cDNA Clone RPN12/YFR052W

• Plasmid: p415-GPD 800-RPN12/YFR052W-300
• Parent Plasmid: p415-GPD
• Expressed: RPN12/YFR052W

Sequence of Insert

ACTAGTCAGCAACTCTAGGACTCGATCCTTGGACAGATCTTTGAATTGCCTGGACAAGAGAGGTTTCCCCTGACGCGTAGTGATAGAAGCAGCCAAAACCACCATTGTGTATTTCTTTTGATGGAGCTATAGGTATTCTAATATGATCTATATTCGACGCTGAGGCTAATTGCCCTTGTTCTTGAGGTGTCAACAACCAGATTTTCTAATTTTTCGCTGGCTGTTGCTGGAAAAATGTAAACGCGCAGGGCGGGTAATAATAAAAGTTGAACTATGAAGAACAATAGAAACGCCAAGGTTTACTGACAGAGACATGTTTTAATTCAAGTGATGAGGCGGAAACGTGCAAGATCCTAAATGAAGGATAAAAAGAGTTCTTAAAAAGGGAAGTAAGGAATAACAGAGTAGAAAAACCGAAAAGACAACTTAACAAATCGGCAACACTTTTATGGGGCCCCGCTCGCCTGTGTGCAAGTAGTATTCGACCTGGAACACGCATTTACCACGAGAAGACAGCAATAGTCCGTACAACATTAATTAGTTTCGACAATTGCTCGCCTTTATAAGCCATGCTAGTGCCCAATCAAACACTTTACTTGCCCTGAAGTTCCTTTTTTCGCTAGCCTGTAACTTAAATAAGCCATCTAACCTTTTTTTTCTAAAAATTTTCTTTATTACCCTGTCGGCTTATTTTCTATTCTACACATTATTTGCCACCCATTGAAATTGTAGCTTGTATTAATAGGGAAAAGCCGGAAGTATAACCGGTGGAAAGTACTATTGAAGTGAGATAAGAAGCCATCGTAATGCCCTCGTTAGCCGAATTGACCAAGTCGTTAAGCATAGCCTTTGAAAACGGCGATTATGCCGCGTGTGAGAAGCTCTTGCCCCCTATCAAGATCGAACTTATCAAGAATAACCTTTTAATACCTGACTTATCCATTCAAAATGACATCTATTTGAATGATTTGATGATTACTAAAAGGATCCTGGAAGTAGGTGCCCTTGCTAGCATCCAAACTTTCAATTTTGACAGCTTCGAGAATTACTTCAACCAATTGAAGCCTTACTACTTTAGCAACAATCATAAATTATCTGAATCTGACAAGAAATCGAAGCTGATAAGTCTGTATTTGTTGAACTTATTGTCTCAGAATAACACAACCAAGTTTCACTCGGAATTGCAGTATCTAGATAAACATATCAAGAACTTGGAAGACGATTCACTTTTGTCTTACCCTATCAAACTAGACAGATGGCTCATGGAAGGGTCGTACCAGAAAGCATGGGATCTTCTGCAATCTGGGTCGCAGAATATATCAGAATTCGACTCTTTTACCGATATCCTAAAATCAGCTATAAGAGACGAAATTGCTAAAAATACCGAGCTATCCTACGACTTTCTCCCTCTCTCCAACATAAAGGCTTTGCTCTTTTTCAACAACGAAAAAGAAACTGAAAAATTTGCACTAGAGAGAAACTGGCCTATTGTCAACTCGAAAGTTTACTTCAATAACCAATCAAAGGAGAAAGCTGATTACGAAGATGAAATGATGCATGAAGAAGACCAAAAGACAAACATTATCGAAAAAGCAATGGATTATGCCATAAGTATTGAAAATATTGTGTAATATTTGTATATATAATATGTGCACAAAATTACTACAAAATAAACAACTTTTTCTCTCCCGGAGAACAAAGTAAGTGGAAAAAATTATACTAATAACAATCTAAAAATGTTTATTAGCTAGGATTATACACATAAATATATATATATGTGTGTGTCTATATTTACATATACTAGACCGCAAAAAAAACATAAGGGCATCACTCATAAGAATAATAATATTAAGGGAGGGAAAAACACATTTATATTTCATTACATTTTTTTCATTAAGCGCCAATGATACCAAGAGACTTACCTTCGGCAACCCGGG

Amino Acid Sequence

MPSLAELTKSLSIAFENGDYAACEKLLPPIKIELIKNNLLIPDLSIQNDIYLNDLMITKRILEVGALASIQTFNFDSFENYFNQLKPYYFSNNHKLSESDKKSKLISLYLLNLLSQNNTTKFHSELQYLDKHIKNLEDDSLLSYPIKLDRWLMEGSYQKAWDLLQSGSQNISEFDSFTDILKSAIRDEIAKNTELSYDFLPLSNIKALLFFNNEKETEKFALERNWPIVNSKVYFNNQSKEKADYEDEMMHEEDQKTNIIEKAMDYAISIENIV*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU59990
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: RPN12/YFR052W (ID: 850613) (including 800bp of the 5'UTR and 300 bp of the 3'UTR) in p415-GPD (DU59142) vector.
• Low Temp: No