Plasmid
pET24-Ubiquitin 1-72 del LRGG (silent a220c)
Parent Plasmid
pET24a original
Expressed
untagged Ubiquitin 1-72 del LRGG
Genbank
P62988*
Tag
untagged
Species
Human
Sequence of Insert
View Sequence
CATACATATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTTGATAAGGATCC
Amino Acid Sequence
View Sequence
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVLR*
EDGRTLSDYNIQKESTLHLVLR*
Vector Type
Bacterial
Antibiotic
Kan
DU Number
DU59815
Unit Source
Location
PPU
MTA
Notes
please remove C-terminal LRGG from 20027
.
Bacterial expression construct for untagged ubiquitin (Arg73 codon AGA mutated to more common CGA)
Differs from reference sequence in having silent mutation at Arg73 (a220c substitution conferred by reverse primer 54389).
T7 tag in pET24a removed by subcloning into the Nde1 site, to give untagged protein.
Estimated MW of expressed protein : 8565 Daltons
.
Bacterial expression construct for untagged ubiquitin (Arg73 codon AGA mutated to more common CGA)
Differs from reference sequence in having silent mutation at Arg73 (a220c substitution conferred by reverse primer 54389).
T7 tag in pET24a removed by subcloning into the Nde1 site, to give untagged protein.
Estimated MW of expressed protein : 8565 Daltons
Low Temp
No
Price
£125.00
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