cDNA Clone PTPN23

• Synonyms: HD-PTP; HDPTP; PTP-TD14
• Plasmid: pCMV5D HA PTPN23
• Parent Plasmid: pCMV5D HA
• Expressed: HA-PTPN23
• Genbank: NM_015466.4 with three silent changes
• Tag: HA
• Species: Human

Sequence of Insert

gaattcATGGAGGCCGTGCCCCGCATGCCCATGATCTGGCTGGACCTGAA GGAGGCCGGTGACTTTCACTTCCAGCCAGCTGTGAAGAAGTTTGTCCTGA AGAATTATGGAGAGAACCCAGAAGCCTACAATGAAGAACTGAAGAAGCTG GAGTTGCTCAGACAGAATGCTGTCCGTGTCCCACGAGACTTTGAGGGCTG TAGTGTCCTCCGCAAGTACCTCGGCCAGCTTCATTACCTGCAGAGTCGGG TCCCCATGGGCTCGGGCCAGGAGGCCGCTGTCCCTGTCACCTGGACAGAG ATCTTCTCAGGCAAGTCTGTGGCCCATGAGGACATCAAGTACGAGCAGGC CTGTATTCTCTACAACCTTGGAGCGCTGCACTCCATGCTGGGGGCCATGG ACAAGCGGGTGTCTGAGGAGGGCATGAAGGTCTCCTGTACCCATTTCCAG TGCGCAGCCGGCGCCTTCGCCTACCTACGGGAGCACTTCCCTCAAGCCTA CAGCGTCGACATGAGCCGCCAGATCCTTACGCTCAACGTCAACCTCATGC TGGGCCAGGCTCAGGAGTGCCTCCTGGAGAAGTCGATGTTGGACAACAGG AAGAGCTTTCTGGTGGCCCGCATCAGTGCACAGGTGGTAGATTACTACAA GGAGGCATGCCGGGCCTTGGAGAACCCCGACACTGCCTCACTGCTGGGCC GGATCCAGAAGGACTGGAAGAAACTTGTGCAGATGAAGATCTACTACTTC GCAGCCGTGGCTCATCTGCACATGGGAAAGCAGGCCGAGGAGCAGCAGAA GTTCGGGGAGCGGGTTGCATACTTCCAGAGCGCCCTGGACAAGCTCAATG AAGCCATCAAGTTGGCCAAGGGCCAGCCTGACACTGTGCAAGACGCGCTT CGCTTCACTATGGATGTCATTGGGGGAAAGTACAATTCTGCCAAGAAGGA CAACGACTTCATTTACCATGAGGCTGTCCCAGCATTGGACACCCTTCAGC CTGTAAAAGGAGCCCCCTTGGTGAAGCCCTTGCCAGTGAACCCCACAGAC CCAGCTGTTACAGGCCCTGACATCTTTGCCAAACTGGTACCCATGGCTGC CCACGAGGCCTCGTCACTGTACAGTGAGGAGAAGGCCAAGCTGCTCCGGG AGATGATGGCCAAGATTGAGGACAAGAATGAGGTCCTGGACCAGTTCATG GATTCAATGCAGTTGGATCCCGAGACGGTGGACAACCTTGATGCCTACAG CCACATCCCACCCCAGCTCATGGAGAAGTGCGCGGCTCTCAGCGTCCGGC CCGACACTGTCAGGAACCTTGTACAGTCCATGCAAGTGCTGTCAGGTGTG TTCACGGATGTGGAGGCTTCCCTGAAGGACATCAGAGATCTGTTGGAGGA GGATGAGCTGCTAGAGCAGAAGTTTCAGGAGGCGGTGGGCCAGGCAGGGG CCATCTCCATCACCTCCAAGGCTGAGCTGGCAGAGGTGAGGCGAGAATGG GCCAAGTACATGGAAGTCCATGAGAAGGCCTCCTTCACCAACAGTGAGCT GCACCGTGCCATGAACCTGCACGTCGGCAACCTGCGCCTGCTCAGCGGGC CGCTTGACCAGGTCCGGGCTGCCCTGCCCACACCGGCCCTCTCCCCAGAG GACAAGGCCGTGCTGCAAAACCTAAAGCGCATCCTGGCTAAGGTGCAGGA GATGCGGGACCAGCGCGTGTCCCTGGAGCAGCAGCTGCGTGAGCTTATCC AGAAAGATGACATCACTGCCTCGCTGGTCACCACAGACCACTCAGAGATG AAGAAGTTGTTCGAGGAGCAGCTGAAAAAGTATGACCAGCTGAAGGTGTA CCTGGAGCAGAACCTGGCCGCCCAGGACCGTGTCCTCTGTGCACTGACAG AGGCCAACGTGCAGTACGCAGCCGTGCGGCGGGTACTCAGCGACTTGGAC CAAAAGTGGAACTCCACGCTGCAGACCCTGGTGGCCTCGTATGAAGCCTA TGAGGACCTGATGAAGAAGTCGCAGGAGGGCAGGGACTTCTACGCAGATC TGGAGAGCAAGGTGGCTGCTCTGCTGGAGCGCACGCAGTCCACCTGCCAG GCCCGCGAGGCTGCCCGCCAGCAGCTCCTGGACAGGGAGCTGAAGAAGAA GCCGCCGCCACGGCCCACAGCCCCAAAGCCGCTGCTGCCCCGCAGGGAGG AGAGTGAGGCAGTGGAAGCAGGAGACCCCCCTGAGGAGCTGCGCAGCCTC CCCCCTGACATGGTGGCTGGCCCACGACTGCCTGACACCTTCCTGGGAAG TGCCACCCCGCTCCACTTTCCTCCCAGCCCCTTCCCCAGCTCCACAGGCC CAGGACCCCACTATCTCTCAGGCCCCTTGCCCCCTGGTACCTACTCGGGC CCCACCCAGCTGATACAGCCCAGGGCCCCAGGGCCCCATGCAATGCCCGT AGCACCTGGGCCTGCCCTCTACCCAGCCCCTGCCTACACACCGGAGCTGG GCCTTGTGCCCCGATCCTCCCCACAGCATGGCGTGGTGAGCAGTCCCTAT GTGGGGGTAGGGCCGGCCCCACCAGTTGCAGGTCTCCCCTCGGCCCCACC TCCTCAATTCTCAGGCCCCGAGTTGGCCATGGCGGTTCGGCCAGCCACCA CCACAGTAGATAGCATCCAGGCGCCCATCCCCAGCCACACAGCCCCACGG CCAAACCCCACCCCTGCTCCTCCCCCGCCCTGCTTCCCTGTGCCCCCACC GCAGCCACTGCCCACGCCTTACACCTACCCTGCAGGGGCTAAGCAACCCA TCCCAGCACAGCACCACTTCTCTTCTGGGATCCCCGCAGGTTTTCCAGCC CCAAGGATTGGGCCCCAGCCCCAGCCCCATCCTCAGCCCCATCCTTCACA AGCGTTTGGGCCTCAGCCCCCACAGCAGCCCCTTCCACTCCAGCATCCAC ATCTCTTCCCACCCCAGGCCCCAGGACTCCTACCCCCACAATCCCCCTAC CCCTATGCCCCTCAGCCTGGGGTCCTGGGGCAGCCGCCACCCCCCCTACA CACCCAGCTCTACCCAGGTCCCGCTCAAGACCCTCTGCCAGCCCACTCAG GGGCTCTGCCTTTCCCCAGCCCTGGGCCCCCTCAGCCTCCCCATCCCCCA CTGGCATATGGTCCTGCCCCTTCTACCAGACCCATGGGCCCCCAGGCAGC CCCTCTTACCATTCGAGGGCCCTCGTCTGCTGGCCAGTCCACCCCTAGTC CCCACCTGGTGCCTTCACCTGCCCCATCTCCAGGGCCTGGTCCGGTACCC CCTCGCCCCCCAGCAGCAGAACCACCCCCTTGCCTGCGCCGAGGCGCCGC AGCTGCAGACCTGCTCTCCTCCAGCCCGGAGAGCCAGCATGGCGGCACTC AGTCTCCTGGGGGTGGGCAGCCCCTGCTGCAGCCCACCAAGGTGGATGCA GCTGAGGGTCGTCGGCCGCAGGCCCTGCGGCTGATTGAGCGGGACCCCTA TGAACATCCTGAGAGGCTGCGGCAGTTGCAGCAGGAGCTGGAGGCCTTTC GGGGTCAGCTGGGGGATGTGGGAGCTCTGGACACTGTCTGGCGAGAGCTG CAAGATGCGCAGGAACATGATGCCCGAGGCCGTTCCATCGCCATTGCCCG CTGCTACTCACTGAAGAACCGGCACCAGGATGTCATGCCCTATGACAGTA ACCGTGTGGTGCTGCGCTCAGGCAAGGATGACTACATCAATGCCAGCTGC GTGGAGGGGCTCTCCCCATACTGCCCCCCGCTAGTGGCAACCCAGGCCCC ACTGCCTGGCACAGCTGCTGACTTCTGGCTCATGGTCCATGAGCAGAAAG TGTCAGTCATTGTCATGCTGGTTTCTGAGGCTGAGATGGAGAAGCAAAAA GTGGCACGCTACTTCCCCACCGAGAGGGGCCAGCCCATGGTGCACGGTGC CCTGAGCCTGGCATTGAGCAGCGTCCGCAGCACCGAAACCCATGTGGAGC GCGTGCTGAGCCTGCAGTTCCGAGACCAGAGCCTCAAGCGCTCTCTTGTG CACCTGCACTTCCCCACTTGGCCTGAGTTAGGCCTGCCCGACAGCCCCAG CAACTTGCTGCGCTTCATCCAGGAGGTGCACGCACATTACCTGCATCAGC GGCCGCTGCACACGCCCATCATTGTGCACTGCAGCTCTGGTGTGGGCCGC ACGGGAGCCTTTGCACTGCTCTATGCAGCTGTGCAGGAGGTGGAGGCTGG GAACGGAATCCCTGAGCTGCCTCAGCTGGTGCGGCGCATGCGGCAGCAGA GAAAGCACATGCTGCAGGAGAAGCTGCACCTCAGGTTCTGCTATGAGGCA GTGGTGAGACACGTGGAGCAGGTCCTGCAGCGCCATGGTGTGCCTCCTCC ATGCAAACCCTTGGCCAGTGCAAGCATCAGCCAGAAGAACCACCTTCCTC AGGACTCCCAGGACCTGGTCCTCGGTGGGGATGTGCCCATCAGCTCCATC CAGGCCACCATTGCCAAGCTCAGCATTCGGCCTCCTGGGGGGTTGGAGTC CCCGGTTGCCAGCTTGCCAGGCCCTGCAGAGCCCCCAGGCCTCCCGCCAG CCAGCCTCCCAGAGTCTACCCCAATCCCATCTTCCTCCCCACCCCCCCTT TCCTCCCCACTACCTGAGGCTCCCCAGCCTAAGGAGGAGCCGCCAGTGCC TGAAGCCCCCAGCTCGGGGCCCCCCTCCTCCTCCCTGGAATTGCTGGCCT CCTTGACCCCAGAGGCCTTCTCCCTGGACAGCTCCCTGCGGGGCAAACAG CGGATGAGCAAGCATAACTTTCTGCAGGCCCATAACGGGCAAGGGCTGCG GGCCACCCGGCCCTCTGACGACCCCCTCAGCCTTCTGGATCCACTCTGGA CACTCAACAAGACCTGActcgag

Amino Acid Sequence

MYPYDVPDYAGSFEEFMEAVPRMPMIWLDLKEAGDFHFQPAVKKFVLKNYGENPEAYNEELKKLELLRQNAVRVPRDFEGCSVLRKYLGQLHYLQSRVPM
GSGQEAAVPVTWTEIFSGKSVAHEDIKYEQACILYNLGALHSMLGAMDKRVSEEGMKVSCTHFQCAAGAFAYLREHFPQAYSVDMSRQILTLNVNLMLGQ
AQECLLEKSMLDNRKSFLVARISAQVVDYYKEACRALENPDTASLLGRIQKDWKKLVQMKIYYFAAVAHLHMGKQAEEQQKFGERVAYFQSALDKLNEAI
KLAKGQPDTVQDALRFTMDVIGGKYNSAKKDNDFIYHEAVPALDTLQPVKGAPLVKPLPVNPTDPAVTGPDIFAKLVPMAAHEASSLYSEEKAKLLREMM
AKIEDKNEVLDQFMDSMQLDPETVDNLDAYSHIPPQLMEKCAALSVRPDTVRNLVQSMQVLSGVFTDVEASLKDIRDLLEEDELLEQKFQEAVGQAGAIS
ITSKAELAEVRREWAKYMEVHEKASFTNSELHRAMNLHVGNLRLLSGPLDQVRAALPTPALSPEDKAVLQNLKRILAKVQEMRDQRVSLEQQLRELIQKD
DITASLVTTDHSEMKKLFEEQLKKYDQLKVYLEQNLAAQDRVLCALTEANVQYAAVRRVLSDLDQKWNSTLQTLVASYEAYEDLMKKSQEGRDFYADLES
KVAALLERTQSTCQAREAARQQLLDRELKKKPPPRPTAPKPLLPRREESEAVEAGDPPEELRSLPPDMVAGPRLPDTFLGSATPLHFPPSPFPSSTGPGP
HYLSGPLPPGTYSGPTQLIQPRAPGPHAMPVAPGPALYPAPAYTPELGLVPRSSPQHGVVSSPYVGVGPAPPVAGLPSAPPPQFSGPELAMAVRPATTTV
DSIQAPIPSHTAPRPNPTPAPPPPCFPVPPPQPLPTPYTYPAGAKQPIPAQHHFSSGIPAGFPAPRIGPQPQPHPQPHPSQAFGPQPPQQPLPLQHPHLF
PPQAPGLLPPQSPYPYAPQPGVLGQPPPPLHTQLYPGPAQDPLPAHSGALPFPSPGPPQPPHPPLAYGPAPSTRPMGPQAAPLTIRGPSSAGQSTPSPHL
VPSPAPSPGPGPVPPRPPAAEPPPCLRRGAAAADLLSSSPESQHGGTQSPGGGQPLLQPTKVDAAEGRRPQALRLIERDPYEHPERLRQLQQELEAFRGQ
LGDVGALDTVWRELQDAQEHDARGRSIAIARCYSLKNRHQDVMPYDSNRVVLRSGKDDYINASCVEGLSPYCPPLVATQAPLPGTAADFWLMVHEQKVSV
IVMLVSEAEMEKQKVARYFPTERGQPMVHGALSLALSSVRSTETHVERVLSLQFRDQSLKRSLVHLHFPTWPELGLPDSPSNLLRFIQEVHAHYLHQRPL
HTPIIVHCSSGVGRTGAFALLYAAVQEVEAGNGIPELPQLVRRMRQQRKHMLQEKLHLRFCYEAVVRHVEQVLQRHGVPPPCKPLASASISQKNHLPQDS
QDLVLGGDVPISSIQATIAKLSIRPPGGLESPVASLPGPAEPPGLPPASLPESTPIPSSSPPPLSSPLPEAPQPKEEPPVPEAPSSGPPSSSLELLASLT
PEAFSLDSSLRGKQRMSKHNFLQAHNGQGLRATRPSDDPLSLLDPLWTLNKT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU67217
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Compared to NCBI ref clone has silent changes at Thr329 (ACT>ACC), Pro933 (CCG>CCA), Glu1166 (GAG>GAA), Pr01545 (CCG>CCA)
• Low Temp: No