cDNA Clone PHF2

• Synonyms: CENP-35; GRC5; JHDM1E; KDM7C
• Plasmid: pcDNA5 FRT/TO 3FLAG PHF2
• Parent Plasmid: pcDNA5 FRT/TO 3FLAG
• Expressed: 3FLAG-PHF2
• Genbank: NM_005392.3 - extra Q residue after K729 (common variant)
• Tag: 3FLAG
• Species: Human

Sequence of Insert

GGATCCATGGCGACGGTGCCCGTGTACTGCGTCTGCCGGCTCCCCTACGACGTTACCCGCTTCATGATCGAGTGCGACGCCTGCAAGGACTGGTTCCACGGCAGCTGTGTTGGGGTGGAAGAGGAGGAGGCGCCCGACATCGACATATACCACTGCCCAAACTGTGAGAAAACCCATGGGAAGTCCACCTTAAAGAAGAAGCGGACCTGGCACAAACACGGCCCGGGGCAAGCGCCTGACGTCAAGCCCGTGCAGAATGGCAGCCAGCTCTTCATCAAGGAGCTGCGGAGCCGGACCTTTCCCAGTGCTGAAGACGTGGTGGCCCGTGTGCCAGGAAGTCAGCTCACGCTGGGCTACATGGAGGAGCACGGCTTCACCGAGCCCATCCTCGTCCCTAAGAAAGACGGGCTGGGTCTGGCTGTCCCGGCCCCCACGTTCTATGTCAGTGACGTCGAGAACTACGTGGGGCCGGAACGGAGTGTGGATGTGACAGATGTCACCAAGCAGAAGGACTGCAAGATGAAGCTGAAGGAGTTTGTGGACTATTACTACAGCACCAACCGCAAGCGGGTCCTCAACGTCACCAACCTCGAGTTCTCTGACACCCGAATGTCCAGCTTCGTGGAGCCACCTGACATTGTAAAGAAACTGTCATGGGTAGAAAACTACTGGCCAGATGATGCATTGCTGGCCAAGCCCAAAGTGACCAAGTACTGCCTAATCTGCGTGAAGGACAGTTACACCGACTTCCACATCGACTCTGGGGGCGCCTCTGCCTGGTACCACGTGCTCAAGGGGGAGAAGACCTTCTATCTCATCAGGCCGGCCTCGGCCAACATCTCCCTGTATGAGCGCTGGCGGTCTGCCTCTAACCACAGCGAGATGTTCTTTGCTGACCAGGTCGACAAATGCTACAAGTGCATCGTCAAGCAGGGCCAGACCCTCTTCATCCCCTCAGGCTGGATCTACGCCACACTCACCCCTGTGGACTGCCTGGCCTTCGCGGGACATTTCCTCCACAGCCTGAGTGTGGAGATGCAGATGAGAGCATATGAAGTGGAAAGGAGGTTGAAACTGGGCAGCCTGACTCAGTTTCCCAACTTTGAAACTGCGTGCTGGTACATGGGGAAGCACCTGCTGGAGGCATTCAAAGGCTCTCACAAATCTGGGAAGCAGCTGCCCCCTCATCTAGTCCAAGGAGCTAAAATTCTCAATGGTGCTTTCCGATCGTGGACGAAGAAGCAGGCTTTGGCAGAGCATGAGGACGAGCTCCCGGAGCACTTCAAACCTTCACAGCTAATCAAAGACCTGGCCAAAGAGATCCGGCTCAGTGAGAATGCCTCCAAAGCCGTCCGACCGGAAGTGAATACTGTCGCCTCGTCAGATGAGGTGTGTGACGGGGACCGGGAGAAGGAGGAGCCCCCGTCTCCCATTGAGGCCACCCCGCCTCAATCCCTCCTGGAGAAAGTGTCCAAAAAAAAGACTCCCAAAACTGTGAAGATGCCCAAGCCATCCAAAATCCCCAAGCCCCCGAAGCCCCCTAAGCCCCCAAGGCCCCCCAAAACGCTGAAGCTCAAAGATGGAGGCAAGAAGAAAGGGAAGAAGTCCCGGGAGTCAGCCTCACCCACCATCCCCAACCTGGACCTGCTCGAAGCCCACACCAAGGAGGCACTGACCAAGATGGAGCCGCCCAAGAAGGGCAAGGCCACAAAGAGTGTCCTGAGTGTGCCCAACAAAGATGTGGTTCACATGCAGAATGATGTGGAGAGGCTGGAAATTCGAGAGCAAACCAAGAGCAAGTCAGAGGCCAAGTGGAAGTACAAGAACAGCAAACCTGACTCCTTACTGAAGATGGAAGAGGAGCAGAAGCTAGAGAAGTCGCCTCTAGCTGGAAACAAAGACAATAAGTTCTCTTTTTCTTTCTCCAACAAGAAACTCCTCGGCTCCAAGGCTCTCAGGCCCCCGACGAGCCCTGGTGTGTTCGGGGCCTTGCAGAACTTCAAGGAGGACAAGCCCAAGCCCGTGCGGGATGAGTATGAGTACGTGTCGGATGACGGTGAGCTCAAGATCGACGAGTTTCCCATCAGGAGGAAGAAAAACGCCCCGAAAAGGGACTTGTCCTTCTTGTTGGATAAGAAGGCTGTGCTGCCCACGCCTGTCACGAAGCCAAAGCTGGACTCGGCAGCGTACAAGCAGAGTGATGACTCCTCGGACGAGGGTTCGCTGCACATCGACACAGACACCAAGCCCGGCCGCAATGCCAGAGTCAAGAAGGAGAGTGGGAGCTCGGCAGCTGGCATCTTGGACCTGCTGCAGGCCAGTGAGGAGGTTGGCGCGCTGGAGTACAACCCCAGCAGCCAGCCCCCGGCCTCCCCCAGCACACAGGAAGCCATTCAGGGAATGCTGTCCATGGCCAACCTGCAGGCCTCCGACTCCTGCCTGCAGACCACGTGGGGAGCTGGCCAGGCCAAGGGGAGCTCGCTGGCTGCCCATGGTGCCCGGAAGAATGGGGGTGGCAGTGGCAAGAGTGCAGGCAAACGACTGCTGAAGAGGGCTGCCAAGAACAGTGTCGACCTGGACGACTACGAGGAAGAGCAGGACCACCTGGATGCCTGCTTCAAGGACTCAGACTACGTTTACCCCTCACTGGAGTCAGATGAAGACAACCCCATCTTTAAGTCCCGGTCGAAGAAAAGGAAAGGCTCAGACGACGCTCCCTACAGCCCAACAGCAAGGGTCGGCCCATCGGTGCCAAGACAGGACAGGCCTGTGCGTGAGGGTACACGGGTGGCTTCCATCGAGACCGGGCTGGCGGCTGCTGCAGCTAAGTTGTCCCAGCAGGAGGAGCAGAAAAGCAAGAAAAAAAAGAGTGCCAAGAGGAAGCTGACTCCTAACACCACCTCCCCTTCCACCTCCACCTCCATCTCTGCCGGCACCACCTCCACCTCCACCACGCCAGCCTCTACCACCCCGGCCTCCACCACCCCGGCCTCCACCAGCACGGCCAGCAGCCAGGCCTCGCAGGAGGGCAGCTCGCCAGAGCCCCCGCCTGAGTCGCATAGCAGCAGCCTGGCGGACCATGAGTACACAGCCGCTGGCACCTTCACCGGGGCCCAGGCTGGCCGCACCTCCCAGCCCATGGCCCCTGGGGTCTTTCTCACACAGAGGCGGCCCTCCGCATCGTCTCCAAACAACAACACCGCTGCCAAAGGAAAACGTACGAAAAAGGGCATGGCGACCGCCAAGCAGAGGCTTGGGAAAATTTTGAAAATTCATCGGAACGGGAAACTACTCCTTTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSDYKDDDDKDYKDDDDKGSMATVPVYCVCRLPYDVTRFMIECDACKDWFHGSCVGVEEEEAPDIDIYHCPNCEKTHGKSTLKKKRTWHKH
GPGQAPDVKPVQNGSQLFIKELRSRTFPSAEDVVARVPGSQLTLGYMEEHGFTEPILVPKKDGLGLAVPAPTFYVSDVENYVGPERSVDVTDVTKQKDCK
MKLKEFVDYYYSTNRKRVLNVTNLEFSDTRMSSFVEPPDIVKKLSWVENYWPDDALLAKPKVTKYCLICVKDSYTDFHIDSGGASAWYHVLKGEKTFYLI
RPASANISLYERWRSASNHSEMFFADQVDKCYKCIVKQGQTLFIPSGWIYATLTPVDCLAFAGHFLHSLSVEMQMRAYEVERRLKLGSLTQFPNFETACW
YMGKHLLEAFKGSHKSGKQLPPHLVQGAKILNGAFRSWTKKQALAEHEDELPEHFKPSQLIKDLAKEIRLSENASKAVRPEVNTVASSDEVCDGDREKEE
PPSPIEATPPQSLLEKVSKKKTPKTVKMPKPSKIPKPPKPPKPPRPPKTLKLKDGGKKKGKKSRESASPTIPNLDLLEAHTKEALTKMEPPKKGKATKSV
LSVPNKDVVHMQNDVERLEIREQTKSKSEAKWKYKNSKPDSLLKMEEEQKLEKSPLAGNKDNKFSFSFSNKKLLGSKALRPPTSPGVFGALQNFKEDKPK
PVRDEYEYVSDDGELKIDEFPIRRKKNAPKRDLSFLLDKKAVLPTPVTKPKLDSAAYKQSDDSSDEGSLHIDTDTKPGRNARVKKESGSSAAGILDLLQA
SEEVGALEYNPSSQPPASPSTQEAIQGMLSMANLQASDSCLQTTWGAGQAKGSSLAAHGARKNGGGSGKSAGKRLLKRAAKNSVDLDDYEEEQDHLDACF
KDSDYVYPSLESDEDNPIFKSRSKKRKGSDDAPYSPTARVGPSVPRQDRPVREGTRVASIETGLAAAAAKLSQQEEQKSKKKKSAKRKLTPNTTSPSTST
SISAGTTSTSTTPASTTPASTTPASTSTASSQASQEGSSPEPPPESHSSSLADHEYTAAGTFTGAQAGRTSQPMAPGVFLTQRRPSASSPNNNTAAKGKR
TKKGMATAKQRLGKILKIHRNGKLLL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU58783
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No