cDNA Clone YGL082W

• Plasmid: pGEX6P1 YGL082W Q25N E27P C218S
• Parent Plasmid: pGEX 6P-1
• Expressed: GST YGL082W Q25N E27P C218S
• Genbank: NM_001180947
• Tag: GST

Sequence of Insert

ctcgagATGGATGTTACATTTCTAACCAAAAATGTTCAAATAAATGGCACTCAGTTTAAAATATTACTTCAAAATGGGAACGGTCCATGCGCGCTAATTGCACTAGCTAACGTATTATTGATATCACCTGCCCATGCTCGCTATGCCCAAGAAATTTCCAGACTAGTGAGAGGCAAGGAAACTGTTACTTTAAACGAACTTGTGCAAACTCTAGCCGACATGGGCGTTCAAAATCCGAATGGTACAGATGTGGACAAACAACAGCTACTCCAAATCCTACCACAATTATATAGCGGATTGAACATCAACCCAGAATTTAACGGATCTTTTGAGGACGGCGTAGAAATGTCGATTTTCAGGCTCTACAATGTCGGCATCGTTCATGGATGGATAATAGATGGGGACAATGATCCGAACTCTTACGAACATGTGTCCAAATATTCTTATATGGGCGCGCAAAAAGTTTTGGTACAGTCGTATGAGATCCAAAAGAATAATGCCCAATTTGAAAACAGCGAGCAAATCCAATCTGATGCACCTTACTTAAAATCGTTTCTGGCAAGATCTGCCACACAATTGACTGAATATGGTCTAACTCATCTCAGAGAAATTTTAGTAGAAAGATCGTATGCCGTGCTTTTTAGAAATGATCACTTTAGCACATTATATAAAAACAATGGCGAATTGTTTACTTTGGTCACGGATCCTACTTACAGAAATCGCAAGGACATAAACTGGCAGTCGCTTAAATCCGTGAATGGATCGCAGGATTCTTACTACACGGGCAACTTCATTCCCACAAGTCTGGAAAGAACAGAAACTACTGCTACCGGGCAAAATGAGTCCTACATTTCTAACCCATTCAGTGACCAGAATACAGGGCATGTAACTAGCAACCAAGTTAATAGTGGAGCAAGCGGTGTTCAACAAATCGAAGATGATGAGGAACTAGCTAGGAGGTTACAGGAACAAGAAGATATGCGTGCGGCAAATAACATGCAAAATGGTTATGCTAATAACGGTAGAAATCATCAAAGGGAAAGGTTTGAAAGACCTGAAAAAAATTCAAAGAAAAACAAGTTCCTTCCCTTTAATGGGAGTAATAAAGAAAAGAAAAGAGACAAGTTGAAAAAAAACTGTGTAATTATGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVEMDVTFLTKNVQINGTQFKILLQNGNGPCALIALANVLLISPAHARYAQEISRLVRGKETVTLNELVQTLADMGVQNPNGTDVDKQQLLQILPQLYSGLNINPEFNGSFEDGVEMSIFRLYNVGIVHGWIIDGDNDPNSYEHVSKYSYMGAQKVLVQSYEIQKNNAQFENSEQIQSDAPYLKSFLARSATQLTEYGLTHLREILVERSYAVLFRNDHFSTLYKNNGELFTLVTDPTYRNRKDINWQSLKSVNGSQDSYYTGNFIPTSLERTETTATGQNESYISNPFSDQNTGHVTSNQVNSGASGVQQIEDDEELARRLQEQEDMRAANNMQNGYANNGRNHQRERFERPEKNSKKNKFLPFNGSNKEKKRDKLKKNCVIM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU47807
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please insert point mutation at position 25 from Glutamine to Asparagine in the backbone of construct DU47722.