cDNA Clone Sc Top1

• Plasmid: pET28c Sc Top1 1-222
• Parent Plasmid: pET28c
• Expressed: 6His Sc Top1 1-222
• Tag: 6His
• Species: Saccharomyces cerevisiae

Sequence of Insert

CATATGGCTAGCATGACTATTGCTGATGCTTCCAAAGTTAATCATGAGTTGTCTTCTGATGACGATGACGATGTGCCATTATCTCAAACTTTAAAAAAAAGAAAGGTGGCGTCCATGAACTCTGCCTCTCTTCAAGACGAAGCGGAACCTTATGATAGTGATGAGGCAATCTCTAAGATTTCCAAGAAAAAGACTAAGAAAATAAAGACCGAACCAGTGCAATCGTCGTCATTACCATCGCCTCCAGCAAAGAAAAGCGCGACATCAAAGCCTAAAAAAATCAAGAAAGAAGATGGTGATGTAAAGGTAAAAACAACTAAAAAGGAAGAACAGGAGAACGAAAAAAAGAAACGAGAAGAAGAAGAAGAGGAGGACAAGAAAGCGAAGGAGGAGGAGGAAGAATATAAATGGTGGGAAAAAGAAAACGAAGATGACACCATAAAATGGGTCACACTGAAGCATAACGGTGTTATATTCCCTCCACCATACCAGCCCTTACCATCTCACATCAAATTATATTACGATGGGAAGCCAGTAGATTTACCTCCGCAAGCTGAAGAAGTAGCCGGGTTCTTTGCTGCCCTATTAGAGAGTGATCATGCCAAAAATCCTGTTTTCCAAAAGAACTTCTTCAATGATTTCTTGCAAGTACTGAAAGAAAGTGGTGGTCCCCTCAATTAACTCGAG

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTIADASKVNHELSSDDDDDVPLSQTLKKRKVASMNSASLQDEAEPYDSDEAISKISKKKTKKIKTEPVQSSSLPSPPAKKSATSKPKKIKKEDGDVKVKTTKKEEQENEKKKREEEEEEDKKAKEEEEEYKWWEKENEDDTIKWVTLKHNGVIFPPPYQPLPSHIKLYYDGKPVDLPPQAEEVAGFFAALLESDHAKNPVFQKNFFNDFLQVLKESGGPLN*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU47685
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee