLDSSSSDDSDDDSDSRNVQVKKINFKTSHGPAGNSNGKPMLDVDDNEINTKKYQTPKYVESDEDDQ*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU26112
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent mutation at S661 to KO internal EcoR1 site.