cDNA Clone RUD3

• Plasmid: pRS413 ADH Pr 400-RUD3/YOR216C-200 S55A
• Parent Plasmid: pRS413 ADH Pr
• Expressed: RUD3/YOR216C S55A
• Genbank: 854391

Sequence of Insert

GGATCCCCACCTGTCTCGTAGGTCTGGATGTTGACGATCTTACGGATTTCTTATTTTTACCAAAGAAATCAGAAATATTGACCATTTTCTTCAGCTTAGTTTATTATGAAGTGACTTGAAGTTATAAAATTAACTGCGAGATACCCAATTCCTTATTGTGTAAGGATCAAGCAGCGTATTCAACGAAGCCACTGTTTTCGCTGAGAGATCGGCTGTTCCTTCTCATGTTGATTTTTTTTTCAGTACTTCTTTTTGAATTTCGTGCTCATTTCGATACGAAAAATGGAAACAAACAGAAAGACCATTCATGAAGAACAAGAAAATCTTGTATAAACAGCTATATAGTAGATGCTTGTGTATACTTTCCACTTGATCCCCTCAAACAATTGCATTTTTTTTAATTAGAATGGGTAAAAATAAGAAGAAAACAGGTAAGAAGGCCAAGAGTCATCCTCATGTGGAGGACGTTGATGAAACCGTCAATAAACCTGAAGAAATCATCAATTCTGTAAACGTCACCGTTCCTCCAAAAATGTCCACAGATCCAGAGGCTGATGGTATCGTGGCAGCCCCGGACGATGAGGGGAAAGATCTCTCTGAAGGAGTAGACAAACAAAAAGTAAATGACGGCCTGACTGTAGACACCATAAATCCTTTAGAGGACAAAAAGGCTGGTGATGAGATGAAGGAACTAAGGGAAGAAATTGAACGTTTAAAATTGGAACTAAGCCACAAGAAGGATCAAGAAACGCCTAATGAAGACTTCAAAAATGAACTAGCAAATGTTATTAAGGAAAGAGACGAATTCAAAACACAGTATGACACATTATTGAGCAAGATATCTTCTATGAAATCAATTTTTAATAAGATGAAAGAAGCACAAAAACAATTAGAGGAAGTGCAGGAGCAGCTTACTGAATATGAATCACAAAATCTGAAATTGAAGAAGAAACTTGAAGCTACCAAGACCGAAAACTCTGAATTACAATCCACGATTGTGACCCTAAATACAGAGTTAGAAAACCTAGAAAAGGAGCAAGAAAGTACAGAAGAGGTTTTTTTGGAATACGAATCAAGGATAGAAGCTTTGGAAGATGAAAAACACGACATAATTGAAAAACATAGTAAGGAACTTAACACTTACAGGAAGGAAAAAGATCAATTAAATCTCCAAGTTCAGGAACTCATGATAATCTTAGAAAATAATAAACAAGATATATCTGACTTGAGAACAGAAAGAGATGAATTGAGACAGGCTTTGGAATCCCATGAAAAGGAGAAAGCAGTATTAAAGAATTCTCTAAATGATCTGGAATTGAAGATAGAAGAAGTTGACAACAAAAGGGAAGAAGAGGCTAGAGAGAGAGACCAGGAAGTCAAATCTTTACGATCCCAACTTGACACGGAGATAGAAACGCACAACAATGACACAGAAGCTTTAGAAAGCATGAAAAAACAGCTAGAAGCCATGAAGGAGGACGCCTCTATGAAGGAAAAATATGAGGAAGAATCTAAGCAGCACATCTTACAGATCGGAAAACTACGTCACGAAGCCATTATACTAAATGAGCATTTAACAAAGGCACTGGCCATGTTGAAGAAATCAAGCGATTCTGAATCCGTTGATAAAGAACTGATATCCAATCTATTGATATCTTTCGTGTCCATACCCAGAGCAGACCCAAGAAAGTTTGAAGTTCTTGAATTACTGTCCAATTTCTTAAACTGGGATGAAGATAAGAAACAACAGGCCGGCTTGATCTCGAATAATGAGTCTAAGAATTCAAGCGCTGTATCGAGAACGGAAAGCTTTGTATCGCTTTGGACCAACTACCTCGAGAAAGAGAGTGAGAAAGACTAATCGGGCAAACTAAGTCTAGTTCAGAAGAGCATACGTACTTAGTGTAGCATCACCCAATTTATACTCTATAAACGATAAATGATATAAATCACATCTACCCGGCATCTGGGACTTTTCGGGGGGAAGGACTCAAAATTAAAGGGAAGAAAAAGGATCTGCAACATTTTTTACAGAAGTAATGAACTGGAGAAAAAAGACTTGTCGAC

Amino Acid Sequence

MGKNKKKTGKKAKSHPHVEDVDETVNKPEEIINSVNVTVPPKMSTDPEADGIVAAPDDEGKDLSEGVDKQKVNDGLTVDTINPLEDKKAGDEMKELREEIERLKLELSHKKDQETPNEDFKNELANVIKERDEFKTQYDTLLSKISSMKSIFNKMKEAQKQLEEVQEQLTEYESQNLKLKKKLEATKTENSELQSTIVTLNTELENLEKEQESTEEVFLEYESRIEALEDEKHDIIEKHSKELNTYRKEKDQLNLQVQELMIILENNKQDISDLRTERDELRQALESHEKEKAVLKNSLNDLELKIEEVDNKREEEARERDQEVKSLRSQLDTEIETHNNDTEALESMKKQLEAMKEDASMKEKYEEESKQHILQIGKLRHEAIILNEHLTKALAMLKKSSDSESVDKELISNLLISFVSIPRADPRKFEVLELLSNFLNWDEDKKQQAGLISNNESKNSSAVSRTESFVSLWTNYLEKESEKD*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU66151
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mutation of RUD3 - S55A from pRS413 ADH Pr 400-RUD3/YOR216C-200 (DU59802)
• Low Temp: No