cDNA Clone PKM2

• Synonyms: pyruvate kinase M1/2
• Plasmid: pBabe puro FLAG-TURBO-ID-PKM2
• Parent Plasmid: pBABE puro FLAG
• Expressed: FLAG-TURBO-ID-PKM2
• Genbank: NM_002654.6
• Tag: FLAG
• Species: Human

Sequence of Insert

AGATCTATGAAAGACAATACTGTGCCTCTGAAGCTGATCGCTCTCCTGGC
TAATGGCGAGTTCCATAGTGGCGAACAGCTGGGAGAAACCCTGGGCATGT
CCAGGGCCGCTATCAACAAGCACATTCAGACTCTGCGCGACTGGGGCGTG
GACGTGTTCACCGTGCCCGGAAAGGGCTACTCTCTGCCCGAGCCTATCCC
GCTGCTGAACGCTAAACAGATTCTGGGACAGCTGGACGGCGGGAGCGTGG
CAGTCCTGCCTGTGGTCGACTCCACCAATCAGTACCTGCTGGATCGAATC
GGCGAGCTGAAGAGTGGGGATGCTTGCATTGCAGAATATCAGCAGGCAGG
GAGAGGAAGCAGAGGGAGGAAATGGTTCTCTCCTTTTGGAGCTAACCTGT
ACCTGAGTATGTTTTGGCGCCTGAAGCGGGGACCAGCAGCAATCGGCCTG
GGCCCGGTCATCGGAATTGTCATGGCAGAAGCGCTGCGAAAGCTGGGAGC
AGACAAGGTGCGAGTCAAATGGCCCAATGACCTGTATCTGCAGGATAGAA
AGCTGGCAGGCATCCTGGTGGAGCTGGCCGGAATAACAGGCGATGCTGCA
CAGATCGTCATTGGCGCCGGGATTAACGTGGCTATGAGGCGCGTGGAGGA
AAGCGTGGTCAATCAGGGCTGGATCACACTGCAGGAAGCAGGGATTAACC
TGGACAGGAATACTCTGGCCGCTACGCTGATCCGAGAGCTGCGGGCAGCC
CTGGAACTGTTCGAGCAGGAAGGCCTGGCTCCATATCTGCCACGGTGGGA
GAAGCTGGATAACTTCATCAATAGACCCGTGAAGCTGATCATTGGGGACA
AAGAGATTTTCGGGATTAGCCGGGGGATTGATAAACAGGGAGCCCTGCTG
CTGGAACAGGACGGAGTTATCAAACCCTGGATGGGCGGAGAAATCAGTCT
GCGGTCTGCCGAAAAGGGATCC

Amino Acid Sequence

MDYKDDDDKGSMKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKH
IQTLRDWGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDS
TNQYLLDRIGELKSGDACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRL
KRGPAAIGLGPVIGIVMAEALRKLGADKVRVKWPNDLYLQDRKLAGILVE
LAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQEAGINLDRNTLAA
TLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDKEIFGISR
GIDKQGALLLEQDGVIKPWMGGEISLRSAEKGSMSKPHSEAGTAFIQTQQ
LHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKS
GMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGP
EIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKV
VEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVD
LPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKI
ISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRC
NRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAK
GDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAV
EASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYR
GIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRP
GSGFTNTMRVVPVP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29983
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No