cDNA Clone Tel1 (yeast)

• Plasmid: pMEX3C TEL1 (yeast) 1-222
• Parent Plasmid: pMEX3C
• Expressed: MBP TEL1 (yeast) 1-222
• Genbank: NP_009465.2
• Tag: MBP
• Species: Yeast

Sequence of Insert

GGATCCATGGAGGATCATGGGATTGTAGAAACT*TAAACTTT*TATCATC
AACAAAAATCAAAGAGAGAAACAATGCTTTAGATGAGCTAACAACAATTT
TAAAAGAAGATCCGGAAAGGATACCAACCAAGGCCCTATCTACAACGGCA
GAAGCTTTGGTAGAGTTACTTGCATCTGAACACACAAAATACTGTGACCT
TCTTCGAAACTTGACAGTGTCAACCACAAACAAGCTATCACTTAGTGAGA
ACAGACTCTCCACGATATCGTACGTTTTAAGATTATTTGTAGAAAAATCA
TGTGAGAGATTTAAAGTGAAAACGTTGAAGTTACTTTTAGCAGTAGTACC
TGAATTAATGGTCAAAGATGGTTCCAAAAGTTTATTGGATGCCGTTTCAG
TACATTTATCGTTTGCTTTGGATGCCCTAATTAAAAGTGACCCTTTCAAA
CTGAAATTCATGATACACCAATGGATATCCTTAGTCGATAAAATTTGCGA
GTACTTTCAAAGCCAAATGAAATTATCTATGGTAGACAAAACATTGACCA
ATTTCATATCGATCCTCCTGAATTTATTGGCGTTAGACACAGTTGGTATA
TTTCAAGTGACAAGGACAATTACTTGGACCGTAATAGATTTTTTGAGGCT
CAGCAAAAAAGAAAATGGAAATTAAGCGGCCGC

Amino Acid Sequence

MMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFP
QVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVR
YNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALM
FNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDL
IKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLP
TFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKP
LGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVI
NAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQG
PLGSMEDHGIVETXNFXSSTKIKERNNALDELTTILKEDPERIPTKALST
TAEALVELLASEHTKYCDLLRNLTVSTTNKLSLSENRLSTISYVLRLFVE
KSCERFKVKTLKLLLAVVPELMVKDGSKSLLDAVSVHLSFALDALIKSDP
FKLKFMIHQWISLVDKICEYFQSQMKLSMVDKTLTNFISILLNLLALDTV
GIFQVTRTITWTVIDFLRLSKKENGN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU51937
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please can you make constructs to express the N-terminus of S.cerevisiae Tel1 (1-222) in E. coli. Please then pass this to the protein expression and antibody team. We would like to make affinity purified polyclonal antibodies for this protein in SHEEP.