Plasmid
pX335 USP30 ex5 KO sense
Parent Plasmid
pX335
Expressed
USP30 ex5 KO sense guide + Cas9 D10A
Genbank
NM_032663.3
Species
Human
Vector Type
Mammalian
Antibiotic
Amp
DU Number
DU48922
Unit Source
Location
PPU
MTA
Notes
CRISPR guide plasmid. Targets sense strand of exon 5, designed to KO USP30 when co-transfected with antisense guide 48957. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.
Price
£125.00
To submit an order for this reagent acceptance of the Dundee Material Transfer Agreement is required at checkout.