cDNA Clone PTPN11

• Synonyms: NSA2, BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2, PTPN11, VHL
• Plasmid: pBABED P FLAG VHL a-NSA1 (PTPN11) monobody
• Parent Plasmid: pBABED P FLAG
• Expressed: FLAG-VHL-5xGLY-PTPN11 a-NSA1 monobody
• Tag: FLAG
• Species: Human

Sequence of Insert

VHL
GGATCCGCCATGCCCCGGAGGGCGGAGAACTGGGACGAGGCCGAGGTAGGCGCGGAGGAGGCAGGCGTCGAAGAGTACGGCCCTGAAGAAGACGGCGGGGAGGAGTCGGGCGCCGAGGAGTCCGGCCCGGAAGAGTCCGGCCCGGAGGAACTGGGCGCCGAGGAGGAGATGGAGGCCGGTCGGCCGCGGCCCGTGCTGCGCTCGGTGAACTCGCGCGAGCCCTCCCAGGTCATCTTCTGCAATCGCAGTCCGCGCGTCGTGCTGCCCGTATGGCTCAACTTCGACGGCGAGCCGCAGCCCTACCCAACGCTGCCGCCTGGCACGGGCCGCCGCATCCACAGCTACCGAGGTCACCTTTGGCTCTTCAGAGATGCAGGGACACACGATGGGCTTCTGGTTAACCAAACTGAATTATTTGTGCCATCTCTCAATGTTGACGGACAGCCTATTTTTGCCAATATCACACTGCCAGTGTATACTCTGAAAGAGCGATGCCTCCAGGTTGTCCGGAGCCTAGTCAAGCCTGAGAATTACAGGAGACTGGACATCGTCAGGTCGCTCTACGAAGATCTGGAAGACCACCCAAATGTGCAGAAAGACCTGGAGCGGCTGACACAGGAGCGCATTGCACATCAACGGATGGGAGATGGTGGAGGCGGAGGT

NSA-1
GAATTCGCCATGGTGTCTAGCGTGCCCACCAAGCTGGAAGTGGTGGCCGCTACACCTACCAGCCTGCTGATCTCTTGGGATGCCCCTGCCGTGACCGTGGACTACTACGTGATCACCTATGGCGAGACAGGCAGCGGCGGATATGCCTGGCAAGAGTTTGAAGTGCCCGGCAGCAAGAGCACCGCCACAATCTCTGGACTGAAGCCCGGCGTGGACTATACCATCACAGTGTACGCCGGCTACTACGGCTACCCCACCTACTACAGCAGCCCCATCAGCATCAACTACCGGACCTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSAMPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVW
LNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDL
EDHPNVQKDLERLTQERIAHQRMGDGGGGGEFAMVSSVPTKLEVVAATPTSLLISWDAPAVTVDYYVITYGETGSGGYAWQEFEVPGSKSTATISGLKPG
VDYTITVYAGYYGYPTYYSSPISINYRT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU54843
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Anti-NSA1 (PTPN11) monobody (modified fibronectin III domain) for use in degron work.
• Low Temp: No