cDNA Clone PKM2

• Synonyms: pyruvate kinase M1/2
• Plasmid: pBabeD puro GFP-PKM2
• Parent Plasmid: pBabeD puro GFP
• Expressed: GFP-PKM2
• Genbank: NM_002654.4
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGTCGAAGCCCCATAGTGAAGCCGGGACTGCCTTCATTCAGAC
CCAGCAGCTGCACGCAGCCATGGCTGACACATTCCTGGAGCACATGTGCC
GCCTGGACATTGATTCACCACCCATCACAGCCCGGAACACTGGCATCATC
TGTACCATTGGCCCAGCTTCCCGATCAGTGGAGACGTTGAAGGAGATGAT
TAAGTCTGGAATGAATGTGGCTCGTCTGAACTTCTCTCATGGAACTCATG
AGTACCATGCGGAGACCATCAAGAATGTGCGCACAGCCACGGAAAGCTTT
GCTTCTGACCCCATCCTCTACCGGCCCGTTGCTGTGGCTCTAGACACTAA
AGGACCTGAGATCCGAACTGGGCTCATCAAGGGCAGCGGCACTGCAGAGG
TGGAGCTGAAGAAGGGAGCCACTCTCAAAATCACGCTGGATAACGCCTAC
ATGGAAAAGTGTGACGAGAACATCCTGTGGCTGGACTACAAGAACATCTG
CAAGGTGGTGGAAGTGGGCAGCAAGATCTACGTGGATGATGGGCTTATTT
CTCTCCAGGTGAAGCAGAAAGGTGCCGACTTCCTGGTGACGGAGGTGGAA
AATGGTGGCTCCTTGGGCAGCAAGAAGGGTGTGAACCTTCCTGGGGCTGC
TGTGGACTTGCCTGCTGTGTCGGAGAAGGACATCCAGGATCTGAAGTTTG
GGGTCGAGCAGGATGTTGATATGGTGTTTGCGTCATTCATCCGCAAGGCA
TCTGATGTCCATGAAGTTAGGAAGGTCCTGGGAGAGAAGGGAAAGAACAT
CAAGATTATCAGCAAAATCGAGAATCATGAGGGGGTTCGGAGGTTTGATG
AAATCCTGGAGGCCAGTGATGGGATCATGGTGGCTCGTGGTGATCTAGGC
ATTGAGATTCCTGCAGAGAAGGTCTTCCTTGCTCAGAAGATGATGATTGG
ACGGTGCAACCGAGCTGGGAAGCCTGTCATCTGTGCTACTCAGATGCTGG
AGAGCATGATCAAGAAGCCCCGCCCCACTCGGGCTGAAGGCAGTGATGTG
GCCAATGCAGTCCTGGATGGAGCCGACTGCATCATGCTGTCTGGAGAAAC
AGCCAAAGGGGACTATCCTCTGGAGGCTGTGCGCATGCAGCACCTGATTG
CCCGTGAGGCAGAGGCTGCCATCTACCACTTGCAATTATTTGAGGAACTC
CGCCGCCTGGCGCCCATTACCAGCGACCCCACAGAAGCCACCGCCGTGGG
TGCCGTGGAGGCCTCCTTCAAGTGCTGCAGTGGGGCCATAATCGTCCTCA
CCAAGTCTGGCAGGTCTGCTCACCAGGTGGCCAGATACCGCCCACGTGCC
CCCATCATTGCTGTGACCCGGAATCCCCAGACAGCTCGTCAGGCCCACCT
GTACCGTGGCATCTTCCCTGTGCTGTGCAAGGACCCAGTCCAGGAGGCCT
GGGCTGAGGACGTGGACCTCCGGGTGAACTTTGCCATGAATGTTGGCAAG
GCCCGAGGCTTCTTCAAGAAGGGAGATGTGGTCATTGTGCTGACCGGATG
GCGCCCTGGCTCCGGCTTCACCAACACCATGCGTGTTGTTCCTGTGCCGT
GAGCGGCCGC

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMSKPH
SEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPAS
RSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILY
RPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDEN
ILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGS
KKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVR
KVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEK
VFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDG
ADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPIT
SDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTR
NPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKK
GDVVIVLTGWRPGSGFTNTMRVVPVP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65176
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 33932
• Low Temp: No