cDNA Clone USP19

• Plasmid: pcDNA5-FRT/TO-GFP-USP19 (isoform 4) C506A
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP-USP19 C506A
• Genbank: NM_006677.2
• Tag: GFP
• Species: Human

Sequence of Insert

GAATTCATGTCTGGCGGGGCCAGTGCCACAGGCCCAAGGAGAGGGCCCCC
AGGACTGGAGGACACCACTAGTAAGAAGAAGCAGAAGGATCGAGCAAACC
AGGAGAGCAAGGATGGAGATCCTAGGAAAGAGACAGGGTCTCGATATGTT
GCCCAGGCTGGTCTTGAACCTCTGGCCTCAGGTGATCCTTCTGCCTCAGC
CTCCCATGCAGCTGGGATCACAGGCTCACGCCACCGTACCCGGCTGTTCT
TTCCTTCATCGTCAGGGTCAGCATCCACTCCTCAAGAGGAGCAGACCAAA
GAGGGAGCTTGTGAAGACCCTCATGATCTCTTGGCTACTCCCACTCCAGA
GTTGTTGCTCGATTGGAGGCAGAGTGCAGAAGAGGTGATTGTCAAGCTTC
GTGTGGGAGTAGGTCCCCTGCAGCTGGAGGATGTAGATGCTGCTTTCACA
GATACAGACTGTGTGGTGCGGTTTGCAGGTGGTCAGCAGTGGGGTGGTGT
CTTCTATGCTGAGATAAAAAGCTCTTGTGCTAAAGTGCAAACCCGCAAGG
GCAGTCTCCTGCACCTGACACTGCCCAAAAAGGTGCCTATGCTCACGTGG
CCCTCCCTCCTGGTTGAGGCTGATGAACAGCTTTGCATACCACCGCTGAA
CTCCCAAACCTGCCTCCTGGGCTCAGAGGAGAATTTAGCCCCTTTGGCAG
GAGAGAAAGCAGTGCCTCCCGGGAATGACCCAGTCTCTCCAGCCATGGTC
CGGAGCAGAAACCCTGGGAAAGATGACTGTGCCAAGGAGGAGATGGCAGT
GGCAGCAGATGCTGCAACCTTGGTGGATGAGCCCGAGTCGATGGTGAACC
TGGCGTTTGTCAAGAATGACTCGTATGAGAAGGGCCCGGATTCAGTGGTG
GTGCACGTGTACGTGAAGGAGATCTGCAGGGACACCTCAAGAGTACTTTT
CCGTGAGCAGGACTTCACGCTCATCTTCCAGACCAGGGATGGAAACTTCC
TGAGGCTGCACCCGGGCTGTGGGCCCCACACCACCTTCCGTTGGCAGGTG
AAGCTCAGGAATCTGATTGAGCCAGAGCAGTGCACCTTCTGTTTCACGGC
TTCTCGCATCGACATCTGCCTTCGTAAGAGGCAGAGTCAGCGCTGGGGGG
GCCTGGAGGCCCCGGCTGCACGAGTGGGTGGTGCAAAGGTTGCCGTGCCG
ACAGGTCCAACCCCTCTGGATTCAACCCCACCAGGAGGTGCTCCCCACCC
CCTGACAGGCCAGGAGGAGGCCCGGGCTGTGGAGAAGGATAAATCCAAGG
CACGATCTGAGGACACAGGGCTAGACAGTGTGGCAACCCGCACACCCATG
GAGCATGTAACCCCAAAGCCAGAGACACACCTGGCCTCGCCCAAGCCTAC
ATGCATGGTGCCTCCCATGCCCCACAGCCCAGTTAGTGGAGACAGCGTGG
AGGAGGAGGAAGAGGAAGAGAAGAAGGTGTGTCTGCCAGGCTTCACTGGC
CTTGTCAATTTAGGCAACACCgcCTTCATGAACAGCGTCATTCAGTCTCT
GTCCAACACTCGGGAACTCCGGGACTTCTTCCATGACCGCTCCTTTGAGG
CTGAGATCAACTACAACAACCCACTAGGGACTGGTGGGCGTCTGGCCATT
GGCTTTGCCGTGCTGCTTCGGGCGCTGTGGAAGGGCACCCACCATGCCTT
CCAGCCTTCCAAGTTGAAGGCCATTGTGGCGAGTAAGGCCAGCCAGTTCA
CAGGCTATGCACAGCATGATGCCCAGGAGTTCATGGCTTTCCTGCTGGAT
GGGCTGCACGAGGACCTGAATCGCATTCAGAACAAGCCCTACACAGAGAC
CGTGGATTCAGATGGGCGGCCCGATGAGGTGGTAGCTGAGGAAGCATGGC
AGCGGCACAAGATGAGGAATGACTCTTTCATCGTGGACCTATTTCAGGGG
CAGTACAAGTCGAAGCTGGTGTGCCCTGTGTGTGCCAAGGTCTCCATCAC
TTTTGACCCGTTTCTTTATCTGCCGGTGCCCTTGCCACAAAAGCAAAAGG
TTCTCCCTGTCTTTTATTTTGCCCGAGAGCCCCACAGCAAGCCCATCAAG
TTCCTGGTGAGCGTCAGCAAGGAGAACTCCACTGCGAGCGAAGTATTGGA
CTCCCTCTCTCAGAGTGTTCATGTGAAGCCTGAGAACCTGCGTTTGGCGG
AGGTAATTAAGAATCGTTTTCATCGTGTGTTCCTACCCTCCCACTCACTG
GACACTGTGTCCCCATCTGATACGCTCCTCTGCTTTGAGCTGCTATCCTC
AGAGTTGGCTAAGGAGCGGGTAGTGGTGCTAGAGGTGCAACAGCGCCCCC
AGGTGCCCAGCGTCCCCATCTCCAAGTGTGCAGCCTGCCAGCGGAAGCAA
CAGTCGGAGGATGAAAAGCTGAAGCGCTGTACCCGGTGCTACCGTGTGGG
CTACTGCAACCAGCTCTGCCAGAAAACCCACTGGCCTGACCACAAGGGCC
TCTGCCGACCTGAGAACATTGGCTACCCCTTCCTGGTCAGTGTACCTGCC
TCACGCCTCACTTATGCCCGCCTCGCTCAGTTGCTAGAGGGCTATGCCCG
GTACTCTGTGAGTGTATTCCAGCCACCCTTTCAGCCAGGCCGCATGGCCT
TGGAGTCTCAGAGCCCTGGCTGCACCACACTGCTCTCCACAGGTTCCCTG
GAGGCTGGGGACAGCGAGAGAGACCCCATTCAGCCACCTGAGCTCCAGCT
GGTGACCCCTATGGCTGAGGGGGACACAGGGCTTCCCCGGGTGTGGGCAG
CCCCTGACCGGGGTCCTGTGCCCAGCACCAGTGGAATTTCTTCTGAGATG
CTGGCCAGTGGGCCCATTGAGGTTGGCTCCTTGCCAGCTGGCGAGAGGGT
GTCCCGACCCGAAGCTGCTGTGCCTGGGTACCAGCATCCAAGTGAAGCTA
TGAATGCCCACACACCCCAGTTCTTCATCTATAAAATTGATTCATCCAAC
CGAGAGCAGCGGCTAGAGGACAAAGGAGACACCCCACTGGAGCTGGGTGA
CGACTGTAGCCTGGCTCTCGTCTGGCGGAACAATGAGCGCTTGCAGGAGT
TTGTGTTGGTAGCCTCCAAGGAGCTGGAATGTGCTGAGGATCCAGGCTCT
GCCGGTGAGGCTGCCCGGGCCGGCCACTTCACCCTGGACCAGTGCCTCAA
CCTCTTCACACGGCCTGAGGTGCTGGCACCCGAGGAGGCCTGGTACTGCC
CACAGTGCAAACAGCACCGTGAGGCCTCCAAGCAGCTGTTGCTATGGCGC
CTGCCAAATGTTCTCATCGTGCAGCTCAAGCGCTTCTCCTTTCGTAGTTT
TATCTGGCGTGACAAGATCAATGACTTGGTGGAGTTCCCTGTTAGGAACC
TGGACCTGAGCAAGTTCTGCATTGGTCAGAAAGAGGAGCAGCTGCCCAGC
TACGATCTATATGCTGTCATCAACCACTATGGAGGCATGATTGGTGGCCA
CTACACTGCCTGTGCACGCCTGCCCAATGATCGTAGCAGTCAGCGCAGTG
ACGTGGGCTGGCGCTTGTTTGATGACAGCACAGTGACAACGGTAGACGAG
AGCCAGGTTGTGACGCGTTATGCCTATGTACTCTTCTACCGCCGGCGGAA
CTCTCCTGTGGAGAGGCCCCCCAGGGCAGGTCACTCTGAGCACCACCCAG
ACCTAGGCCCTGCAGCTGAGGCTGCTGCCAGCCAGGCTTCCCGGATTTGG
CAGGAGCTGGAGGCTGAGGAGGAGCCGGTGCCTGAGGGGTCTGGGCCCCT
GGGTCCCTGGGGGCCCCAAGACTGGGTGGGCCCCCTACCACGTGGCCCTA
CCACACCAGATGAGGGCTGCCTCCGGTACTTTGTCCTGGGCACCGTGGCG
GCTTTGGTGGCCCTCGTGCTCAACGTGTTCTATCCTCTGGTATCCCAGAG
TCGCTGGAGATGAGTCGAC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSPGEFMS
GGASATGPRRGPPGLEDTTSKKKQKDRANQESKDGDPRKETGSRYVAQAG
LEPLASGDPSASASHAAGITGSRHRTRLFFPSSSGSASTPQEEQTKEGAC
EDPHDLLATPTPELLLDWRQSAEEVIVKLRVGVGPLQLEDVDAAFTDTDC
VVRFAGGQQWGGVFYAEIKSSCAKVQTRKGSLLHLTLPKKVPMLTWPSLL
VEADEQLCIPPLNSQTCLLGSEENLAPLAGEKAVPPGNDPVSPAMVRSRN
PGKDDCAKEEMAVAADAATLVDEPESMVNLAFVKNDSYEKGPDSVVVHVY
VKEICRDTSRVLFREQDFTLIFQTRDGNFLRLHPGCGPHTTFRWQVKLRN
LIEPEQCTFCFTASRIDICLRKRQSQRWGGLEAPAARVGGAKVAVPTGPT
PLDSTPPGGAPHPLTGQEEARAVEKDKSKARSEDTGLDSVATRTPMEHVT
PKPETHLASPKPTCMVPPMPHSPVSGDSVEEEEEEEKKVCLPGFTGLVNL
GNTAFMNSVIQSLSNTRELRDFFHDRSFEAEINYNNPLGTGGRLAIGFAV
LLRALWKGTHHAFQPSKLKAIVASKASQFTGYAQHDAQEFMAFLLDGLHE
DLNRIQNKPYTETVDSDGRPDEVVAEEAWQRHKMRNDSFIVDLFQGQYKS
KLVCPVCAKVSITFDPFLYLPVPLPQKQKVLPVFYFAREPHSKPIKFLVS
VSKENSTASEVLDSLSQSVHVKPENLRLAEVIKNRFHRVFLPSHSLDTVS
PSDTLLCFELLSSELAKERVVVLEVQQRPQVPSVPISKCAACQRKQQSED
EKLKRCTRCYRVGYCNQLCQKTHWPDHKGLCRPENIGYPFLVSVPASRLT
YARLAQLLEGYARYSVSVFQPPFQPGRMALESQSPGCTTLLSTGSLEAGD
SERDPIQPPELQLVTPMAEGDTGLPRVWAAPDRGPVPSTSGISSEMLASG
PIEVGSLPAGERVSRPEAAVPGYQHPSEAMNAHTPQFFIYKIDSSNREQR
LEDKGDTPLELGDDCSLALVWRNNERLQEFVLVASKELECAEDPGSAGEA
ARAGHFTLDQCLNLFTRPEVLAPEEAWYCPQCKQHREASKQLLLWRLPNV
LIVQLKRFSFRSFIWRDKINDLVEFPVRNLDLSKFCIGQKEEQLPSYDLY
AVINHYGGMIGGHYTACARLPNDRSSQRSDVGWRLFDDSTVTTVDESQVV
TRYAYVLFYRRRNSPVERPPRAGHSEHHPDLGPAAEAAASQASRIWQELE
AEEEPVPEGSGPLGPWGPQDWVGPLPRGPTTPDEGCLRYFVLGTVAALVA
LVLNVFYPLVSQSRWR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24838
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 41455. Isoform 4