cDNA Clone SCYL2

• Synonyms: CVAK104
• Plasmid: pcDNA5D FRT/TO GFP SCYL2
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP SCYL2
• Genbank: NM_017988.5
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGAGTCCATGCTTAATAAATTGAAGAGTACTGTTACAAAAGTAACAGCTGATGTCACTAGTGCTGTAATGGGAAATCCTGTCACTAGAGAATTTGATGTTGGTCGACACATTGCCAGTGGTGGCAATGGGCTAGCTTGGAAGATTTTTAATGGCACAAAAAAGTCAACAAAGCAGGAAGTGGCAGTTTTTGTCTTTGATAAAAAACTGATTGACAAGTATCAAAAATTTGAAAAGGATCAAATCATTGATTCTCTAAAACGAGGAGTCCAACAGTTAACTCGGCTTCGACACCCTCGACTTCTTACTGTCCAGCATCCTTTAGAAGAATCCAGGGATTGCTTGGCATTTTGTACAGAACCAGTTTTTGCCAGTTTAGCCAATGTTCTTGGTAACTGGGAAAATCTACCTTCCCCTATATCTCCAGACATTAAGGATTATAAACTTTATGATGTAGAAACCAAATATGGTTTGCTTCAGGTTTCTGAAGGATTGTCATTCTTGCATAGCAGTGTGAAAATGGTGCATGGAAATATCACTCCTGAAAATATAATTTTGAATAAAAGTGGAGCCTGGAAAATAATGGGTTTTGATTTTTGTGTATCATCAACCAATCCTTCTGAACAAGAGCCTAAATTTCCTTGTAAAGAATGGGACCCAAATTTACCTTCATTGTGTCTTCCAAATCCTGAATATTTGGCTCCTGAATACATACTTTCTGTGAGCTGTGAAACAGCCAGTGATATGTATTCTTTAGGAACTGTTATGTATGCTGTATTTAATAAAGGGAAACCTATATTTGAAGTCAACAAGCAAGATATTTACAAGAGTTTCAGTAGGCAGTTGGATCAGTTGAGTCGTTTAGGATCTAGTTCACTTACAAATATACCTGAGGAAGTTCGTGAACATGTAAAGCTACTGTTAAATGTAACTCCGACTGTAAGACCAGATGCAGATCAAATGACAAAGATTCCCTTCTTTGATGATGTTGGTGCAGTAACACTGCAATATTTTGATACCTTATTCCAAAGAGATAATCTTCAGAAATCACAGTTTTTCAAAGGACTGCCAAAGGTTCTACCAAAACTGCCCAAGCGTGTCATTGTGCAGAGAATTTTGCCTTGTTTGACTTCAGAATTTGTAAACCCTGACATGGTACCTTTTGTTTTGCCCAATGTTTTACTTATTGCTGAGGAATGCACCAAAGAAGAATATGTCAAATTAATTCTTCCTGAACTTGGCCCTGTGTTTAAGCAGCAGGAGCCAATCCAGATTTTGTTAATTTTCCTACAAAAAATGGATTTGCTACTAACCAAAACCCCTCCTGATGAGATAAAGAACAGTGTTCTACCCATGGTTTACAGAGCACTAGAAGCTCCTTCCATTCAGATCCAGGAGCTCTGTCTAAACATCATTCCAACCTTTGCAAATCTTATAGACTACCCATCCATGAAAAACGCTTTGATACCAAGAATTAAAAATGCTTGTCTACAAACATCTTCCCTTGCGGTTCGTGTAAATTCATTAGTGTGCTTAGGAAAGATTTTGGAATACTTGGATAAGTGGTTTGTACTTGATGATATCCTACCCTTCTTACAACAAATTCCATCCAAGGAACCTGCGGTCCTCATGGGAATTTTAGGTATTTACAAATGTACTTTTACTCATAAGAAGTTGGGAATCACCAAAGAGCAGCTGGCCGGAAAAGTGTTGCCTCATCTTATTCCCCTGAGTATTGAAAACAATCTTAATCTTAATCAGTTCAATTCTTTCATTTCCGTCATAAAAGAAATGCTTAATAGATTGGAGTCTGAACATAAGACTAAACTGGAACAACTTCATATAATGCAAGAACAGCAGAAATCTTTGGATATAGGAAATCAAATGAATGTTTCTGAGGAGATGAAAGTTACAAATATTGGGAATCAGCAAATTGACAAAGTTTTTAACAACATTGGAGCAGACCTTCTGACTGGCAGTGAGTCCGAAAATAAAGAGGACGGGTTACAGAATAAACATAAAAGAGCATCACTTACACTTGAAGAAAAACAAAAATTAGCAAAAGAACAAGAGCAGGCACAGAAGCTGAAAAGCCAGCAGCCTCTTAAACCCCAAGTGCACACACCTGTTGCTACTGTTAAACAGACTAAGGACTTGACAGACACATTGATGGATAATATGTCATCCTTGACCAGCCTTTCTGTTAGTACCCCTAAATCTTCTGCTTCAAGTACTTTCACTTCTGTTCCTTCCATGGGCATTGGTATGATGTTTTCTACACCAACTGATAATACAAAGAGAAATTTGACAAATGGCCTAAATGCCAATATGGGCTTTCAGACTTCAGGATTCAACATGCCCGTTAATACAAACCAGAACTTCTACAGTAGTCCAAGCACAGTTGGAGTGACCAAGATGACTCTGGGAACACCTCCCACTTTGCCAAACTTCAATGCTTTGAGTGTTCCTCCTGCTGGTGCAAAGCAGACCCAACAAAGACCCACAGATATGTCTGCCCTTAATAATCTCTTTGGCCCTCAGAAACCCAAAGTTAGCATGAACCAGTTATCACAACAGAAACCAAATCAGTGGCTTAATCAGTTTGTACCTCCTCAAGGTTCTCCAACTATGGGCAGTTCAGTAATGGGGACACAGATGAACGTGATAGGACAATCTGCTTTTGGTATGCAGGGTAATCCTTTCTTTAACCCACAGAACTTTGCACAGCCACCAACTACTATGACCAATAGCAGTTCAGCTAGCAATGATTTAAAAGATCTTTTTGGGTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMESMLNKLKSTVTKVTADVTSAVMGNPVTREFDVGRHIASGGNGLAWKIFNGTKKSTKQEVAVFVFDKKLIDKYQKFEKDQIIDSLKRGVQQLTRLRHPRLLTVQHPLEESRDCLAFCTEPVFASLANVLGNWENLPSPISPDIKDYKLYDVETKYGLLQVSEGLSFLHSSVKMVHGNITPENIILNKSGAWKIMGFDFCVSSTNPSEQEPKFPCKEWDPNLPSLCLPNPEYLAPEYILSVSCETASDMYSLGTVMYAVFNKGKPIFEVNKQDIYKSFSRQLDQLSRLGSSSLTNIPEEVREHVKLLLNVTPTVRPDADQMTKIPFFDDVGAVTLQYFDTLFQRDNLQKSQFFKGLPKVLPKLPKRVIVQRILPCLTSEFVNPDMVPFVLPNVLLIAEECTKEEYVKLILPELGPVFKQQEPIQILLIFLQKMDLLLTKTPPDEIKNSVLPMVYRALEAPSIQIQELCLNIIPTFANLIDYPSMKNALIPRIKNACLQTSSLAVRVNSLVCLGKILEYLDKWFVLDDILPFLQQIPSKEPAVLMGILGIYKCTFTHKKLGITKEQLAGKVLPHLIPLSIENNLNLNQFNSFISVIKEMLNRLESEHKTKLEQLHIMQEQQKSLDIGNQMNVSEEMKVTNIGNQQIDKVFNNIGADLLTGSESENKEDGLQNKHKRASLTLEEKQKLAKEQEQAQKLKSQQPLKPQVHTPVATVKQTKDLTDTLMDNMSSLTSLSVSTPKSSASSTFTSVPSMGIGMMFSTPTDNTKRNLTNGLNANMGFQTSGFNMPVNTNQNFYSSPSTVGVTKMTLGTPPTLPNFNALSVPPAGAKQTQQRPTDMSALNNLFGPQKPKVSMNQLSQQKPNQWLNQFVPPQGSPTMGSSVMGTQMNVIGQSAFGMQGNPFFNPQNFAQPPTTMTNSSSASNDLKDLFG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU27775
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 3 silent mutations L394 E611 L723
• Low Temp: No