Plasmid
pET28a 8HIS SKP1 Dia2 G199-end (yeast)
Parent Plasmid
pET28a original
Expressed
yeast Dia2 G199-S732(end)
Genbank
NP_014723.2
Tag
6His
Species
Yeast
Sequence of Insert
View Sequence
ATGGGTAGTACCAAGAAAACTGATTTAGTTGGCAATTTGCCGATAGAAATACTACCTATCATATTCCAAAGGTTTACCACTAAGGAGCTTGTTACGTTGTCGCTAGTTTGTAACAAATGGAGGGACAAAATTTTGTATCATTTAGACTGCTTCCAAGAATTCAACTTGGCACCAATAAATTTTAAAAATTTTGTAAAGTTTATGGATTTTTTACAACAGAATTTCACCAGGACGTATAGAAAATATATTCTTTCACAAGTAAAAGTCAGTTCTAGAATCACTTCGGAAGAGTTACGAATAACTCAATTATTATTCAGTAAAATGCCGAAATGTATAAACATCGAACGGCTTATACTGTCAATGCCAACACTAACTACAACACAAATCTTTAAGTTAATGGTTAGAGGAGGCACAGATTTTTTTACAAGGCTGCTGGAATTATCTCTCATGATAACTTATAGGCCTGATAAACAACATGAATTGGAGATACTGCAGACTTGTCCTCTGTTGAAAAAAATAGAATTAATTTTTGTCAATTCACTGGTACCAATTTTCGACGGAAATAATAGCGTTGGGAGGGATGGAAGTTTCAATGTGATGGCCCGTCATACTAATATGCAGATATCCACTGCGGATAATGATGAACAGGGAATAGTAGAAGAAAAGGTGATTTATAGTGAGCTAGAGAAGATAACATTGATATGTGATAAGAAGAAAATTAAGAATTTTCCACTTTGTCGTGCTCTATTAAGAGGCCAGTTTCCTCTATTGCAGAAACTGACGATAACTGGTGTAACATTTCCTATGAATAATCAAGACATAATGAATTTTCAATGGCTATTGAACTTCCCAGACCTAAAAGAACTATGGATAGAAGATAACGACAACTGTGAACTCAGTAAGTTTTTGCAATTGTTAAAATTTTCGAATGTCTGGAAAAACTTGGAAAAGTTAACCTTTAGAGAGAACAAGCTATATCCAATTGTTAATTTGGATGAAGATCAGCCTGTTACGAATGACGACGAGGTGCCATCCATGCTATTTTACAAGGAAAATTTCCAAAACCTGGAAAAGTTGGATCTAATGGGGACATCGATAAGTGGTTCTGCGTTGACTAGATTATGCGAGCAGGAATACTTGGATGGTCGGAAATTAAGAAGTTTAAACATTGGGAATTGCCCCAACATCCAGTTCCCAAATAATCACGCTCATACAGCAAGGATGATACTGGATGTGAATGCAGTTTTGAAGAGACTCTCCAAGTTGGAAGAAATAAATTTATCGCATCTCAGCTCCTTGAATGATAGTACGATGAAGTCATTCATTATTAATGTACCATTTTTGGAAAATTTAAAGAGGCTTGACATTTCGCACAATTTTGAAATTACAGGAATATCAATATATGAATTTCTAAAAAAATTTCAGATGGATCATGACAACGAAGCGGGAGGTCAACCACTGGCATATTTAAATATTGACGGATGTTCCCAAGTGTCTCATATCACAGTTAATATGATCCGAGCGCAGAACTTAGTTACTCAAGTGGATTGTGTTTATGAAAGAGATGTATGGAGAAAATTTGGAATTAATTCATATTCATACTCATAGGCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHHHENLYFQGGSMVTSNVVLVSGEGERFTVDKKIAERSLLLKNYLNDMHDSNLQNNSDSESDSDSETNHKSKDNNNGDDDDEDDDEIVMPVPNVRSSVLQKVIEWAEHHRDSNFPDEDDDDSRKSAPVDSWDREFLKVDQEMLYEIILAANYLNIKPLLDAGCKVVAEMIRGRSPEEIRRTFNIVNDFTPEEEAAIRRENEWAEDR*AAAMGSTKKTDLVGNLPIEILPIIFQRFTTKELVTLSLVCNKWRDKILYHLDCFQEFNLAPINFKNFVKFMDFLQQNFTRTYRKYILSQVKVSSRITSEELRITQLLFSKMPKCINIERLILSMPTLTTTQIFKLMVRGGTDFFTRLLELSLMITYRPDKQHELEILQTCPLLKKIELIFVNSLVPIFDGNNSVGRDGSFNVMARHTNMQISTADNDEQGIVEEKVIYSELEKITLICDKKKIKNFPLCRALLRGQFPLLQKLTITGVTFPMNNQDIMNFQWLLNFPDLKELWIEDNDNCELSKFLQLLKFSNVWKNLEKLTFRENKLYPIVNLDEDQPVTNDDEVPSMLFYKENFQNLEKLDLMGTSISGSALTRLCEQEYLDGRKLRSLNIGNCPNIQFPNNHAHTARMILDVNAVLKRLSKLEEINLSHLSSLNDSTMKSFIINVPFLENLKRLDISHNFEITGISIYEFLKKFQMDHDNEAGGQPLAYLNIDGCSQVSHITVNMIRAQNLVTQVDCVYERDVWRKFGINSYSYS*
Vector Type
Bacterial
Antibiotic
Amp
DU Number
DU25908
Unit Source
Location
PPU
MTA
Notes
Has non silent mutation to correct.
We would like to clone a fragment of Dia2 G199-S732(end) into a pET28a vector already containing full length Skp1 (available as DU49865) to have a dual expressing vector for E.coli expression.
We would like to clone a fragment of Dia2 G199-S732(end) into a pET28a vector already containing full length Skp1 (available as DU49865) to have a dual expressing vector for E.coli expression.
Price
£125.00
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