cDNA Clone RAB1B

• Plasmid: pMA RAB1B Nter HA donor
• Parent Plasmid: pMA
• Genbank: NM_030981.2
• Species: Human

Sequence of Insert

gcggccgcAGTTAGGACTGCCCAGTACACGAAGAACCCTGGACAAATGTACTTTTATTAGTCTTACTAGAACCCACATTCTCATTTAATGTTCACAACGTGGGTATTGGGAGGGCAAGGATGACTACCTCCGTCAGACAGATGTGAAACGTGCCAAGCCCAGCCCTAAATTGAGCTTTCTAAACACCGAGTCGCGGTCAGGCTTTCCAGCACTGTCCTCAAGCCTGAGTGCGTCCGGAGTGCAAACTACAAGCCCCAGAAGGCAATGCGCCAGCAGTGATGGGAGTGGGCGCGGAGGCGCGGGCTCCCCGACTACGCCTGCGCAGCGGGGCTGAGTTGGAGAACGTCTTAGACTACAAGTTCCGGGAATCAGCGCGGCGAGGCCTGAGAAGAAGACGTTTGCGCCTGCGCGTCGCGGGCGGGGCCTCTGGGGCGGAGCGGCCACCATCTTGGAACGGGAGGCGGAGCAGAGTCGACTGGGAGCGACCGAGCGGGCCGCCGCCGCCGCCATGTACCCATACGATGTGCCAGATTACGCCAACCCaGAgTAGTGAGTGAGCCCCGCCCGCGCCGTCCAGCGCAGCCTCGATCCCGAATACAttGCTGTACGCTTACCGGGGTTGTCTGGCCCGGGTCAGGACTGTGCGGCGCCCCCACATCCGGGTCCCTCTTCCGCTGACCCCCCCCCACATCCGGGTTCTGCCCCTCCCCCCAGGGGCCCCAGGACCCCGGCTCAAAACCCCGAGCCCTCGCACCCGGGGCCCAGACGTGGCGACCCCCAGGGGCAGCTCTTGGAGCTCGAGACATCGGTCCTACCAGGCCTTATCGGTCAGATGACCCCGCAGTCCCGGTCACTTCAGGCCTCAGTTTCCCCACCTGCTGCTTGACAACTGTCCCGGAGTTTTGGAGCCCGTCTTGATAGTTTCTGGTCTCGGGTTCAGGCTTGGGCCCTTTAGGTCGGGCAGTGAATCCCTCTACCCTTCTCTTTCTCCTGAAGCCCGGCTCCTGGGGCCGGCCCAGAAGCTAGCCAACTACGCGGGAGGCCCCGAAGCGCCGCTTGGGTCGCGTCTCCCGCAGGTCCTCTCTTCCGGGGCTGCCTCCTgcggccgc

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU48991
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR donor plasmid. Designed to KI an HA tag to the N-terminus of RAB1B when co-transfected with guides 48938 and 48952. Donor sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line, contains several silent mutations to prevent binding of guides.