cDNA Clone Pol1

• Plasmid: pCE65 (pRS304 Pol1 F58A D62A 12myc)
• Parent Plasmid: pRS304
• Expressed: POL1 F58A D62A
• Species: Yeast

Sequence of Insert

Full sequence of cassette
GCGGCCGCTCTAGAACTAGTGGGATTTGAAAAATAAAAAAGTACCCGTCG
ACCGCAAGTATTCTGATAATGAAGACGATGAATATGACGATGCAGATTTA
CACGGCTTTGAAAAGAAGCAACTGATCAAGAAAGAGTTAGGGGACGACGA
TGAAGATTTATTGATACAGTCGAAAAAATCTCATCAAAAAAAAAAACTAA
AGGAAAAGGAGTTAGAATCATCGATACATGAACTGACCGAAATTGCAGCA
TCCTTACAAAAACGGATACATACGTTAGAAACGGAAAACAAGCTTTTAAA
GAATTTAGTTCTGAGTAGCGGTGAAACGGAAGGAATAAAAAAAGCTGAAA
GCTTAAAGAAGCAAATTTTTGAGAAGGTTCAGAAAGAATAAAATAACAGA
CTATACAGTTTAATTATATATATATATATATATACGTGCATTTTTCTTAA
AGAAATATAACTTTTTTCTTGTACTGCCTGCAATCTCTATTCTTCATTCA
TCACACATCTATTCAAACGCGTTAAAATTTTTTTGTTAGCCTATAAGTAA
AACGCGTCGCGTGTATCCAATTTTACTGAATTAAATTCTTTACTCATTGG
ATGATATATATCTTCTTGGTCTAGACAACGAACACATACCAATTCAGACT
CACGAGAAAAAAAACTGTCTTCACTCTGCTATTAGAAACCAAATAGTATC
AAGGCATAAAATAAACAGTGGAAAATGTCTTCTAAAAGTGAAAAATTAGA
AAAATTGAGAAAGCTGCAAGCCGCTCGAAATGGTACATCCATCGATGATT
ACGAAGGTGACGAATCAGATGGTGACAGAATATACGACGAAATCGACGAA
AAAGAATACAGGGCCAGAAAGCGCCAGGAATTGCTGCATGACGATGCTGT
TGTAGATGCTGATGGTGTAGGCTACGTCGATCGTGGTGTCGAAGAAGATT
GGAGGGAAGTTGATAACAGTTCAAGTGATGAAGACACTGGAAACCTTGCT
AGCAAGGATTCTAAAAGGAAGAAGAATATAAAAAGGGAGAAAGACCACCA
GATTACAGATATGCTGCGAACTCAACATTCCAAATCAACACTGCTAGCAC
ATGCAAAAAAATCCCAAAAAAAAAGTATCCCCATCGATAATTTTGATGAC
ATTCTTGGTGAGTTTGAATCTGGTGAAGTAGAAAAACCCAATATTTTACT
GCCATCCAAATTAAGGGAAAATTTAAATTCTTCGCCGACTAGTGAATTTA
AATCGTCAATAAAGAGAGTTAACGGAAACGATGAGTCAAGTCATGATGCC
GGTATTTCTAAAAAAGTCAAGATTGATCCAGATTCAAGTACTGATAAATA
TTTAGAAATCGAATCATCACCGTTAAAATTACAAAGCAGGAAACTGCGCT
ACGCAAATGATGTACAGGATTTATTGGATGATGTGGAAAATTCCCCAGTA
GTAGCCACAAAGAGACAGAATGTACTTCAAGATACGTTACTAGCAAATCC
ACCATCCGCTCAAAGTTTGGCTGATGAAGAAGATGATGAAGATAGTGATG
AAGATATTATTCTGAAAAGAAGAACTATGAGAAGTGTGACAACAACTAGA
CGCGTGAATATAGACTCAAGAAGTAACCCTTCAACTTCACCGTTTGTCAC
AGCACCCGGAACGCCAATCGGTATTAAAGGACTAACTCCAAGTAAATCTT
TGCAGAGCAACACCGATGTTGCTACACTTGCGGTTAATGTCAAAAAGGAA
GATGTGGTAGATCCGGAAACTGATACTTTCCAAATGTTTTGGTTGGATTA
CTGTGAAGTGAACAACACTTTGATTTTATTTGGTAAGGTTAAACTAAAGG
ATGATAATTGTGTGTCAGCCATGGTTCAAATCAACGGTCTCTGTAGAGAG
CTATTTTTCCTTCCAAGAGAGGGTAAAACACCTACCGATATACACGAGGA
AATAATTCCGTTACTGATGGATAAATATGGACTGGATAATATCCGCGCTA
AACCTCAAAAAATGAAATATTCGTTTGAGCTTCCTGACATTCCATCTGAA
AGTGATTATTTGAAAGTCTTACTTCCATACCAAACACCGAAGTCCAGTCG
AGACACCATTCCATCGGATTTATCTAGCGATACCTTTTATCATGTTTTTG
GTGGGAATTCCAATATTTTTGAAAGTTTTGTTATTCAAAATAGGATTATG
GGACCATGCTGGTTAGATATAAAGGGAGCAGATTTCAATTCAATTCGTAA
TGCTTCTCACTGTGCGGTTGAAGTTTCGGTAGATAAGCCTCAGAATATCA
CGCCTACTACAACAAAGACAATGCCTAATCTGAGATGTCTATCTTTATCG
ATTCAGACGTTAATGAATCCCAAGGAAAATAAGCAAGAGATTGTTTCGAT
AACGTTATCAGCATATAGAAATATCTCGTTGGATTCACCGATACCTGAAA
ATATAAAACCAGACGACTTGTGTACTTTAGTAAGGCCGCCTCAGTCAACT
AGTTTCCCATTAGGTTTAGCTGCACTGGCAAAACAGAAACTACCCGGTAG
AGTCAGGTTGTTCAACAACGAAAAGGCCATGCTATCTTGTTTTTGTGCTA
TGTTAAAAGTGGAAGACCCAGATGTTATCATAGGCCATCGTTTGCAAAAT
GTCTACTTAGATGTTTTAGCTCATAGAATGCATGATCTCAACATTCCAAC
GTTCAGCTCTATTGGCCGTCGTTTAAGAAGGACTTGGCCTGAAAAATTCG
GTAGAGGAAATTCGAATATGAACCATTTTTTTATTAGTGACATTTGCTCT
GGTAGGCTGATATGTGATATCGCCAATGAAATGGGTCAATCATTAACACC
CAAATGTCAAAGTTGGGATCTTTCAGAGATGTATCAAGTTACATGTGAAA
AGGAGCATAAGCCGTTAGATATTGATTATCAAAATCCACAATACCAAAAT
GATGTAAATAGTATGACAATGGCCCTACAAGAGAATATTACTAATTGTAT
GATTTCTGCTGAGGTGTCTTATAGAATTCAATTATTAACCTTGACTAAAC
AGTTGACAAATTTGGCTGGTAATGCATGGGCCCAAACGCTAGGCGGTACA
AGAGCTGGTAGAAATGAGTATATCTTACTACATGAATTTTCAAGAAATGG
TTTTATTGTTCCTGACAAAGAAGGCAATAGAAGTAGAGCTCAGAAACAAA
GACAAAATGAAGAAAATGCTGATGCACCAGTTAATTCTAAAAAGGCAAAA
TATCAGGGTGGTTTAGTTTTTGAACCTGAGAAAGGTCTTCATAAGAATTA
CGTTTTAGTCATGGACTTTAATTCTTTGTATCCATCTATTATCCAGGAAT
TTAATATATGTTTTACCACCGTTGATAGAAATAAGGAAGATATCGATGAG
TTACCAAGCGTACCTCCTTCAGAGGTGGATCAGGGTGTTTTACCAAGGTT
ATTAGCTAATCTGGTGGATCGTCGACGTGAAGTTAAGAAGGTGATGAAAA
CTGAAACTGATCCCCATAAGCGAGTTCAATGTGATATTCGTCAACAAGCT
CTAAAATTGACTGCCAATTCTATGTATGGTTGTTTGGGTTATGTTAACAG
TAGATTTTACGCAAAGCCGCTCGCCATGTTAGTCACTAATAAGGGTCGTG
AGATTTTAATGAATACAAGACAACTAGCTGAAAGTATGAATCTTCTTGTA
GTTTATGGTGATACAGATTCGGTCATGATAGATACCGGTTGTGATAATTA
TGCGGATGCAATTAAAATTGGCTTGGGATTTAAAAGGCTAGTAAATGAGC
GCTATAGATTATTGGAGATTGATATTGATAATGTTTTTAAGAAGTTACTA
TTACATGCCAAGAAAAAGTACGCTGCTTTGACTGTAAATTTGGACAAAAA
TGGTAATGGAACTACTGTTCTAGAAGTTAAAGGGTTGGACATGAAGCGTC
GTGAATTTTGTCCACTTTCGAGGGACGTTTCTATACATGTTTTAAACACC
ATCCTGTCAGATAAGGACCCAGAAGAAGCATTGCAAGAAGTGTATGATTA
CTTAGAAGACATCAGAATAAAAGTGGAAACCAATAACATTAGAATTGATA
AATATAAGATCAATATGAAGCTTTCAAAAGATCCCAAGGCCTACCCAGGT
GGTAAAAACATGCCTGCAGTCCAAGTAGCTCTAAGAATGCGTAAGGCTGG
TAGAGTTGTTAAAGCTGGCTCTGTCATCACTTTTGTGATCACAAAGCAGG
ATGAAATAGACAATGCAGCGGATACGCCGGCTCTTTCTGTGGCTGAACGT
GCCCATGCATTGAATGAGGTAATGATTAAAAGTAACAATTTGATACCTGA
TCCACAATATTATCTCGAGAAACAAATATTCGCGCCGGTAGAAAGGTTGT
TAGAAAGAATTGATAGCTTCAACGTGGTGCGTTTGAGTGAAGCGCTTGGT
TTAGATAGTAAAAAGTATTTTAGAAGAGAGGGCGGTAATAACAATGGAGA
AGATATTAATAATTTGCAACCCTTAGAAACAACAATTACAGACGTTGAAA
GGTTTAAGGATACTGTAACATTAGAATTAAGTTGCCCATCATGCGATAAA
AGGTTTCCATTTGGTGGTATTGTATCTTCAAATTACTATCGCGTGTCATA
TAATGGTTTACAGTGCAAGCATTGTGAGCAACTTTTTACTCCTCTTCAAT
TAACTAGCCAAATAGAGCATTCTATAAGGGCACACATTTCCTTATATTAC
GCAGGGTGGTTACAGTGTGATGACAGCACATGCGGTATAGTTACAAGACA
AGTCTCCGTTTTTGGTAAGCGTTGTTTGAATGACGGCTGCACGGGTGTCA
TGAGATACAAATACAGTGACAAGCAATTGTACAATCAACTTTTATATTTC
GATTCTTTATTCGATTGTGAAAAAAATAAAAAGCAAGAATTGAAGCCAAT
ATATCTACCCGATGATCTCGACTACCCCAAGGAACAGCTGACAGAATCAT
CTATTAAGGCTTTAACAGAACAAAACAGAGAACTAATGGAAACCGGCCGG
AGCGTTGTTCAAAAATATTTGAACGATTGTGGACGTCGCTACGTTGATAT
GACTAGCATATTTGATTTCATGCTAAATCGTACGCTGCAGGTCGACTCCG
GTTCTGCTGCTAGTGGTGAACAAAAGTTGATTTCTGAAGAAGATTTGAAC
GGGGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATT
AATCTCAGAAGAAGACTTGAACGGATCCTCTAGAGGTGAACAAAAGTTGA
TTTCTGAAGAAGATTTGAACGGGGAACAAAAGCTAATCTCCGAGGAAGAC
TTGAACGGTGAACAAAAATTAATCTCAGAAGAAGACTTGAACGGATCCTC
TAGAGGTGAACAAAAGTTGATTTCTGAAGAAGATTTGAACGGGGAACAAA
AGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATTAATCTCAGAA
GAAGACTTGAACGGATCCTCTAGAGGTGAACAAAAGTTGATTTCTGAAGA
AGATTTGAACGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTG
AACAAAAATTAATCTCAGAAGAAGACTTGAACGGATCCACTAGCTAAAAA
GTGGAACGATCATTCAAGAGATCCCCGGGTTAATTAAGGCGCGCCAGATC
TGTTTAGCTTGCCTCGTCCCCGCCGGGTCACCCGGCCAGCGACATGGAGG
CCCAGAATACCCTCCTTGACAGTCTTGACGTGCGCAGCTCAGGGGCATGA
TGTGACTGTCGCCCGTACATTTAGCCCATACATCCCCATGTATAATCATT
TGCATCCATACATTTTGATGGCCGCACGGCGCGAAGCAAAAATTACGGCT
CCTCGCTGCAGACCTGCGAGCAGGGAAACGCTCCCCTCACAGACGCGTTG
AATTGTCCCCACGCCGCGCCCCTGTAGAGAAATATAAAAGGTTAGGATTT
GCCACTGAGGTTCTTCTTTCATATACTTCCTTTTAAAATCTTGCTAGGAT
ACAGTTCTCACATCACATCCGAACATAAACAACCATGGGTAAAAAGCCTG
AACTCACCGCGACGTCTGTCGAGAAGTTTCTGATCGAAAAGTTCGACAGC
GTCTCCGACCTGATGCAGCTCTCGGAGGGCGAAGAATCTCGTGCTTTCAG
CTTCGATGTAGGAGGGCGTGGATATGTCCTGCGGGTAAATAGCTGCGCCG
ATGGTTTCTACAAAGATCGTTATGTTTATCGGCACTTTGCATCGGCCGCG
CTCCCGATTCCGGAAGTGCTTGACATTGGGGAATTCAGCGAGAGCCTGAC
CTATTGCATCTCCCGCCGTGCACAGGGTGTCACGTTGCAAGACCTGCCTG
AAACCGAACTGCCCGCTGTTCTGCAGCCGGTCGCGGAGGCCATGGATGCG
ATCGCTGCGGCCGATCTTAGCCAGACGAGCGGGTTCGGCCCATTCGGACC
GCAAGGAATCGGTCAATACACTACATGGCGTGATTTCATATGCGCGATTG
CTGATCCCCATGTGTATCACTGGCAAACTGTGATGGACGACACCGTCAGT
GCGTCCGTCGCGCAGGCTCTCGATGAGCTGATGCTTTGGGCCGAGGACTG
CCCCGAAGTCCGGCACCTCGTGCACGCGGATTTCGGCTCCAACAATGTCC
TGACGGACAATGGCCGCATAACAGCGGTCATTGACTGGAGCGAGGCGATG
TTCGGGGATTCCCAATACGAGGTCGCCAACATCTTCTTCTGGAGGCCGTG
GTTGGCTTGTATGGAGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGG
AGCTTGCAGGATCGCCGCGGCTCCGGGCGTATATGCTCCGCATTGGTCTT
GACCAACTCTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTG
GGCGCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCG
GGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGT
GTAGAAGTACTCGCCGATAGTGGAAACCGACGCCCCAGCACTCGTCCGAG
GGCAAAGGAATAATCTCGAGTCATGTAATTAGTTATGTCACGCTTACATT
CACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAA
CCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAG
AACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGT
ACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGC
TCGAAGGCTTTAACGAGCTCGAATTCATCGATTACAACTGTACATACGCA
TCCTACTAGATACCTAATGTACAGACATCGGAAGGAGCGCTAGGAAGCTT
TTATTATTGAAGGTTTTCAGCGATATTCTGAGAATGTTTTTTCGGCTCTG
GTGATCAGTTCTCACATGCATCATCCTTTAATAAACCCATGCATTAAGAT
TCGCCTTATCAGCAAGATCAGATAAGCGAACAGCTTCATCAATGAAATAG
AATGCTTAAATCTTGTGAAGCACTGTCATAAGCCAGTTTGATAAAAGCAA
TACCCACAATATAGTTGGATATCATTTACCTTGATCTGCTCTTGCTTTCG
AGGTGACATAGCATTTTTAGCCATCTGATTCCTTAGGTTCAAAGGCACTC
ATCATTCCTCCTCTAAAACACATTTCAACGTCATTCATAATCTGGAATCA
ACATAACAATATCCTAGAACACATCATCAATGGTCACTAACAATGGAGAT
GGTGAGCACCTTGGGATCCGAAGAAACGGAAATTTAAGGCATCCTTCCAA
TAATATGAAAATTCCCAGAAGGGCTCAATCAACAGTTCTCAATTCAAACC
CATTTTATAGTAGGAAGTATTCTATGTCTACCTTAACGCCGAGGGATATA
TGTCGAAGCGTTGATTCTAGCCCCGGGCTGCAGGAATTCGATATCAAGCT
TATCGATACCGTCGACCTCGAGGGGGGGCCCGGTACC

Amino Acid Sequence

MSSKSEKLEKLRKLQAARNGTSIDDYEGDESDGDRIYDEIDEKEYRARKRQELLHDDAVVDADGVGYVDRGVEEDWREVDNSSSDEDTGNLASKDSKRKKNIKREKDHQITDMLRTQHSKSTLLAHAKKSQKKSIPIDNFDDILGEFESGEVEKPNILLPSKLRENLNSSPTSEFKSSIKRVNGNDESSHDAGISKKVKIDPDSSTDKYLEIESSPLKLQSRKLRYANDVQDLLDDVENSPVVATKRQNVLQDTLLANPPSAQSLADEEDDEDSDEDIILKRRTMRSVTTTRRVNIDSRSNPSTSPFVTAPGTPIGIKGLTPSKSLQSNTDVATLAVNVKKEDVVDPETDTFQMFWLDYCEVNNTLILFGKVKLKDDNCVSAMVQINGLCRELFFLPREGKTPTDIHEEIIPLLMDKYGLDNIRAKPQKMKYSFELPDIPSESDYLKVLLPYQTPKSSRDTIPSDLSSDTFYHVFGGNSNIFESFVIQNRIMGPCWLDIKGADFNSIRNASHCAVEVSVDKPQNITPTTTKTMPNLRCLSLSIQTLMNPKENKQEIVSITLSAYRNISLDSPIPENIKPDDLCTLVRPPQSTSFPLGLAALAKQKLPGRVRLFNNEKAMLSCFCAMLKVEDPDVIIGHRLQNVYLDVLAHRMHDLNIPTFSSIGRRLRRTWPEKFGRGNSNMNHFFISDICSGRLICDIANEMGQSLTPKCQSWDLSEMYQVTCEKEHKPLDIDYQNPQYQNDVNSMTMALQENITNCMISAEVSYRIQLLTLTKQLTNLAGNAWAQTLGGTRAGRNEYILLHEFSRNGFIVPDKEGNRSRAQKQRQNEENADAPVNSKKAKYQGGLVFEPEKGLHKNYVLVMDFNSLYPSIIQEFNICFTTVDRNKEDIDELPSVPPSEVDQGVLPRLLANLVDRRREVKKVMKTETDPHKRVQCDIRQQALKLTANSMYGCLGYVNSRFYAKPLAMLVTNKGREILMNTRQLAESMNLLVVYGDTDSVMIDTGCDNYADAIKIGLGFKRLVNERYRLLEIDIDNVFKKLLLHAKKKYAALTVNLDKNGNGTTVLEVKGLDMKRREFCPLSRDVSIHVLNTILSDKDPEEALQEVYDYLEDIRIKVETNNIRIDKYKINMKLSKDPKAYPGGKNMPAVQVALRMRKAGRVVKAGSVITFVITKQDEIDNAADTPALSVAERAHALNEVMIKSNNLIPDPQYYLEKQIFAPVERLLERIDSFNVVRLSEALGLDSKKYFRREGGNNNGEDINNLQPLETTITDVERFKDTVTLELSCPSCDKRFPFGGIVSSNYYRVSYNGLQCKHCEQLFTPLQLTSQIEHSIRAHISLYYAGWLQCDDSTCGIVTRQVSVFGKRCLNDGCTGVMRYKYSDKQLYNQLLYFDSLFDCEKNKKQELKPIYLPDDLDYPKEQLTESSIKALTEQNRELMETGRSVVQKYLNDCGRRYVDMTSIFDFMLNRTLQVDSGSAASGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGSSRGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGSSRGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGSSRGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGSTS*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU26179
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Full POl1 5'UTR Pol1 F58A D62A 12myc 3'UTR cassette can be cut out Not1 Kpn1.; Full sequence of cassette in the insert field.