cDNA Clone POLE2 (mouse)

• Plasmid: pGEX6P1 POLE2 Mouse
• Parent Plasmid: pGEX6P1
• Expressed: GST POLE2 mouse
• Genbank: NP_035263.1
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCCCGGAATTCCCGGGTCGACTCGAGATGGCGCCGGAGCGGCTGCGGAGCCGCACGGTCTCAGCTTTCAAGCTGCGGGGCTTGCTGCTCAGGAGTGAAGCCACCAAGTATCTGACTGAAGCTCTCCAGTCTGTCACTGAATTGGAGCTAGAGGAGAACCTAGGAAAGATCATCGATGCAGTTGAAAAGCAGCCCTTGTCCTCGAACATGATCGAACGATCTGTCGTGGAGGCAGCTGTGCAAGAGTGCAGTCAGTCTGTGGATGAGACTATAGAGCACGTTTTCAATATCATTGGAGCTTTTGATATTCCACGTTTTATATATAATTCAGAAAGGAAAAAATTTCTTCCTCTATCCATGACTAATCATGCTGCACCAACCTTATTTGGAACAGCAAGAGACAAAGCCGAGCTGTATCTTGAACGCTATACCATTTTACACCAGAGGACACACAGACATGAATTGTTCACTCCTCCGGTGATAGGCTCACACCTTGAAGAAAGTGGGAGCAAGTTCCAGCTTAAAACTATAGAAACCTTACTGGGTAGCACAAGCAAAGTTGGTGACGTGATCGTCCTGGGGATGATAACCCAGCTGAAAGAGGGGAAGTTTTTCCTGGAAGATCCTACTGGAACAGTACAACTGGACCTTAGTAAAGCTCAGTTCCACAGTGGCTTATACACCGAGGCCTGCTTCGTCCTGGCAGAAGGTTGGTTTGAAGATCAAGTGTTTCACGCCAATGCCTTTGGATTTCCTCCCACTGAGCCCTCCAGCACCACAAGGGCGTACTATGGAAATATTAATTTTTTTGGAGGGCCTTCTAATGCATCAGTGAAGACGTATACAAAGCTGAGACAGCTGGAAGATGAGAACAAGGACGCCATGTTTGTGATTGTGTCTGATGTTTGGTTGGACCAAGTGCAAGTGTTGGAGAAGTTTCATGTAATGTTTTCTGGTTACTCGCCAGCACCTCCAACCTGCTTTATTCTGTGTGGTAATTTTTCATCTGCACCATATGGAAAAAATCAAGTCCAGGCTTTGAAAGATTCCCTAAAATCTTTGGCAGACATAATATGTGAATATCCAAGTATCCACCAAAGTAGCCGTTTTGTGTTTGTGCCTGGCCCAGAGGATCCTGGATTTGGTTCCATTTTACCAAGGCCGCCACTCGCTGAAAGCATCACACAGGAATTCAGACAAAGGGTTCCGTTTTCAGTTTTCACGACTAATCCTTGCAGAATTCAGTATTGTACACAAGAAATAATTATCTTCCGTGAAGACTTAGTGAATAAAATGTGTAGAAACTGTGTCCGTTTTCCTAGCAGCAGTTTGGATATTCCTACTCACTTCGTAAAGACCATCTTATCTCAGGGACATCTGGCTCCTCTCCCTCTTTACGTATGCCCCGTCCACTGGGCATATGATTACACGTTGAGAGTGTACCCGGTGCCCGACCTGCTGGTCATTGCAGACAAGTACGACCCGTTCACCGTGACCAACACCGATTGCCTCTGCATAAACCCTGGCTCCTTTCCAAGAAGTGGATTTGCATTCAAAGTTTTTTACCCTTCCAGTAAAACAGTAGAAGACAGCAAACTTCAGGGCTTTTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFPGRLEMAPERLRSRTVSAFKLRGLLLRSEATKYLTEALQSVTELELEENLGKIIDAVEKQPLSSNMIERSVVEAAVQECSQSVDETIEHVFNIIGAFDIPRFIYNSERKKFLPLSMTNHAAPTLFGTARDKAELYLERYTILHQRTHRHELFTPPVIGSHLEESGSKFQLKTIETLLGSTSKVGDVIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEGWFEDQVFHANAFGFPPTEPSSTTRAYYGNINFFGGPSNASVKTYTKLRQLEDENKDAMFVIVSDVWLDQVQVLEKFHVMFSGYSPAPPTCFILCGNFSSAPYGKNQVQALKDSLKSLADIICEYPSIHQSSRFVFVPGPEDPGFGSILPRPPLAESITQEFRQRVPFSVFTTNPCRIQYCTQEIIIFREDLVNKMCRNCVRFPSSSLDIPTHFVKTILSQGHLAPLPLYVCPVHWAYDYTLRVYPVPDLLVIADKYDPFTVTNTDCLCINPGSFPRSGFAFKVFYPSSKTVEDSKLQGF*

• Vector Type: Bacterial
• DU Number: DU27879
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent mutation I349 1 naturally occuring SNP M78V
• Low Temp: No