cDNA Clone Rbx1

• Plasmid: pFBDual GST-Cullin2, 6His-Rbx1
• Parent Plasmid: pFastbacDual 6P1
• Expressed: 6His-Rbx1
• Genbank: NM_014248.2
• Tag: GST
• Species: Human

Sequence of Insert

gctagcATGCATCATCACCATCACCATATGGCGGCAGCGATGGATGTGGA
TACCCCGAGCGGCACCAACAGCGGCGCGGGCAAGAAGCGCTTTGAAGTGA
AAAAGTGGAATGCAGTAGCCCTCTGGGCCTGGGATATTGTGGTTGATAAC
TGTGCCATCTGCAGGAACCACATTATGGATCTTTGCATAGAATGTCAAGC
TAACCAGGCGTCCGCTACTTCAGAAGAGTGTACTGTCGCATGGGGAGTCT
GTAACCATGCTTTTCACTTCCACTGCATCTCTCGCTGGCTCAAAACACGA
CAGGTGTGTCCATTGGACAACAGAGAGTGGGAATTCCAAAAGTATGGGCA
CTAGggtacc

Amino Acid Sequence

MHHHHHHMAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCA
ICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQV
CPLDNREWEFQKYGH*

• Vector Type: Baculovirus
• Antibiotic: Amp
• Full Snapgene Map: pFBDual GST-Cullin2, 6His-Rbx1203.99 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU21546
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: SC20032 (Cullin2) SC20946 (6HIS-Rbx1); (Cullin2 was subcloned BamHI-Not1 from SC20032 into the PPH driven cassette of SC20978 (pFBDual-GST6P-1 derived) thereby replacing the Cullin3 coding region). 6HIS-Rbx1 was previously subcloned Nhe1-Kpn1into the PP10 driven cassette.
• Low Temp: No