cDNA Clone SPAK

• Synonyms: STK39, DCHT, PASK
• Plasmid: pEBG HA SPAK Opt L493A
• Parent Plasmid: pEBG6P
• Expressed: GST-3C-HA-SPAK Opt L493A
• Genbank: AAC72238.1
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGTACCCATACGATGTGCCAGATTACGCCGCCGAGCCTAGCGGATCTCCCGTGCATGTGCAGCTGCCTCAGCAGGCCGCTCCTGTGACAGCTGCCGCTGCTGCTGCTCCAGCTGCTGCAACAGCAGCTCCTGCTCCTGCAGCACCAGCTGCCCCAGCTCCAGCCCCTGCCCCTGCACCAGCAGCACAGGCTGTGGGATGGCCCATCTGCAGAGATGCCTACGAGCTGCAGGAAGTGATCGGCAGCGGAGCCACAGCAGTGGTGCAGGCTGCCCTGTGCAAGCCCAGACAGGAACGGGTGGCCATCAAGCGGATCAACCTGGAAAAGTGCCAGACCAGCATGGACGAGCTGCTGAAAGAAATCCAGGCCATGAGCCAGTGCAGCCACCCCAACGTCGTGACCTACTACACCAGCTTCGTCGTGAAGGATGAGCTGTGGCTCGTGATGAAGCTGCTGAGCGGCGGCTCTATGCTGGACATCATCAAGTACATCGTGAACCGGGGCGAGCACAAGAACGGCGTGCTGGAAGAGGCCATCATTGCCACCATCCTGAAAGAGGTGCTGGAAGGCCTGGACTACCTGCACCGGAACGGCCAGATCCACAGGGATCTGAAGGCCGGCAACATCCTGCTGGGCGAGGATGGCTCTGTGCAGATCGCCGATTTTGGCGTGTCCGCCTTCCTGGCCACTGGCGGCGACGTGACCAGAAACAAAGTGCGCAAGACCTTCGTGGGCACCCCCTGCTGGATGGCCCCCGAAGTGATGGAACAAGTGCGGGGCTACGACTTCAAGGCCGACATGTGGTCCTTCGGCATCACCGCCATCGAGCTGGCTACAGGCGCCGCTCCCTACCACAAGTACCCCCCCATGAAGGTGCTGATGCTGACCCTGCAGAACGACCCCCCCACCCTGGAAACCGGCGTGGAGGACAAAGAAATGATGAAGAAGTACGGCAAGAGCTTCCGCAAGCTGCTGTCCCTGTGCCTGCAGAAAGATCCCAGCAAGAGGCCCACAGCCGCCGAACTGCTGAAGTGCAAGTTCTTCCAGAAGGCCAAGAACCGCGAGTACCTGATCGAGAAACTGCTGACCCGGACCCCCGATATCGCCCAGCGGGCTAAGAAAGTGCGGAGAGTGCCTGGCAGCTCCGGACATCTGCACAAGACCGAGGACGGCGACTGGGAGTGGAGCGACGACGAGATGGACGAGAAGTCCGAGGAAGGCAAGGCCGCCTTCAGCCAGGAAAAGTCTCGGAGAGTGAAAGAGGAAAACCCCGAGATCGCCGTGTCTGCCAGCACCATCCCTGAGCAGATCCAGAGCCTGAGCGTGCACGATAGCCAGGGCCCTCCCAACGCCAACGAGGACTATAGAGAGGCCAGCAGCTGCGCCGTGAACCTGGTGCTGAGACTGCGGAACAGCCGGAAAGAGCTGAACGACATCAGATTCGAGTTCACCCCCGGCAGAGACACCGCTGATGGGGTGTCCCAGGAACTGTTCAGCGCCGGAGCGGTGGACGGCCACGATGTCGTGATTGTGGCCGCCAATCTGCAGAAAATCGTGGACGACCCCAAGGCCCTGAAAACCCTGACCTTCAAGCTGGCCTCCGGCTGCGACGGCAGCGAGATCCCTGATGAAGTGAAGCTGATCGGCTTCGCCCAGCTGAGCGTGTCCTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMYPYDVPDYAAEP
SGSPVHVQLPQQAAPVTAAAAAAPAAATAAPAPAAPAAPAPAPAPAPAAQ
AVGWPICRDAYELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTS
MDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKY
IVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGE
DGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDF
KADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEM
MKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFFQKAKNREYLIEKLLT
RTPDIAQRAKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAFSQ
EKSRRVKEENPEIAVSASTIPEQIQSLSVHDSQGPPNANEDYREASSCAV
NLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGAVDGHDVVIVAA
NLQKIVDDPKALKTLTFKLASGCDGSEIPDEVKLIGFAQLSVS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48573
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: L493A. Due to ongoing issues with instability in the PAPA box the sequence has been optimised to reduce the secondary structure and repeats present in the n-terminus as the wild-type sequence is extremely difficult to use effectively. The optimised sequence varies greatly from the published sequence though the amino acid sequence is unchanged.; May be grown at 37oC.