cDNA Clone VPS13C

• Plasmid: peT15 His SUMO VPS13C (residues 2725-3075)
• Parent Plasmid: peT15 His SUMO
• Expressed: His-SUMO-VPS13C (residues 2725-3075)
• Genbank: Q709C8
• Tag: 6His
• Tag: SUMO

Sequence of Insert

ggatccATGGTCTTTAGTCCCTATTGGTTAATCAACAAGACTACCCGGGTTCTCCAGTATCGTTCAGAAGATATTCATGTGAAACATCCAGCTGATTTCAGGGATATTATTTTATTTTCTTTCAAGAAGAAGAACATTTTTACTAAAAATAAGGTACAATTAAAAATTTCAACCAGTGCCTGGTCCAGTAGTTTCTCATTGGATACAGTGGGAAGTTATGGGTGTGTGAAGTGTCCTGCCAACAATATGGAGTACCTGGTTGGTGTTAGCATCAAAATGAGCAGTTTCAACCTTTCACGAATAGTTACCCTGACTCCCTTTTGTACCATTGCAAACAAGTCATCATTAGAACTAGAAGTTGGCGAGATTGCATCTGATGGCTCAATGCCAACTAATAAATGGAACTATATTGCTTCTTCAGAGTGCCTTCCATTTTGGCCAGAAAGTTTGTCAGGCAAACTTTGTGTGAGAGTGGTGGGCTGTGAAGGATCTTCCAAACCATTCTTTTATAACCGACAGGATAATGGCACTTTATTGAGCTTAGAAGATCTGAATGGGGGTATCTTGGTGGATGTAAACACTGCCGAACATTCAACTGTCATAACTTTTTCTGATTACCATGAGGGATCTGCACCTGCCTTGATAATGAACCATACACCATGGGACATCCTCACATACAAACAGAGTGGGTCACCAGAAGAAATGGTCTTGCTGCCAAGACAGGCTCGACTTTTTGCCTGGGCAGATCCTACTGGTACCAGAAAACTTACATGGACATATGCAGCAAATGTTGGGGAACATGATCTGTTAAAGGATGGATGTGGACAGTTTCCATATGATGCAAACATCCAGATACACTGGGTATCATTTCTGGATGGGCGCCAGAGAGTTTTGCTTTTCACCGATGATGTTGCCTTGGTTTCCAAAGCACTGCAGGCAGAAGAAATGGAACAGGCTGATTATGAAATAACCTTGTCTCTCCACAGTCTTGGGCTTTCACTGGTTAACAATGAAAGCAAGCAGGAAGTTTCCTATATTGGGATAACCAGTTCTGGTGTTGTTTAAgcggccgc

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGMVFSPYWLINKTTRVLQYRSEDIHVKHPADFRDIILFSFKKKNIFTKNKVQLKISTSAWSSSFSLDTVGSYGCVKCPANNMEYLVGVSIKMSSFNLSRIVTLTPFCTIANKSSLELEVGEIASDGSMPTNKWNYIASSECLPFWPESLSGKLCVRVVGCEGSSKPFFYNRQDNGTLLSLEDLNGGILVDVNTAEHSTVITFSDYHEGSAPALIMNHTPWDILTYKQSGSPEEMVLLPRQARLFAWADPTGTRKLTWTYAANVGEHDLLKDGCGQFPYDANIQIHWVSFLDGRQRVLLFTDDVALVSKALQAEEMEQADYEITLSLHSLGLSLVNNESKQEVSYIGITSSGVV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU55366
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Review pilot expression before deciding on antibody
• Low Temp: No