cDNA Clone TRABID

• Plasmid: pcDNA FRT TO GFP TRABID M26A Q106A V171A
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP TRABID M26A Q106A V171A
• Genbank: NM_017580.2
• Tag: GFP

Sequence of Insert

ATGTCAGAACGTGGAATTAAGTGGGCTTGTGAATATTGTACGTATGAAAACTGGCCATCTGCAATCAAGTGTACTGCGTGTCGTGCCCAAAGACCTAGTGGAACAATTATTACAGAAGATCCATTTAAAAGTGGTTCAAGTGATGTTGGTAGAGATTGGGATCCTTCCAGCACCGAAGGAGGAAGTAGTCCTTTGATATGTCCAGACTCTAGTGCAAGACCAAGGGTGAAATCTTCGTATAGCATGGAAAATGCAAATAAGTGGTCATGCCACATGTGTACATATTTGAACTGGCCAAGAGCAATCAGATGTACCGCGTGCTTATCCCAACGTAGGACCAGGAGTCCTACAGAATCTCCTCAGTCCTCAGGATCTGGCTCAAGACCAGTTGCTTTTTCTGTTGATCCTTGTGAGGAATACAATGATAGAAATAAACTGAACACTAGGACACAGCACTGGACTTGCTCTGTTTGCACATATGAAAACTGGGCCAAGGCTAAAAGATGTGTTGCTTGTGATCATCCCAGACCTAATAACATTGAAGCAATAGAATTGGCAGAGACTGAAGAGGCTTCTTCAATAATAAATGAGCAAGACAGAGCTCGATGGAGGGGAAGTTGCAGTAGTGGTAATAGCCAAAGGAGATCACCTCCTGCTACGAAGCGGGACTCTGAAGTGAAAATGGATTTTCAGAGGATTGAATTGGCTGGTGCTGTGGGAAGCAAGGAGGAACTTGAAGTAGACTTTAAAAAACTAAAGCAAATTAAAAACAGGATGAAAAAGACTGATTGGCTCTTCCTCAATGCTTGTGTGGGGGTTGTAGAAGGTGATTTAGCTGCCATAGAAGCATACAAGTCATCAGGAGGAGACATTGCACGTCAGCTCACCGCAGATGAAGTACGCTTGCTGAATCGTCCTTCTGCCTTTGATGTTGGCTATACTCTTGTACACTTGGCTATACGTTTTCAGAGGCAGGATATGCTAGCAATATTGCTTACAGAGGTGTCTCAACAAGCAGCAAAGTGTATTCCAGCAATGGTGTGTCCTGAACTGACAGAACAAATCCGGAGAGAGATAGCTGCCTCTCTTCATCAGAGAAAGGGGGATTTTGCTTGCTATTTTCTGACTGACCTTGTAACATTTACATTGCCAGCAGATATTGAAGATTTGCCCCCAACAGTCCAAGAAAAATTATTTGATGAGGTGCTTGATAGAGACGTTCAAAAAGAATTAGAAGAAGAATCTCCAATTATTAACTGGTCCTTGGAATTGGCTACACGTTTGGACAGTCGACTGTATGCACTTTGGAACCGGACTGCAGGAGACTGCCTACTTGATTCAGTTCTACAAGCTACCTGGGGCATCTATGACAAGGACTCAGTGCTTCGGAAAGCCCTGCATGACAGCCTGCATGACTGTTCACATTGGTTTTACACACGCTGGAAAGATTGGGAATCATGGTATTCTCAGAGCTTTGGTTTACATTTTTCCTTGAGAGAAGAACAGTGGCAAGAAGACTGGGCATTTATACTCTCTCTTGCTAGTCAGCCTGGAGCAAGCTTGGAGCAGACGCACATTTTTGTACTGGCACATATTCTTAGACGACCAATTATAGTTTATGGAGTAAAATATTACAAGAGTTTCCGGGGAGAAACTTTAGGATATACTCGGTTTCAAGGTGTTTATCTGCCTTTGTTGTGGGAACAGAGTTTTTGTTGGAAAAGTCCGATTGCTCTGGGTTATACGAGGGGCCACTTCTCTGCTTTGGTTGCCATGGAAAATGATGGCTATGGCAACCGAGGTGCTGGTGCTAATCTCAATACCGATGATGATGTCACCATCACATTTTTGCCTCTGGTTGACAGTGAAAGGAAGCTACTCCATGTGCACTTCCTTTCTGCTCAGGAGCTAGGTAATGAGGAACAGCAAGAAAAACTGCTCAGGGAGTGGCTGGACTGCTGTGTGACGGAGGGGGGAGTTCTGGTTGCCATGCAGAAGAGTTCTCGGCGGCGAAATCACCCCCTGGTCACTCAGATGGTAGAAAAATGGCTTGACCGCTACCGACAGATCCGGCCGTGTACATCCCTGTCTGATGGAGAGGAAGATGAGGATGATGAAGATGAATAA

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSPGEFASLEKLAAAMSERGIKWACEYCTYENWPSAIKCTACRAQRPSGTIITEDPFKSGSSDVGRDWDPSSTEGGSSPLICPDSSARPRVKSSYSMENANKWSCHMCTYLNWPRAIRCTACLSQRRTRSPTESPQSSGSGSRPVAFSVDPCEEYNDRNKLNTRTQHWTCSVCTYENWAKAKRCVACDHPRPNNIEAIELAETEEASSIINEQDRARWRGSCSSGNSQRRSPPATKRDSEVKMDFQRIELAGAVGSKEELEVDFKKLKQIKNRMKKTDWLFLNACVGVVEGDLAAIEAYKSSGGDIARQLTADEVRLLNRPSAFDVGYTLVHLAIRFQRQDMLAILLTEVSQQAAKCIPAMVCPELTEQIRREIAASLHQRKGDFACYFLTDLVTFTLPADIEDLPPTVQEKLFDEVLDRDVQKELEEESPIINWSLELATRLDSRLYALWNRTAGDCLLDSVLQATWGIYDKDSVLRKALHDSLHDCSHWFYTRWKDWESWYSQSFGLHFSLREEQWQEDWAFILSLASQPGASLEQTHIFVLAHILRRPIIVYGVKYYKSFRGETLGYTRFQGVYLPLLWEQSFCWKSPIALGYTRGHFSALVAMENDGYGNRGAGANLNTDDDVTITFLPLVDSERKLLHVHFLSAQELGNEEQQEKLLREWLDCCVTEGGVLVAMQKSSRRRNHPLVTQMVEKWLDRYRQIRPCTSLSDGEEDEDDEDE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU47182
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please use DU45864 as the template for the site directed mutagenesis. Thank you.