cDNA Clone SAPK4

• Synonyms: MAPK13
• Plasmid: pGEX4T SAPK4 D168A
• Parent Plasmid: pGEX4T1
• Expressed: GST-SAPK4 D168A
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGAGCCTCATCCGGAAAAAGGGCTTCTACAAGCAGGACGTCAACAAGACAGCCTGGGAGCTGCCCAAGACCTACGTGTCCCCGACGCACGTCG
GCAGCGGGGCCTATGGCTCCGTGTGCTCGGCCATCGACAAGCGGTCAGGGGAGAAGGTGGCCATCAAGAAGCTGAGCCGACCCTTTCAGTCCGAGATCTT
CGCCAAGCGCGCCTACCGGGAGCTGCTGCTGCTGAAGCACATGCAGCATGAGAACGTCATTGGGCTCCTGGATGTCTTCACCCCAGCCTCCTCCCTGCGC
AACTTCTATGACTTCTACCTGGTGATGCCCTTCATGCAGACGGATCTGCAGAAGATCATGGGGATGGAGTTCAGTGAGGAGAAGATCCAGTACCTGGTGT
ATCAGATGCTCAAAGGCCTTAAGTACATCCACTCTGCTGGGGTCGTGCACAGGGACCTGAAGCCAGGCAACCTGGCTGTGAATGAGGACTGTGAACTGAA
GATTCTGGCTTTTGGGCTGGCGCGACATGCAGACGCCGAGATGACTGGCTACGTGGTGACCCGCTGGTACCGAGCCCCCGAGGTGATCCTCAGCTGGATG
CACTACAACCAGACAGTGGACATCTGGTCTGTGGGCTGTATCATGGCAGAGATGCTGACAGGGAAAACTCTGTTCAAGGGGAAAGATTACCTGGACCAGC
TGACCCAGATCCTGAAAGTGACCGGGGTGCCTGGCACGGAGTTTGTGCAGAAGCTGAACGACAAAGCGGCCAAATCCTACATCCAGTCCCTGCCACAGAC
CCCCAGGAAGGATTTCACTCAGCTGTTCCCACGGGCCAGCCCCCAGGCTGCGGACCTGCTGGAGAAGATGCTGGAGCTAGACGTGGACAAGCGCCTGACG
GCCGCGCAGGCCCTCACCCATCCCTTCTTTGAACCCTTCCGGGACCCTGAGGAAGAGACGGAGGCCCAGCAGCCGTTTGATGATTCCTTAGAACACGAGA
AACTCACAGTGGATGAATGGAAGCAGCACATCTACAAGGAGATTGTGAACTTCAGCCCCATTGCCCGGAAGGACTCACGGCGCCGGAGTGGCATGAAGCT
GTAGGGACTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSPEFMSLIRKKGFYKQDVNKTAWEL
PKTYVSPTHVGSGAYGSVCSAIDKRSGEKVAIKKLSRPFQSEIFAKRAYR
ELLLLKHMQHENVIGLLDVFTPASSLRNFYDFYLVMPFMQTDLQKIMGME
FSEEKIQYLVYQMLKGLKYIHSAGVVHRDLKPGNLAVNEDCELKILAFGL
ARHADAEMTGYVVTRWYRAPEVILSWMHYNQTVDIWSVGCIMAEMLTGKT
LFKGKDYLDQLTQILKVTGVPGTEFVQKLNDKAAKSYIQSLPQTPRKDFT
QLFPRASPQAADLLEKMLELDVDKRLTAAQALTHPFFEPFRDPEEETEAQ
QPFDDSLEHEKLTVDEWKQHIYKEIVNFSPIARKDSRRRSGMKL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46809
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Already have mammalian kinase dead to subclone from