cDNA Clone PKM2

• Synonyms: pyruvate kinase M1/2
• Plasmid: pQCXIP puro GFP-PKM2
• Parent Plasmid: pQCXIP puro GFP
• Expressed: GFP-PKM2
• Genbank: NM_002654.4
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGTCGAAGCCCCATAGTGAAGCCGGGACTGCCTTCATTCAGAC
CCAGCAGCTGCACGCAGCCATGGCTGACACATTCCTGGAGCACATGTGCC
GCCTGGACATTGATTCACCACCCATCACAGCCCGGAACACTGGCATCATC
TGTACCATTGGCCCAGCTTCCCGATCAGTGGAGACGTTGAAGGAGATGAT
TAAGTCTGGAATGAATGTGGCTCGTCTGAACTTCTCTCATGGAACTCATG
AGTACCATGCGGAGACCATCAAGAATGTGCGCACAGCCACGGAAAGCTTT
GCTTCTGACCCCATCCTCTACCGGCCCGTTGCTGTGGCTCTAGACACTAA
AGGACCTGAGATCCGAACTGGGCTCATCAAGGGCAGCGGCACTGCAGAGG
TGGAGCTGAAGAAGGGAGCCACTCTCAAAATCACGCTGGATAACGCCTAC
ATGGAAAAGTGTGACGAGAACATCCTGTGGCTGGACTACAAGAACATCTG
CAAGGTGGTGGAAGTGGGCAGCAAGATCTACGTGGATGATGGGCTTATTT
CTCTCCAGGTGAAGCAGAAAGGTGCCGACTTCCTGGTGACGGAGGTGGAA
AATGGTGGCTCCTTGGGCAGCAAGAAGGGTGTGAACCTTCCTGGGGCTGC
TGTGGACTTGCCTGCTGTGTCGGAGAAGGACATCCAGGATCTGAAGTTTG
GGGTCGAGCAGGATGTTGATATGGTGTTTGCGTCATTCATCCGCAAGGCA
TCTGATGTCCATGAAGTTAGGAAGGTCCTGGGAGAGAAGGGAAAGAACAT
CAAGATTATCAGCAAAATCGAGAATCATGAGGGGGTTCGGAGGTTTGATG
AAATCCTGGAGGCCAGTGATGGGATCATGGTGGCTCGTGGTGATCTAGGC
ATTGAGATTCCTGCAGAGAAGGTCTTCCTTGCTCAGAAGATGATGATTGG
ACGGTGCAACCGAGCTGGGAAGCCTGTCATCTGTGCTACTCAGATGCTGG
AGAGCATGATCAAGAAGCCCCGCCCCACTCGGGCTGAAGGCAGTGATGTG
GCCAATGCAGTCCTGGATGGAGCCGACTGCATCATGCTGTCTGGAGAAAC
AGCCAAAGGGGACTATCCTCTGGAGGCTGTGCGCATGCAGCACCTGATTG
CCCGTGAGGCAGAGGCTGCCATCTACCACTTGCAATTATTTGAGGAACTC
CGCCGCCTGGCGCCCATTACCAGCGACCCCACAGAAGCCACCGCCGTGGG
TGCCGTGGAGGCCTCCTTCAAGTGCTGCAGTGGGGCCATAATCGTCCTCA
CCAAGTCTGGCAGGTCTGCTCACCAGGTGGCCAGATACCGCCCACGTGCC
CCCATCATTGCTGTGACCCGGAATCCCCAGACAGCTCGTCAGGCCCACCT
GTACCGTGGCATCTTCCCTGTGCTGTGCAAGGACCCAGTCCAGGAGGCCT
GGGCTGAGGACGTGGACCTCCGGGTGAACTTTGCCATGAATGTTGGCAAG
GCCCGAGGCTTCTTCAAGAAGGGAGATGTGGTCATTGTGCTGACCGGATG
GCGCCCTGGCTCCGGCTTCACCAACACCATGCGTGTTGTTCCTGTGCCGT
GAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSMSKPHSEAG
TAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVE
TLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVA
VALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWL
DYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGV
NLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLG
EKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLA
QKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCI
MLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPT
EATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQT
ARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVV
IVLTGWRPGSGFTNTMRVVPVP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65175
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 65006
• Low Temp: No