cDNA Clone RCK2

• Plasmid: pRS413-ADH Pr 800-RCK2 (YLR248W)-(+)300
• Parent Plasmid: pRS413-ADH Pr

Sequence of Insert

GGATCCATGCTGCTATCACTGAATCAAAAGAACCATTTTCTAGGAAAAAGTCGCATCTTTCGGCAGTCACATTGTACTCCCTTGCTAATAGCGCACCTACTTCAGACATCTACCGTCGAATAAGTTGGCCTTTCCTGGAGTTCATTCCTTTAGACAGCGCCTAGAAAGTGGCAAATCAATACATTCATCAGCCACATTTCATCAGCTGTAGATCTGGAAAAGACGGGTAGCGGTTCGAGTGTTCCGAAAAAGGAAAAAACGAGCCTTATCCTTGGTCAGAGATGCGAGCAGGTAATTCCTTGAGCTCTCATAGGAGTGAAGCTATCCCAGACTCCTTGGATGTCCCTGTATTTGCGGTCAAGTTTGTTCTTTGACTGGTTCGTCATTTCGAGCTCACTTAAAAACAGGAGTTATTCAGCTACATCTTCAAGCTAATAAAACATCCGTTGCGCAGGCACTCTTTTTAGTAGTTGCCATTTCCTTTATTCTTGAAAGTACGAAGGCTGAAAAAGAGCCACTACGATCCAAAAAATCACTGTTAGTTTTAGTCGTCCACGAGTACGTTGGCATAAAGTCTAGTAATCTTTTTACTTTATAGCAATCAAGAGAAAAAAGGAGGATAAAGGGACATTTATTTGTTATTGTTTTTTGTTCTTTTTAAACTTTTTTACTGGCTAGGTTTAAGGTTATAATTATAAATCCAGTGATTAAGAGGAAACCTCGTCAAGAATCCTAGATAATAGTAATCAGCGACAGTTATTTCAACGAAGTCAAACTCTATCTCTGGGCTTTATCTCTCCTATTGACATCTAACTTTTTACAAACGCTTACTAGAAAAGACGGATCGGCCAAAGAAAAAAAAAGAGTGACATCGAAAAAACTAAAGAAAATAAAATCCCAGATAGTTCATACGTTATTTGAAGTACAAACATTTAACGATTGCAAAAGACGAAAGTATTGTTGAGAGTACTGTTTATTTAGAGAGGATCAAACAAAATCTCTTCGATGCTTAAAATAAAGGCCCTTTTCTCGAAAAAGAAACCGGATCAGGCAGATTTGTCTCAGGAATCTAAAAAACCATTCAAGGGTAAGACCAGGTCAAGCGGTACAAATAACAAAGATGTTTCCCAGATTACTTCTTCCCCTAAGAAAAGCTTTCAGGACAAAAATATAGTTCAGTACCCGAGTGTTGTCGCAGATGACCATCATATGAAGTCTTTAACCGATGAATTAGTAACCACAATAGACTCGGACTCTTCCCCGAGTGATAATATTACCACGGAAAATGTGGAAACAGTTACTTCCGTGCCAGCTATCGATGTCCATGAAAATAGTGAAGGTCAATTAAGTTCCGACCCCTTAATATCTGACGAATCTCTTTCGGAACAAAGCGAGATTATCAGTGATATCCAGGATGACAGTACTGATGATGACAATATGGAAGATGAAATCCCGGAAAAATCCTTCCTCGAACAAAAGGAATTGATAGGTTACAAGCTGATCAATAAAATCGGTGAAGGTGCTTTTTCAAAAGTCTTTAGAGCCATACCTGCTAAAAATAGTTCTCATGAATTTTTAACTAAAAACTATAAAGCTGTTGCCATTAAAGTTATTAAAAAGGCAGATTTATCCTCGATTAATGGTGATCATCGTAAGAAGGACAAAGGGAAGGACAGCACTAAAACTTCTTCCAGGGATCAAGCCTTGAAGGAAGTTGCACTACATAAGACGGTTTCCGCTGGTTGTTCACAAATTGTCGCGTTCATAGACTTCCAAGAGACAGATAGCTATTATTATATTATTCAAGAGTTACTAACCGGTGGGGAAATCTTCGGCGAAATTGTTAGGTTGACCTATTTCAGTGAAGATTTATCAAGGCATGTAATCAAACAATTAGCACTGGCTGTTAAACATATGCATTCACTAGGTGTAGTGCATCGTGATATAAAACCTGAGAATCTTCTTTTTGAACCGATTGAATTCACACCCTCTATAAAACCAAAATTGAGGAAATCGGATGATCCGCAAACAAAGGCAGACGAGGGAATTTTCACACCAGGAGTTGGTGGTGGTGGAATTGGTATAGTAAAACTAGCTGATTTTGGTTTGTCTAAACAAATATTTTCCAAGAACACTAAGACTCCCTGTGGTACAGTCGGTTACACTGCCCCTGAAGTTGTCAAAGATGAGCATTATTCTATGAAAGTGGATATGTGGGGGATTGGTTGCGTTTTGTACACAATGTTATGTGGGTTCCCGCCATTCTATGATGAGAAAATTGACACTTTAACTGAAAAAATATCAAGGGGTGAGTATACCTTTCTGAAACCTTGGTGGGATGAAATCAGCGCCGGTGCCAAGAATGCCGTGTCTAAGCTATTAGAACTAGAGCCGTCTAAAAGATACGACATTGACCAGTTTTTGGACGACCCATGGTTAAATACATTCGATTGTTTACCAAAGGAGGGCGAATCTTCACAAAAGAAAGCAGGTACTTCCGAAAGACGCCATCCGCATAAGAAACAATTCCAACTATTTCAAAGAGACTCCTCGCTACTGTTTTCACCAGCTGCTGTTGCTATGCGTGACGCCTTTGATATTGGTAATGCTGTGAAACGTACCGAAGAAGACCGTATGGGGACACGTGGAGGATTAGGCTCGCTTGCTGAGGACGAAGAATTGGAAGATAGTTACAGTGGCGCCCAAGGCGATGAACAGCTGGAACAAAATATGTTCCAATTAACGCTGGATACGTCCACGATTCTGCAAAGAAGAAAAAAAGTTCAAGAAAATGACGTAGGGCCTACAATTCCAATAAGCGCCACTATCAGGGAATAGCAACTTTCCCTTCTGTTTCAATCTTTTTACCTATTCCTTTTTAAAAGGTATATATACATTAAACTCCTTCTACAAGTATAAATTTTATACATATCTACAGGGCGTATATATACATAACATTTTAAGATAAGCAAGTGAATGTTGATTCCCGTTTCTTAGTCAACACTTCTTTCTATTTTACCCGATCGTTACCCTATTAAAAAAACAACTTACAATCATTGTTCGCCCCTTCCATACTTACTGCCACTCGCAAAAGGGCCCAACCAGGGCAATTACGTATCAAAAAATCATGACAGCTCGAG

Amino Acid Sequence

MLKIKALFSKKKPDQADLSQESKKPFKGKTRSSGTNNKDVSQITSSPKKSFQDKNIVQYPSVVADDHHMKSLTDELVTTIDSDSSPSDNITTENVETVTSVPAIDVHESSEGQLSSDPLISDESLSEQSEIISDIQDDSTDDDNMEDEIPEKSFLEQKELIGYKLINKIGEGAFSKVFRAIPAKNSSNEFLTKNYKAVAIKVIKKADLSSINGDHRKKDKGKDSTKTSSRDQVLKEVALHKTVSAGCSQIVAFIDFQETDSYYYIIQELLTGGEIFGEIVRLTYFSEDLSRHVIKQLALAVKHMHSLGVVHRDIKPENLLFEPIEFTRSIKPKLRKSDDPQTKADEGIFTPGVGGGGIGIVKLADFGLSKQIFSKNTKTPCGTVGYTAPEVVKDEHYSMKVDMWGIGCVLYTMLCGFPPFYDEKIDTLTEKISRGEYTFLKPWWDEISAGAKNAVAKLLELEPSKRYDIDQFLDDPWLNTFDCLPKEGESSQKKAGTSERRHPHKKQFQLFQRDSSLLFSPAAVAMRDAFDIGNAVKRTEEDRMGTRGGLGSLAEDEELEDSYSGAQGDEQLEQNMFQLTLDTSTILQRRKKVQENDVGPTIPISATIRE*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU59741
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cloning of the budding yeast RCK2 (YLR248W) including 1000 bp from its own promoter and 300 bp from its own 3'UTR in the pRS413 centromeric vector.
• Low Temp: No