cDNA Clone SYNJ1

• Synonyms: INPP5G; PARK20
• Plasmid: pcDNA5D FRT/TO GST SYNJ1 C383S
• Parent Plasmid: pcDNA5D FRT/TO GST
• Expressed: GST SYNJ1 C383S
• Genbank: NM_001160306.1
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCGTTCAGTAAAGGATTCCGGATCTATCACAAATTGGACCCCCCACCTTTCAGCCTCATAGTGGAAACTAGGCATAAGGAAGAATGTCTCATGTTCGAGTCTGGGGCTGTCGCTGTGCTCTCATCTGCAGAAAAAGAGGCAATCAAGGGTACATACTCCAAAGTACTGGATGCATATGGACTCTTAGGTGTTCTGCGGTTAAATCTTGGTGATACTATGTTACATTATCTGGTCCTAGTCACTGGATGTATGTCTGTTGGAAAAATTCAAGAATCTGAAGTTTTCCGAGTTACTTCCACTGAGTTTATATCACTGCGAATCGATTCTTCAGATGAGGATCGCATTTCAGAAGTGCGGAAAGTTTTGAATTCAGGAAACTTTTATTTTGCATGGTCTGCATCTGGCATCAGTTTAGATTTGAGTCTTAATGCGCATCGTAGCATGCAAGAACAGACAACTGATAATAGATTTTTCTGGAATCAGTCTTTGCATTTGCATCTCAAACACTATGGCGTGAATTGTGATGACTGGTTATTACGTCTTATGTGTGGAGGAGTAGAAATCAGAACAATTTATGCTGCTCATAAACAGGCGAAGGCTTGCCTCATTTCAAGATTAAGCTGTGAACGAGCTGGGACCAGGTTTAATGTCCGGGGAACAAATGATGATGGTCATGTTGCCAATTTTGTAGAAACAGAACAGGTTGTGTACTTAGATGACTCAGTTTCTTCCTTCATACAAATCCGAGGATCTGTTCCATTGTTCTGGGAGCAACCAGGGTTGCAAGTGGGATCTCATCGTGTCCGTATGTCAAGGGGATTTGAAGCCAATGCACCTGCTTTTGACAGGCATTTTAGAACACTTAAGAACTTATATGGTAAACAAATAATAGTAAATTTGCTTGGATCTAAGGAAGGTGAACATATGCTAAGTAAAGCTTTCCAGAGTCATTTGAAAGCTTCTGAACATGCTGCTGATATCCAGATGGTGAATTTTGACTATCATCAAATGGTTAAGGGAGGAAAGGCAGAAAAATTACATAGTGTTCTTAAACCTCAAGTCCAGAAGTTTCTAGATTATGGATTTTTTTATTTCAATGGAAGTGAAGTTCAAAGATGCCAGAGTGGTACAGTTCGAACAAACAGCTTGGATTGTCTTGATAGAACAAATAGTGTGCAGGCATTTCTTGGCTTAGAGATGCTAGCTAAACAGTTGGAAGCTCTTGGTTTAGCCGAAAAGCCTCAGTTGGTGACTCGCTTTCAAGAAGTTTTTCGGTCAATGTGGTCCGTGAATGGTGATTCAATCAGTAAGATATATGCAGGAACTGGAGCTCTTGAAGGGAAAGCGAAGGCTGGAAAGTTAAAAGATGGTGCTCGCTCTGTTACCCGAACAATTCAGAATAACTTCTTTGACAGCTCCAAGCAAGAGGCCATTGATGTTTTGCTACTGGGAAATACTCTGAATAGTGATTTAGCTGACAAAGCTCGAGCACTTTTAACTACTGGAAGTTTGCGTGCATCTTCTAAAGTACTAAAGAGCATGTGTGAGAATTTCTACAAATATTCAAAGCCTAAGAAAATTCGAGTATGTGTCGGAACCTGGAATGTGAATGGTGGGAAGCAATTTCGCAGCATAGCTTTTAAGAATCAGACACTCACTGACTGGCTTCTTGATGCACCCAAGTTAGCTGGCATCCAGGAGTTTCAAGATAAAAGAAGTAAGCCAACTGATATATTTGCAATTGGTTTTGAAGAAATGGTAGAATTGAATGCTGGAAACATTGTGAGTGCAAGCACAACAAATCAGAAGCTCTGGGCTGTAGAACTTCAGAAGACAATCTCCAGAGACAACAAGTATGTGCTGCTGGCTTCTGAACAGTTGGTGGGCGTCTGTTTGTTTGTTTTTATCAGACCACAGCATGCTCCTTTTATCAGGGATGTTGCAGTTGATACTGTGAAGACTGGAATGGGAGGTGCAACTGGAAATAAGGGAGCAGTTGCAATCCGAATGCTCTTCCATACAACCAGCCTTTGCTTCGTCTGTAGCCACTTTGCTGCAGGGCAGTCACAAGTCAAAGAAAGAAATGAAGATTTTATAGAAATAGCACGAAAATTGAGTTTTCCTATGGGAAGGATGCTATTTTCCCATGACTATGTATTTTGGTGTGGTGATTTCAACTATCGAATCGATCTCCCTAACGAAGAAGTTAAAGAGCTCATAAGACAGCAAAATTGGGATTCTCTTATAGCAGGAGATCAACTTATCAATCAGAAAAATGCTGGACAGGTTTTTAGAGGATTTTTAGAAGGAAAGGTAACCTTTGCTCCGACATATAAGTATGACTTGTTTTCTGACGACTATGACACCAGTGAAAAGTGTCGCACCCCTGCCTGGACAGACCGTGTCCTTTGGAGAAGGAGGAAATGGCCTTTTGATAGATCAGCTGAAGATCTAGATCTTCTAAATGCTAGTTTTCAAGATGAAAGCAAAATTCTGTACACGTGGACTCCAGGCACTTTGCTGCACTATGGAAGAGCTGAGCTGAAGACTTCTGACCACAGGCCTGTCGTTGCCCTGATTGATATAGATATATTTGAAGTTGAAGCTGAAGAGAGGCAAAACATTTATAAAGAAGTAATTGCAGTTCAGGGTCCACCAGATGGTACAGTATTGGTCTCAATCAAAAGTTCTTTACCAGAAAATAATTTTTTTGATGATGCCTTGATTGATGAGCTTCTGCAGCAGTTTGCAAGTTTTGGTGAAGTTATACTTATAAGATTTGTAGAAGATAAAATGTGGGTTACATTTTTGGAGGGAAGCTCTGCCTTGAATGTTCTGAGCCTAAATGGTAAAGAGTTATTGAATCGGACTATAACTATTGCTTTAAAAAGTCCAGACTGGATCAAAAATTTGGAAGAAGAAATGAGTTTAGAGAAAATTAGCATTGCATTGCCATCATCAACAAGCTCTACCCTGCTTGGTGAAGATGCAGAGGTTGCAGCAGATTTTGATATGGAAGGTGATGTTGATGACTATAGTGCTGAAGTGGAGGAACTTCTTCCTCAGCATCTCCAGCCATCTTCAAGTTCCGGCCTTGGTACTTCCCCCAGCTCTTCACCCCGAACTAGTCCCTGCCAGTCACCTACAATATCAGAGGGTCCTGTACCTTCCCTTCCCATCAGACCAAGCCGAGCACCGTCAAGAACTCCTGGGCCTCCCAGTGCACAGAGTTCTCCTATTGACGCGCAGCCAGCAACGCCGCTGCCGCAGAAAGACCCCGCCCAGCCCTTGGAGCCCAAGCGGCCGCCGCCGCCCCGCCCGGTCGCCCCTCCCACACGCCCGGCTCCCCCACAGAGACCTCCTCCGCCTTCAGGACGCAGTCAGCCTTCACCTCAAGCAGGACTTGCAGGCCCAGGACCTGCTGGATACAGTACAGCCAGACCGACGATTCCTCCTCGTGCTGGAGTTATCAGTGCCCCACAGAGCCACGCGCGGGCATCTGCTGGAAGACTGACTCCTGAAAGCCAAAGCAAAACATCAGAAACGTCGAAAGGTTCAACTTTCCTTCCTGAACCACTGAAGCCTCAGGCTGCTTTTCCTCCGCAGTCTTCTTTGCCCCCGCCTGCTCAAAGGTTGCAAGAGCCTCTTGTCCCTGTGGCAGCACCTATGCCTCAGTCTGGCCCCCAGCCAAATTTGGAAACCCCACCACAACCACCACCTCGAAGCAGGTCATCCCATAGCTTGCCTTCAGAAGCTTCCTCACAACCGCAAGTAAAAACAAATGGAATCTCTGATGGCAAAAGAGAATCACCATTAAAGATTGACCCATTTGAAGATCTGTCATTTAATCTGCTTGCTGTATCAAAGGCTCAGCTATCTGTTCAAACGTCACCTGTTCCCACCCCAGACCCAAAGAGGTTGATTCAGTTGCCTTCTGCAACGCAAAGTAATGTTTTGAGTTCTGTAAGTTGCATGCCAACAATGCCTCCAATTCCAGCTCGGAGTCAATCCCAGGAAAATATGCGAAGTTCTCCAAACCCATTTATTACTGGCTTGACCAGGACAAATCCTTTCAGTGACAGGACTGCTGCTCCTGGAAACCCATTTAGAGCCAAGTCTGAAGAATCAGAGGCAACTTCATGGTTCTCCAAAGAAGAGCCCGTTACTATCAGTCCTTTCCCTTCTCTGCAGCCTCTTGGTCATAACAAAAGCAGGGCTTCATCTTCACTTGATGGCTTTAAGGACAGTTTTGATCTACAGGGCCAGTCTACATTAAAAATTAGCAACCCGAAAGGATGGGTAACCTTCGAGGAAGAAGAGGATTTTGGTGTGAAAGGGAAGTCAAAGTCAGCTTGTTCAGACTTACTGGGTAATCAGCCAAGTTCATTTTCTGGCTCCAACCTGACATTGAATGATGACTGGAATAAAGGTACAAATGTCTCCTTCTGTGTGTTGCCGTCAAGAAGACCTCCTCCACCTCCTGTCCCTCTGCTCCCGCCCGGCACCAGCCCTCCAGTAGATCCTTTCACGACCTTGGCCTCTAAGGCTTCACCCACACTGGACTTTACAGAAAGATAAGTCGAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYSKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRIDSSDEDRISEVRKVLNSGNFYFAWSASGISLDLSLNAHRSMQEQTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVVYLDDSVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKNLYGKQIIVNLLGSKEGEHMLSKAFQSHLKASEHAADIQMVNFDYHQMVKGGKAEKLHSVLKPQVQKFLDYGFFYFNGSEVQRCQSGTVRTNSLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRASSKVLKSMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVSASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFIEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQVFRGFLEGKVTFAPTYKYDLFSDDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQNIYKEVIAVQGPPDGTVLVSIKSSLPENNFFDDALIDELLQQFASFGEVILIRFVEDKMWVTFLEGSSALNVLSLNGKELLNRTITIALKSPDWIKNLEEEMSLEKISIALPSSTSSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTISEGPVPSLPIRPSRAPSRTPGPPSAQSSPIDAQPATPLPQKDPAQPLEPKRPPPPRPVAPPTRPAPPQRPPPPSGRSQPSPQAGLAGPGPAGYSTARPTIPPRAGVISAPQSHARASAGRLTPESQSKTSETSKGSTFLPEPLKPQAAFPPQSSLPPPAQRLQEPLVPVAAPMPQSGPQPNLETPPQPPPRSRSSHSLPSEASSQPQVKTNGISDGKRESPLKIDPFEDLSFNLLAVSKAQLSVQTSPVPTPDPKRLIQLPSATQSNVLSSVSCMPTMPPIPARSQSQENMRSSPNPFITGLTRTNPFSDRTAAPGNPFRAKSEESEATSWFSKEEPVTISPFPSLQPLGHNKSRASSSLDGFKDSFDLQGQSTLKISNPKGWVTFEEEEDFGVKGKSKSACSDLLGNQPSSFSGSNLTLNDDWNKGTNVSFCVLPSRRPPPPPVPLLPPGTSPPVDPFTTLASKASPTLDFTER*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU25880
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence is Insert field is sequence for Isoform D; Silent Mutations D14 (KO internal BamH1), A412, C794; Mutant numbering correct for Isoform D, Full length numbering C422S