cDNA Clone RSF1

• Plasmid: pcDNA5D FRT/TO GFP RSF1 del aa 893-968
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP RSF1 del aa 893-968
• Genbank: NP_057662.3
• Tag: GFP

Sequence of Insert

GGATCCATGGCGACGGCGGCGGCAGCGGCGGCGGTGATGGCTCCTCCGGGCTGCCCGGGTTCGTGCCCCAACTTCGCCGTAGTCTGCTCCTTCTTGGAGCGCTACGGGCCGCTGCTAGACCTGCCTGAGTTGCCGTTCCCTGAGCTGGAGCGGGTGCTGCAGGCGCCGCCGCTGGACGTCGGCAACGGAGAAGTACCAAAAGAATTGGTGGAGCTCCATTTGAAGCTGATGAGGAAAATTGGCAAATCTGTTACTGCAGACAGATGGGAAAAATATTTGATCAAGATATGCCAAGAGTTTAACAGTACCTGGGCATGGGAGATGGAGAAGAAGGGCTATCTTGAAATGAGTGTTGAATGCAAACTAGCACTCTTAAAGTACCTCTGTGAGTGTCAGTTTGATGACAATCTCAAATTCAAGAATATTATTAATGAGGAGGATGCCGATACTATGCGTCTCCAGCCAATTGGTCGAGACAAAGATGGCCTCATGTACTGGTACCAATTGGATCAAGATCACAATGTCAGAATGTACATAGAAGAACAAGATGATCAAGATGGCTCTTCATGGAAATGCATTGTCAGAAATCGAAACGAGTTGGCTGAGACTCTTGCACTCCTGAAAGCACAAATTGATCCTGTACTATTGAAAAACTCTAGCCAACAAGACAACTCTTCTCGGGAAAGTCCCAGCTTAGAGGATGAGGAGACTAAAAAAGAGGAAGAAACACCTAAACAAGAGGAACAGAAAGAAAGTGAAAAGATGAAAAGTGAGGAGCAGCCTATGGATTTAGAAAACCGTTCTACAGCCAATGTTCTAGAAGAGACTACTGTGAAAAAAGAAAAAGAAGATGAAAAGGAACTTGTGAAACTGCCAGTCATAGTGAAGCTAGAAAAACCTTTGCCAGAAAATGAAGAAAAAAAGATTATCAAAGAAGAAAGTGATTCCTTCAAGGAAAATGTCAAACCCATTAAAGTTGAGGTGAAGGAATGTAGAGCAGATCCTAAAGATACCAAAAGTAGCATGGAGAAGCCAGTGGCACAGGAGCCTGAAAGGATCGAATTTGGTGGCAATATTAAATCTTCTCACGAAATTACTGAGAAATCTACTGAAGAAACTGAGAAACTTAAAAATGACCAGCAGGCCAAGATACCACTAAAAAAACGAGAAATTAAACTGAGTGATGATTTTGACAGTCCAGTCAAGGGACCTTTGTGTAAATCAGTTACTCCAACAAAAGAGTTTTTGAAAGATGAAATAAAACAAGAGGAAGAGACTTGTAAAAGGATCTCTACAATCACTGCTTTGGGTCATGAAGGGAAACAGCTGGTAAATGGAGAAGTTAGTGATGAAAGGGTAGCTCCAAATTTTAAGACAGAACCAATAGAGACAAAGTTTTATGAGACAAAGGAAGAGAGCTATAGCCCCTCTAAGGACAGAAATATCATCACGGAGGGAAATGGAACAGAGTCCTTAAATTCTGTCATAACAAGTATGAAAACAGGTGAGCTTGAGAAAGAAACAGCCCCTTTGAGGAAAGATGCAGATAGTTCAATATCAGTCTTAGAGATCCATAGTCAAAAAGCACAAATAGAGGAACCCGATCCTCCAGAAATGGAAACTTCTCTTGATTCTTCTGAGATGGCAAAAGATCTCTCTTCAAAAACTGCTTTATCTTCCACCGAGTCGTGTACCATGAAAGGTGAAGAGAAGTCTCCCAAAACTAAGAAGGATAAGCGCCCACCAATCCTAGAATGTCTTGAAAAGTTAGAGAAGTCCAAAAAGACTTTTCTTGATAAGGACGCACAAAGATTGAGTCCAATACCAGAAGAAGTTCCAAAGAGTACTCTAGAGTCAGAAAAGCCTGGCTCTCCTGAGGCAGCTGAAACTTCTCCACCATCTAATATCATTGACCACTGTGAGAAACTAGCCTCAGAAAAAGAAGTGGTAGAATGCCAGAGTACAAGTACTGTTGGTGGCCAGTCTGTGAAAAAAGTAGACCTAGAAACCCTAAAAGAGGATTCTGAGTTCACAAAGGTAGAAATGGATAATCTGGACAATGCCCAGACCTCTGGCATAGAGGAGCCTTCTGAGACAAAGGGTTCTATGCAAAAAAGCAAATTCAAATATAAGTTGGTTCCTGAAGAAGAAACCACTGCCTCAGAAAATACAGAGATAACCTCTGAAAGGCAGAAAGAGGGCATCAAATTAACAATCAGGATATCAAGTCGGAAAAAGAAGCCCGATTCTCCCCCCAAAGTTCTAGAACCAGAAAACAAGCAAGAGAAGACAGAAAAGGAAGAGGAGAAAACAAATGTGGGTCGTACTTTAAGAAGATCTCCAAGAATATCTAGACCCACTGCAAAAGTGGCTGAGATCAGAGATCAGAAAGCTGATAAAAAAAGAGGGGAAGGAGAAGATGAGGTGGAAGAAGAGTCAACAGCTTTGCAAAAAACTGACAAAAAGGAAATTTTGAAAAAATCAGAGAAAGATACAAATTCTAAAGTAAGCAAGGTAAAACCCAAAGGCAAAGTTCGATGGACTGGTTCTCGGACACGTGGCAGATGGAAATATTCCAGCAATGATGAAAGTGAAGGGTCTGGCAGTGAAAAATCATCTGCAGCTTCAGAAGAGGAGGAAGAAAAGGAAAGTGAAGAAGCCATCCTAGCAGATGATGATGAAGAACGCTTGGTGTATGTTGGTATCAGTATTGAAAACATCATTCCTCCACAAGAGCCAGACTTTTCTGAAGATCAAGAAGAAAAGAAAAAAGATTCAAAAAAATCCAAAGCAAACTTGCTTGAAAGGAGGTCAACAAGAACAAGGAAATGTATAAGCTACAGATTTGATGAGTTTGATGAAGCAATTGATGAAGCTATTGAAGATGACATCAAAGAAGCCGATGGAGGAGGAGTTGGCCNAGGAAAAGATATCTCCACCATCACAGGTCATCGTGGGAAAGACATCTCTACTATTTTGGATGAAGAAAGAAAAGAAAATAAACGACCCCAGAGGGCAGCTGCTGCTCGAAGGAAGAAACGCCGGCGATTAAATGATCTGGACAGTGATAGCAACCTGGATGAAGAAGAGAGCGAGGATGAATTCAAGATCAGTGATGGATCTCAAGATGAGTTTGTTGTGTCTGATGAAAACCCAGATGAAAGTGAAGAAGATCCGCCATCTAATGATGACAGTGACACTGACTTTTGTAGCCGTAGACTGAGGCGACACCCCTCTCGGCCAATGAGGCAGAGCAGGCGTTTGCGAAGAAAGACCCCAAAGAAAAAATATTCCGATGATGATGAAGAGGAGGAATCTGAGGAGAATAGTAGAGACTCTGAAAGTGACTTCAGTGATGATTTTAGTGATGATTTTGTAGAAACTCGGCGAAGGCGGTCAAGGAGAAATCAGAAAAGACAAATTAACTACAAAGAAGACTCAGAAAGTGACGGTTCCCAGAAGAGTTTGCGACGTGGTAAAGAAATAAGGCGAGTACACAAGCGAAGACTTTCCAGCTCAGAGAGTGAAGAGAGCTATTTGTCCAAGAACTCTGAAGATGACGAGCTAGCTAAAGAATCAAAGCGGTCAGTTCGAAAGCGGGGCCGAAGCACAGACGAGTATTCAGAAGCAGATGAGGAGGAGGAGGAAGAGGAAGGCAAACCATCCCGCAAACGGCTGCACCGGATTGAGACGGATGAGGAGGAGAGTTGTGACAATGCTCATGGAGATGCAAATCAGCCTGCCCGTGACAGCCAGCCTAGGGTCCTGCCCTCAGAACAAGAGAGCACCAAGAAGCCCTACCGGATAGAAAGTGATGAGGAAGAGGACTTTGAAAATGTAGGCAAAGTGGGGAGCCCATTGGACTATAGCTTAGTGGACTTACCTTCAACCAATGGACAGAGCCCTGGCAAAGCCATTGAGAACTTGATTGGCAAGCCTACTGAGAAGTCTCAGACCCCCAAGGACAACAGCACAGCCNGTGCAAGCCTAGCCTCCAATGGGACAAGTGGTGGGCAGGAGGCAGGAGCACCAGAAGAGGAGGAAGATGAGCTTTTGAGAGTGACTGACCTTGTTGATTATGTCTGTAACAGTGAACAGTTATAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMATAAAAAAVMAPPGCPGSCPNFAVVCSFLERYGPLLDLPELPFPELERVLQAPPLDVGNGEVPKELVELHLKLMRKIGKSVTADRWEKYLIKICQEFNSTWAWEMEKKGYLEMSVECKLALLKYLCECQFDDNLKFKNIINEEDADTMRLQPIGRDKDGLMYWYQLDQDHNVRMYIEEQDDQDGSSWKCIVRNRNELAETLALLKAQIDPVLLKNSSQQDNSSRESPSLEDEETKKEEETPKQEEQKESEKMKSEEQPMDLENRSTANVLEETTVKKEKEDEKELVKLPVIVKLEKPLPENEEKKIIKEESDSFKENVKPIKVEVKECRADPKDTKSSMEKPVAQEPERIEFGGNIKSSHEITEKSTEETEKLKNDQQAKIPLKKREIKLSDDFDSPVKGPLCKSVTPTKEFLKDEIKQEEETCKRISTITALGHEGKQLVNGEVSDERVAPNFKTEPIETKFYETKEESYSPSKDRNIITEGNGTESLNSVITSMKTGELEKETAPLRKDADSSISVLEIHSQKAQIEEPDPPEMETSLDSSEMAKDLSSKTALSSTESCTMKGEEKSPKTKKDKRPPILECLEKLEKSKKTFLDKDAQRLSPIPEEVPKSTLESEKPGSPEAAETSPPSNIIDHCEKLASEKEVVECQSTSTVGGQSVKKVDLETLKEDSEFTKVEMDNLDNAQTSGIEEPSETKGSMQKSKFKYKLVPEEETTASENTEITSERQKEGIKLTIRISSRKKKPDSPPKVLEPENKQEKTEKEEEKTNVGRTLRRSPRISRPTAKVAEIRDQKADKKRGEGEDEVEEESTALQKTDKKEILKKSEKDTNSKVSKVKPKGKVRWTGSRTRGRWKYSSNDESEGSGSEKSSAASEEEEEKESEEAILADDDEERLVYVGISIENIIPPQEPDFSEDQEEKKKDSKKSKANLLERRSTRTRKCISYRFDEFDEAIDEAIEDDIKEADGGGVGXGKDISTITGHRGKDISTILDEERKENKRPQRAAAARRKKRRRLNDLDSDSNLDEEESEDEFKISDGSQDEFVVSDENPDESEEDPPSNDDSDTDFCSRRLRRHPSRPMRQSRRLRRKTPKKKYSDDDEEEESEENSRDSESDFSDDFSDDFVETRRRRSRRNQKRQINYKEDSESDGSQKSLRRGKEIRRVHKRRLSSSESEESYLSKNSEDDELAKESKRSVRKRGRSTDEYSEADEEEEEEEGKPSRKRLHRIETDEEESCDNAHGDANQPARDSQPRVLPSEQESTKKPYRIESDEEEDFENVGKVGSPLDYSLVDLPSTNGQSPGKAIENLIGKPTEKSQTPKDNSTAXASLASNGTSGGQEAGAPEEEEDELLRVTDLVDYVCNSEQL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU55864
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: HI, Could you please make the RSF1 clone with residues 893-968 deleted. Thanks!; taken on from Simone 25/5/17, taken back 13/06/17; ; silent changes 2679 A-G, 3783 T-C, and 3900 A-G
• Low Temp: No