cDNA Clone PPIG

• Synonyms: CARS-Cyp, CYP, SCAF10, SRCyp
• Plasmid: pSCB PPIG N699D Bam Not
• Parent Plasmid: pSC-B
• Genbank: Q13427-1 (UniProt ID)
• Species: Human

Sequence of Insert

GGATCCATGGGAATAAAGGTTCAACGTCCTCGATGTTTTTTTGACATTGCCATTAACAATCAACCTGCTGGAAGAGTTGTCTTTGAATTATTTTCTGATGTGTGCCCCAAAACATGCGAGAACTTTCGTTGTCTTTGTACAGGTGAAAAGGGGACCGGGAAATCAACTCAGAAACCATTACATTATAAGAGTTGTCTCTTTCACAGAGTTGTCAAGGATTTTATGGTTCAAGGTGGTGACTTCAGTGAAGGAAATGGACGAGGAGGGGAATCTATCTATGGAGGATTTTTTGAAGACGAGAGTTTCGCTGTTAAACACAACAAAGAATTTCTCTTGTCAATGGCCAACAGAGGGAAGGATACAAATGGTTCACAGTTCTTCATAACAACGAAACCAACTCCTCATTTAGATGGGCATCATGTTGTTTTTGGACAAGTAATCTCTGGTCAAGAAGTTGTAAGAGAGATTGAAAACCAGAAAACAGATGCAGCTAGCAAACCGTTTGCGGAGGTACGGATACTCAGTTGTGGAGAGCTGATTCCCAAATCTAAAGTTAAGAAAGAAGAAAAGAAAAGGCATAAATCATCATCATCTTCCTCCTCCTCATCTAGTGACTCAGATAGCTCAAGTGATTCTCAGTCCTCTTCTGATTCCTCTGATTCCGAAAGTGCTACTGAAGAGAAATCAAAGAAAAGAAAAAAGAAACATCGGAAAAATTCCCGAAAACACAAGAAAGAAAAGAAAAAGCGAAAGAAAAGCAAGAAGAGTGCATCTAGTGAGAGTGAAGCTGAAAATCTTGAAGCACAACCCCAGTCTACTGTCCGTCCAGAAGAGATCCCTCCTATACCTGAAAATAGATTCCTAATGAGAAAAAGTCCTCCTAAAGCTGATGAGAAGGAAAGGAAAAACAGAGAGAGAGAAAGGGAAAGAGAGTGTAATCCACCTAACTCCCAGCCTGCTTCATACCAGAGACGACTTTTAGTTACTAGATCTGGCAGGAAAATTAAAGGAAGAGGACCAAGGCGTTATCGAACTCCTTCCAGATCCAGATCAAGGGATCGTTTCAGACGTAGTGAGACTCCTCCACATTGGAGGCAAGAGATGCAGAGAGCTCAAAGAATGAGGGTATCAAGTGGTGAAAGATGGATCAAGGGGGATAAGAGTGAGTTGAATGAAATAAAAGAAAATCAGAGAAGTCCAGTTAGAGTAAAAGAGAGAAAAATAACAGATCACAGGAATGTATCTGAGAGTCCAAACAGAAAAAATGAAAAGGAGAAGAAAGTTAAAGACCATAAATCTAACAGCAAAGAGAGAGACATCAGAAGAAATTCAGAAAAAGAAGACAAGTATAAAAACAAAGTGAAGAAAAGGGCCAAATCTAAAAGTAGGAGTAAGAGCAAAGAGAAATCAAAGAGTAAAGAAAGAGATTCAAAACATAATAGAAATGAAGAAAAGAGGATGAGGTCAAGGAGTAAAGGAAGGGATCATGAAAATGTTAAAGAAAAAGAAAAGCAGTCTGATTCTAAAGGAAAAGATCAGGAAAGGAGTAGAAGTAAAGAGAAGTCTAAACAGTTAGAATCAAAGAGTAATGAGCATGATCACAGTAAAAGTAAGGAAAAGGATAGACGCGCACAATCCAGGAGTAGAGAATGTGATATAACTAAAGGTAAACACAGTTATAATAGCAGAACAAGAGAACGAAGCAGAAGTAGGGACAGAAGCAGAAGAGTGCGATCAAGAACCCATGACAGAGATCGCAGCAGAAGCAAGGAGTACCATAGATACAGAGAACAGGAATACAGGAGAAGAGGACGGTCACGAAGCCGAGAGAGAAGAACACCACCAGGAAGATCAAGAAGTAAAGATAGGAGGAGAAGGAGGAGAGACTCACGGAGCTCAGAGAGAGAAGAAAGTCAAAGCAGAAACAAAGACAAATACAGAAACCAAGAGAGTAAGAGCTCACACAGAAAAGAAAATTCTGAGAGTGAGAAAAGAATGTACTCTAAAAGTCGTGATCATAATAGCTCAAATAACAGCAGGGAAAAAAAGGCTGATAGAGATCAAAGTCCCTTCTCAAAAATAAAACAAAGCAGTCAGGACGATGAATTAAAGTCCTCCATGTTGAAAAATAAGGAGGATGAGAAGATCAGATCCTCAGTGGAAAAAGAAAACCAAAAATCAAAAGGTCAAGAAAATGACCATGTACATGAAAAAAATAAAAAATTTGATCATGAATCAAGCCCTGGAACAGATGAAGACAAAAGCGGATGAGCGGCCGC

Amino Acid Sequence

MGIKVQRPRCFFDIAINNQPAGRVVFELFSDVCPKTCENFRCLCTGEKGT
GKSTQKPLHYKSCLFHRVVKDFMVQGGDFSEGNGRGGESIYGGFFEDESF
AVKHNKEFLLSMANRGKDTNGSQFFITTKPTPHLDGHHVVFGQVISGQEV
VREIENQKTDAASKPFAEVRILSCGELIPKSKVKKEEKKRHKSSSSSSSS
SSDSDSSSDSQSSSDSSDSESATEEKSKKRKKKHRKNSRKHKKEKKKRKK
SKKSASSESEAENLEAQPQSTVRPEEIPPIPENRFLMRKSPPKADEKERK
NRERERERECNPPNSQPASYQRRLLVTRSGRKIKGRGPRRYRTPSRSRSR
DRFRRSETPPHWRQEMQRAQRMRVSSGERWIKGDKSELNEIKENQRSPVR
VKERKITDHRNVSESPNRKNEKEKKVKDHKSNSKERDIRRNSEKEDKYKN
KVKKRAKSKSRSKSKEKSKSKERDSKHNRNEEKRMRSRSKGRDHENVKEK
EKQSDSKGKDQERSRSKEKSKQLESKSNEHDHSKSKEKDRRAQSRSRECD
ITKGKHSYNSRTRERSRSRDRSRRVRSRTHDRDRSRSKEYHRYREQEYRR
RGRSRSRERRTPPGRSRSKDRRRRRRDSRSSEREESQSRNKDKYRNQESK
SSHRKENSESEKRMYSKSRDHNSSNNSREKKADRDQSPFSKIKQSSQDDE
LKSSMLKNKEDEKIRSSVEKENQKSKGQENDHVHEKNKKFDHESSPGTDE
DKSG*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU48840
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Contains an amino acid change N699D which requires repair. Also contains a silent nucleotide change, T>A 1335 bp.