cDNA Clone RFWD3 (mouse)

• Synonyms: BC027246
• Plasmid: pOA mRFWD3 V639K KI donor
• Parent Plasmid: pOA
• Genbank: ENSMUSG00000033596
• Species: Mouse

Sequence of Insert

gcggccGCTGCAAGTTCAGAGTGGAGGGTGGGAACATGCTGGGAGAACCAGGTAGGGACTGAGGGATGCTAAGAGAACCTGGGGGCCAGGTCTACTTTGATGTGTTAAATGTGCAACTCAGCCACTTGTCCTGGGTTTGAATCCCGGCTCTGTTGAAAGTAGGTGCAGAATATTATATGTATTTCCTTTTTATTTCTCTTACAGACCTGGCTCGTTGCTTTTTAGAGGGTGCAGGAGGATACTTATCTTTCTGTTTGACTTCTGTTGAGTTGGGAATGTGGCCCAGGTCCTCTGGTCATGAGGTCTGGAACAGCCCAAGTGTTGTATTTGGAATGTATGTCATGATTTCCTTTTTTTGCCTTCAGATGTCCTCTGGTATCCCTGTCATATTTACCCAAAGCTGCAGTGGCTGCATTTCCTTATGGTGGGGTGCTGGCTGGAACATTGGAGAATGCCTCCTTCTGGGAGCTAAAATCAGACTTTTCTCATAAGCCTCATGTGCTGTCactcGAGCCAGGtGGaTTcaagGACTTTCAGACAGAGAGCAGCACCCGACACTGTCTTGTcACgTAtAGgCCTGGTGAGTTCTGAAAGTTCTTATGGGCCTGCTATTTTGGGGGTGGGGGGTGGGGGGTGGGGAGGAGGTACAGGTAAGAAGAAACCAGGTGGCTTTGGTCAGCTTCTCTAAGGCTGACAGGCTGTGCTGCAGTTCTTGGTACTACAGTGAAGATGGTGGCTCCCATTTCTTTCACAGAGAGTGCCTCCCTACCCTGTATATTTGACAGCTTTCCTGCCATGTGTTTCTGGAAGTGAATTAAGCACTCTAGAATCTGCTGTATCTAACAACAGTGACAGGATGAGGTGGTCTACCCTTGAGTCCATCTCATCACCTTAGTCCACTTCACCCTCACTTCACCTAAGAAAAGTCCCCAGCTGCAAACAGCCCGATATCAGATCAGTGTTTTAGTTACTCCATATTTAAGTCCAACAAAGTTAAGATGGACAGCATGGTTTTGTGGAACTATCTAGGCAGCTCAACTCTACTGCCTCCAAATCCATGGATAGAACTCCGAGCAGCggccgc

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU54133
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR KI donor designed to introduce a V639K point mutation in exon 11, use in conjunction with guides 52997 and 54005.; Introduces a XhoI site to aid screening.