cDNA Clone RAB1B

• Plasmid: pBABED P U6 RAB1B KO sense
• Parent Plasmid: pBABED P U6
• Expressed: RAB1B KO sense guide
• Genbank: NM_030981.2
• Species: Human

Sequence of Insert

GCTACATCAGCACCATCGGGG

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48932
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR guide plasmid. Targets sense strand of exon 1, designed to KO RAB1B when co-transfected with antisense guide 48946. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.