cDNA Clone PPIG

• Synonyms: CARS-Cyp, CYP, SCAF10, SRCyp
• Plasmid: pCMV5D FLAG PPIG
• Parent Plasmid: pCMV5D FLAG
• Expressed: FLAG-PPIG
• Genbank: NM_004792.2
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGGGAATAAAGGTTCAACGTCCTCGATGTTTTTTTGACATTGCCATTAACAATCAACCTGCTGGAAGAGTTGTCTTTGAATTATTTTCTGATGTGTGCCCCAAAACATGCGAGAACTTTCGTTGTCTTTGTACAGGTGAAAAGGGGACCGGGAAATCAACTCAGAAACCATTACATTATAAGAGTTGTCTCTTTCACAGAGTTGTCAAGGATTTTATGGTTCAAGGTGGTGACTTCAGTGAAGGAAATGGACGAGGAGGGGAATCTATCTATGGAGGATTTTTTGAAGACGAGAGTTTCGCTGTTAAACACAACAAAGAATTTCTCTTGTCAATGGCCAACAGAGGGAAGGATACAAATGGTTCACAGTTCTTCATAACAACGAAACCAACTCCTCATTTAGATGGGCATCATGTTGTTTTTGGACAAGTAATCTCTGGTCAAGAAGTTGTAAGAGAGATTGAAAACCAGAAAACAGATGCAGCTAGCAAACCGTTTGCGGAGGTACGGATACTCAGTTGTGGAGAGCTGATTCCCAAATCTAAAGTTAAGAAAGAAGAAAAGAAAAGGCATAAATCATCATCATCTTCCTCCTCCTCATCTAGTGACTCAGATAGCTCAAGTGATTCTCAGTCCTCTTCTGATTCCTCTGATTCCGAAAGTGCTACTGAAGAGAAATCAAAGAAAAGAAAAAAGAAACATCGGAAAAATTCCCGAAAACACAAGAAAGAAAAGAAAAAGCGAAAGAAAAGCAAGAAGAGTGCATCTAGTGAGAGTGAAGCTGAAAATCTTGAAGCACAACCCCAGTCTACTGTCCGTCCAGAAGAGATCCCTCCTATACCTGAAAATAGATTCCTAATGAGAAAAAGTCCTCCTAAAGCTGATGAGAAGGAAAGGAAAAACAGAGAGAGAGAAAGGGAAAGAGAGTGTAATCCACCTAACTCCCAGCCTGCTTCATACCAGAGACGACTTTTAGTTACTAGATCTGGCAGGAAAATTAAAGGAAGAGGACCAAGGCGTTATCGAACTCCTTCCAGATCCAGATCAAGGGATCGTTTCAGACGTAGTGAGACTCCTCCACATTGGAGGCAAGAGATGCAGAGAGCTCAAAGAATGAGGGTATCAAGTGGTGAAAGATGGATCAAGGGGGATAAGAGTGAGTTGAATGAAATAAAAGAAAATCAGAGAAGTCCAGTTAGAGTAAAAGAGAGAAAAATAACAGATCACAGGAATGTATCTGAGAGTCCAAACAGAAAAAATGAAAAGGAGAAGAAAGTTAAAGACCATAAATCTAACAGCAAAGAGAGAGACATCAGAAGAAATTCAGAAAAAGAAGACAAGTATAAAAACAAAGTGAAGAAAAGGGCCAAATCTAAAAGTAGGAGTAAGAGCAAAGAGAAATCAAAGAGTAAAGAAAGAGATTCAAAACATAATAGAAATGAAGAAAAGAGGATGAGGTCAAGGAGTAAAGGAAGGGATCATGAAAATGTTAAAGAAAAAGAAAAGCAGTCTGATTCTAAAGGAAAAGATCAGGAAAGGAGTAGAAGTAAAGAGAAGTCTAAACAGTTAGAATCAAAGAGTAATGAGCATGATCACAGTAAAAGTAAGGAAAAGGATAGACGCGCACAATCCAGGAGTAGAGAATGTGATATAACTAAAGGTAAACACAGTTATAATAGCAGAACAAGAGAACGAAGCAGAAGTAGGGACAGAAGCAGAAGAGTGCGATCAAGAACCCATGACAGAGATCGCAGCAGAAGCAAGGAGTACCATAGATACAGAGAACAGGAATACAGGAGAAGAGGACGGTCACGAAGCCGAGAGAGAAGAACACCACCAGGAAGATCAAGAAGTAAAGATAGGAGGAGAAGGAGGAGAGACTCACGGAGCTCAGAGAGAGAAGAAAGTCAAAGCAGAAACAAAGACAAATACAGAAACCAAGAGAGTAAGAGCTCACACAGAAAAGAAAATTCTGAGAGTGAGAAAAGAATGTACTCTAAAAGTCGTGATCATAATAGCTCAAATAACAGCAGGGAAAAAAAGGCTGATAGAGATCAAAGTCCCTTCTCAAAAATAAAACAAAGCAGTCAGGACAATGAATTAAAGTCCTCCATGTTGAAAAATAAGGAGGATGAGAAGATCAGATCCTCAGTGGAAAAAGAAAACCAAAAATCAAAAGGTCAAGAAAATGACCATGTACATGAAAAAAATAAAAAATTTGATCATGAATCAAGCCCTGGAACAGATGAAGACAAAAGCGGATGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMGIKVQRPRCFFDIAINNQPAGRVVFELFSDVCPKTCEN
FRCLCTGEKGTGKSTQKPLHYKSCLFHRVVKDFMVQGGDFSEGNGRGGES
IYGGFFEDESFAVKHNKEFLLSMANRGKDTNGSQFFITTKPTPHLDGHHV
VFGQVISGQEVVREIENQKTDAASKPFAEVRILSCGELIPKSKVKKEEKK
RHKSSSSSSSSSSDSDSSSDSQSSSDSSDSESATEEKSKKRKKKHRKNSR
KHKKEKKKRKKSKKSASSESEAENLEAQPQSTVRPEEIPPIPENRFLMRK
SPPKADEKERKNRERERERECNPPNSQPASYQRRLLVTRSGRKIKGRGPR
RYRTPSRSRSRDRFRRSETPPHWRQEMQRAQRMRVSSGERWIKGDKSELN
EIKENQRSPVRVKERKITDHRNVSESPNRKNEKEKKVKDHKSNSKERDIR
RNSEKEDKYKNKVKKRAKSKSRSKSKEKSKSKERDSKHNRNEEKRMRSRS
KGRDHENVKEKEKQSDSKGKDQERSRSKEKSKQLESKSNEHDHSKSKEKD
RRAQSRSRECDITKGKHSYNSRTRERSRSRDRSRRVRSRTHDRDRSRSKE
YHRYREQEYRRRGRSRSRERRTPPGRSRSKDRRRRRRDSRSSEREESQSR
NKDKYRNQESKSSHRKENSESEKRMYSKSRDHNSSNNSREKKADRDQSPF
SKIKQSSQDNELKSSMLKNKEDEKIRSSVEKENQKSKGQENDHVHEKNKK
FDHESSPGTDEDKSG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48914
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Contains a silent nucleotide change, T>A 1335 bp.