cDNA Clone Shroom 4

• Plasmid: pcDNA FRT TO GFP Shroom 4
• Parent Plasmid: pcDNA FRT TO GFP
• Expressed: GFP Shroom 4
• Tag: GFP

Sequence of Insert

GAATTCATGGAGAACCGGCCTGGGTCCTTCCAGTACGTCCCTGTGCAGCTGCAAGGGGGGGCACCCTGGGGCTTCACCCTTAAGGGGGGTCTGGAACACTGTGAGCCGCTCACAGTGTCTAAGATTGAAGATGGAGGCAAGGCAGCTTTGTCCCAGAAGATGAGGACTGGTGATGAGCTGGTGAATATCAATGGCACTCCATTATATGGCTCCCGCCAAGAGGCCCTCATTCTCATCAAAGGCTCCTTCCGGATTCTCAAGCTGATTGTCAGGAGGAGGAACGCCCCTGTCAGTAGGCCGCACTCATGGCATGTGGCCAAGCTGCTGGAGGGATGCCCTGAAGCAGCCACCACCATGCATTTCCCTTCTGAAGCCTTCAGCTTGTCCTGGCATTCTGGCTGCAACACAAGTGACGTGTGTGTGCAGTGGTGTCCACTCTCCCGGCATTGCAGCACCGAGAAAAGCAGCTCCATTGGCAGCATGGAGAGCCTGGAGCAACCAGGCCAAGCCACCTATGAGAGCCATCTGTTGCCTATTGACCAGAACATGTACCCTAACCAGCGTGACTCAGCCTACAGCTCCTTCTCGGCCAGCTCAAATGCTTCTGACTGTGCCCTTTCCCTCAGGCCAGAGGAGCCAGCCTCTACAGACTGCATCATGCAAGGCCCAGGGCCAACTAAGGCCCCCAGTGGCCGGCCTAATGTGGCTGAGACCTCAGGAGGTAGTCGGCGCACCAATGGGGGCCACCTGACCCCCAGCTCTCAGATGTCATCCCGTCCACAGGAGGGATACCAGTCAGGGCCCGCCAAAGCAGTCAGGGGCCCACCACAACCTCCAGTGAGGCGGGACAGCCTTCAGGCCTCCAGAGCCCAACTCCTCAATGGAGAGCAGCGCAGGGCATCTGAGCCTGTGGTCCCCTTGCCACAGAAGGAGAAACTGAGCTTAGAGCCTGTGCTACCCGCAAGGAACCCTAATAGGTTCTGTTGCCTCAGTGGGCATGACCAAGTGACAAGTGAGGGCCATCAGAACTGTGAGTTCAGTCAGCCTCCTGAATCCAGCCAACAGGGCTCTGAGCATCTACTGATGCAGGCCTCAACCAAAGCTGTTGGATCCCCAAAAGCCTGTGACAGAGCTTCCAGCGTGGATTCCAACCCACTCAATGAGGCTTCTGCAGAGCTAGCTAAGGCTTCTTTTGGCAGACCTCCACATCTCATAGGACCCACAGGGCATCGCCATAGTGCCCCTGAACAGCTGCTGGCATCCCACCTGCAGCATGTGCACCTTGATACCAGGGGCAGCAAAGGGATGGAGCTCCCACCCGTACAGGATGGGCACCAGTGGACTCTGTCCCCTTTGCACAGCAGCCACAAAGGGAAGAAAAGTCCATGCCCCCCTACAGGAGGAACCCATGACCAGTCCAGCAAAGAAAGAAAGACCAGACAAGTGGATGACAGGTCTTTAGTTTTGGGACACCAGAGCCAAAGCAGTCCCCCACATGGAGAGGCTGATGGACACCCCTCAGAAAAAGGTTTCCTGGACCCAAACAGAACAAGCAGAGCAGCCAGTGAATTGGCCAACCAGCAACCCTCTGCCTCTGGCTCCCTTGTTCAACAAGCCACGGACTGTTCTTCAACCACTAAAGCAGCTAGTGGCACAGAGGCAGGTGAAGAAGGGGACAGCGAGCCCAAGGAGTGCAGCCGGATGGGTGGTAGGCGAAGTGGAGGGACCCGGGGCCGCTCGATCCAAAACCGGCGGAAGAGTGAGCGTTTTGCTACCAATCTGCGTAATGAAATTCAGAGGAGGAAGGCCCAGCTCCAGAAAAGCAAGGGTCCCTTGTCACAGCTGTGTGACACTAAGGAGCCAGTGGAAGAGACCCAGGAGCCCCCAGAAAGTCCTCCACTCACTGCCTCTAACACATCTCTTCTATCTTCATGTAAAAAACCTCCCAGCCCCAGAGACAAGCTCTTCAACAAAAGCATGATGCTCAGAGCTAGGTCTTCCGAGTGCCTCAGCCAAGCCCCTGAGAGCCATGAATCTAGGACAGGCTTAGAGGGACGAATAAGCCCTGGCCAGAGGCCTGGCCAGTCCTCTTTGGGCCTGAACACCTGGTGGAAAGCACCTGACCCATCCTCCTCAGACCCTGAGAAAGCACATGCTCACTGTGGAGTCCGTGGAGGTCATTGGAGATGGTCTCCAGAGCATAATTCACAGCCACTTGTGGCAGCAGCCATGGAAGGCCCTTCCAACCCAGGTGACAACAAGGAATTGAAGGCTTCTACTGCTCAAGCTGGGGAGGATGCCATCCTCTTGCCTTTTGCAGACAGAAGAAAGTTCTTTGAAGAGAGTAGCAAATCCTTATCTACATCTCATTTGCCAGGTTTAACCACTCATAGCAACAAGACTTTTACCCAGAGACCAAAACCTATAGACCAAAACTTCCAGCCAATGAGCTCCAGCTGTAGGGAATTGAGGCGCCATCCCATGGACCAATCATATCATTCCGCAGACCAACCATATCATGCCACAGACCAATCATATCATTCCATGTCACCCCTTCAGTCAGAAACTCCCACTTACTCAGAATGTTTTGCAAGCAAAGGTCTAGAAAATTCCATGTGTTGTAAGCCACTACACTGTGGTGATTTTGATTACCACAGGACCTGCTCTTACTCCTGCAGTGTTCAAGGAGCTCTAGTCCATGATCCTTGCATTTATTGTTCTGGGGAAATCTGCCCTGCCTTGCTAAAGAGAAATATGATGCCAAATTGCTACAACTGCCGGTGCCACCACCACCAATGCATTCGGTGTTCAGTTTGCTATCATAATCCTCAGCACAGTGCCCTCGAGGACAGCAGCTTGGCACCTGGCAACACTTGGAAACCCAGGAAGCTGACAGTGCAGGAATTTCCTGGGGACAAATGGAATCCAATAACAGGAAACAGGAAGACCAGCCAGTCAGGGAGGGAAATGGCTCATTCCAAGACTAGCTTTTCATGGGCAACCCCTTTCCATCCTTGCCTTGAGAACCCAGCACTGGACTTGTCAAGCTACCGAGCAATTTCTTCTCTTGACCTCCTTGGAGACTTCAAACATGCTTTGAAAAAATCAGAGGAAACTTCAGTTTATGAGGAGGGGAGCTCCCTTGCCTCCATGCCCCACCCACTGCGCAGCCGTGCCTTCTCAGAGAGTCACATCAGCTTGGCGCCCCAAAGCACCCGGGCCTGGGGGCAGCATAGGAGGGAGCTCTTTAGCAAAGGTGATGAGACCCAGTCGGATCTTCTCGGAGCCAGGAAGAAGGCCTTTCCTCCTCCTCGCCCTCCTCCTCCCAACTGGGAGAAGTACAGGCTCTTTCGTGCAGCCCAGCAGCAGAAGCAGCAACAGCAGCAGCAACAGCAGCAGCAGAAGCAACAGGAGGAGGAGGAGGAGGAGGAGGAAGAAGAAGAAGAAGAGGAAGAGGAAGAGGAGGAGGAGGAGGCAGAGGAGGAGGAGGAGGAGCTGCCACCCCAGTATTTCAGTTCAGAAACCTCTGGTTCCTGTGCTCTCAATCCTGAGGAGGTCCTAGAGCAGCCACAACCCCTCAGCTTTGGCCACCTGGAGGGCTCGAGACAGGGTTCACAAAGTGTCCCAGCAGAGCAAGAATCCTTTGCACTCCATTCCAGTGATTTCTTGCCTCCAATAAGGGGTCACTTGGGATCTCAACCTGAGCAGGCTCAGCCCCCTTGCTACTATGGCATTGGTGGGCTTTGGAGGACATCGGGACAGGAAGCCACTGAATCCGCCAAACAAGAGTTTCAGCACTTTTCGCCTCCTTCAGGGGCCCCAGGAATCCCTACCTCTTACTCAGCTTATTACAATATTTCTGTGGCCAAGGCAGAGCTGCTGAACAAACTGAAAGACCAACCTGAGATGGCAGAGATTGGCCTAGGAGAGGAGGAAGTTGACCATGAACTGGCTCAAAAAAAGATACAGCTTATCGAAAGCATCAGCAGAAAACTTTCTGTCTTGCGGGAGGCCCAGCGAGGGCTGCTAGAGGACATCAATGCCAATTCTGCCCTTGGGGAGGAGGTGGAGGCCAACTTAAAAGCCGTCTGCAAATCCAATGAATTTGAAAAGTACCACTTGTTTGTTGGGGACCTGGACAAAGTGGTCAACCTGTTGCTGTCACTCTCTGGACGACTGGCCCGGGTGGAGAATGCTCTGAACAGCATCGATTCAGAGGCCAACCAGGAGAAGTTGGTACTGATAGAGAAGAAGCAGCAGCTGACGGGGCAGTTGGCAGATGCCAAGGAGCTGAAGGAGCACGTGGACCGCCGGGAGAAGTTGGTGTTTGGCATGGTCTCCCGCTACCTGCCTCAGGACCAGCTCCAAGATTACCAGCACTTTGTCAAGATGAAATCTGCTCTCATCATTGAACAGCGAGAGCTGGAGGAGAAGATCAAGCTCGGGGAAGAGCAACTCAAATGTCTCAGGGAGAGTCTACTCCTGGGGCCCAGCAATTTCTAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSPGEFMENRPGSFQYVPVQLQGGAPWGFTLKGGLEHCEPLTVSKIEDGGKAALSQKMRTGDELVNINGTPLYGSRQEALILIKGSFRILKLIVRRRNAPVSRPHSWHVAKLLEGCPEAATTMHFPSEAFSLSWHSGCNTSDVCVQWCPLSRHCSTEKSSSIGSMESLEQPGQATYESHLLPIDQNMYPNQRDSAYSSFSASSNASDCALSLRPEEPASTDCIMQGPGPTKAPSGRPNVAETSGGSRRTNGGHLTPSSQMSSRPQEGYQSGPAKAVRGPPQPPVRRDSLQASRAQLLNGEQRRASEPVVPLPQKEKLSLEPVLPARNPNRFCCLSGHDQVTSEGHQNCEFSQPPESSQQGSEHLLMQASTKAVGSPKACDRASSVDSNPLNEASAELAKASFGRPPHLIGPTGHRHSAPEQLLASHLQHVHLDTRGSKGMELPPVQDGHQWTLSPLHSSHKGKKSPCPPTGGTHDQSSKERKTRQVDDRSLVLGHQSQSSPPHGEADGHPSEKGFLDPNRTSRAASELANQQPSASGSLVQQATDCSSTTKAASGTEAGEEGDSEPKECSRMGGRRSGGTRGRSIQNRRKSERFATNLRNEIQRRKAQLQKSKGPLSQLCDTKEPVEETQEPPESPPLTASNTSLLSSCKKPPSPRDKLFNKSMMLRARSSECLSQAPESHESRTGLEGRISPGQRPGQSSLGLNTWWKAPDPSSSDPEKAHAHCGVRGGHWRWSPEHNSQPLVAAAMEGPSNPGDNKELKASTAQAGEDAILLPFADRRKFFEESSKSLSTSHLPGLTTHSNKTFTQRPKPIDQNFQPMSSSCRELRRHPMDQSYHSADQPYHATDQSYHSMSPLQSETPTYSECFASKGLENSMCCKPLHCGDFDYHRTCSYSCSVQGALVHDPCIYCSGEICPALLKRNMMPNCYNCRCHHHQCIRCSVCYHNPQHSALEDSSLAPGNTWKPRKLTVQEFPGDKWNPITGNRKTSQSGREMAHSKTSFSWATPFHPCLENPALDLSSYRAISSLDLLGDFKHALKKSEETSVYEEGSSLASMPHPLRSRAFSESHISLAPQSTRAWGQHRRELFSKGDETQSDLLGARKKAFPPPRPPPPNWEKYRLFRAAQQQKQQQQQQQQQQKQQEEEEEEEEEEEEEEEEEEEEEAEEEEEELPPQYFSSETSGSCALNPEEVLEQPQPLSFGHLEGSRQGSQSVPAEQESFALHSSDFLPPIRGHLGSQPEQAQPPCYYGIGGLWRTSGQEATESAKQEFQHFSPPSGAPGIPTSYSAYYNISVAKAELLNKLKDQPEMAEIGLGEEEVDHELAQKKIQLIESISRKLSVLREAQRGLLEDINANSALGEEVEANLKAVCKSNEFEKYHLFVGDLDKVVNLLLSLSGRLARVENALNSIDSEANQEKLVLIEKKQQLTGQLADAKELKEHVDRREKLVFGMVSRYLPQDQLQDYQHFVKMKSALIIEQRELEEKIKLGEEQLKCLRESLLLGPSNF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU59055
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: pcDNA5/FRT/TO vector please - expressing GFP tagged Shroom4 https://www.ncbi.nlm.nih.gov/gene/57477 in the N terminus, NOTE extra 4 Qs from 1123-1126 compared to reference, extra E 1136, silent change 3468 A-G
• Low Temp: No