cDNA Clone SGK1

• Synonyms: SGK
• Plasmid: pX335 SGK1 KO sense B
• Parent Plasmid: pX335
• Expressed: SGK1 KO sense B guide + Cas9 D10A
• Genbank: ENSG00000118515
• Species: Human

Sequence of Insert

gCAGCTTTCATGAAGCAGAGG

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48987
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR guide plasmid. Designed to KO SGK1 when co-transfected with antisense guide 48998. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.