cDNA Clone PPM1J

• Synonyms: PP2CZ; PP2Czeta; PPP2CZ
• Plasmid: pcDNA FRT TO GFP PPM1J D279A
• Parent Plasmid: pcDNA FRT TO GFP
• Expressed: GFP-PPM1J D279A
• Genbank: NM_005167.5
• Tag: GFP

Sequence of Insert

GGATCCATGCTAAACCGGGTGCGCTCGGCCGTGGCGCACCTGGTGAGCTCCGGGGGCGCTCCGCCTCCGCGCCCCAAATCCCCGGACCTGCCCAACGCCGCCTCGGCGCCGCCCGCCGCCGCTCCAGAAGCGCCCAGGAGCCCTCCCGCGAAGGCTGGGAGCGGGAGCGCGACGCCCGCGAAGGCTGTTGAGGCTCGAGCGAGCTTCTCCAGACCGACCTTTCTGCAGCTGAGCCCCGGGGGGCTGCGACGCGCCGATGACCACGCGGGCCGGGCTGTGCAAAGCCCCCCGGACACGGGCCGCCGCCTGCCCTGGAGCACAGGCTACGCCGAGGTCATCAATGCTGGCAAGAGTCGGCACAATGAGGACCAGGCTTGCTGTGAAGTGGTGTATGTGGAAGGTCGGAGGAGTGTTACAGGAGTACCTAGGGAGCCTAGCCGAGGCCAGGGACTCTGCTTCTACTACTGGGGCCTATTTGATGGGCATGCAGGGGGCGGAGCTGCTGAAATGGCCTCACGGCTCCTGCATCGCCATATCCGAGAGCAGCTAAAGGACCTGGTAGAGATACTTCAGGACCCTTCGCCACCACCCCTCTGCCTCCCAACCACTCCGGGGACCCCAGATTCCTCCGATCCCTCTCACTTGCTTGGCCCTCAGTCCTGCTGGTCTTCACAGAAGGAAGTGAGCCACGAGAGCCTGGTAGTGGGGGCCATTGAGAATGCCTTCCAGCTCATGGATGAGCAGATGGCCCGGGAGCGGCGTGGCCACCAAGTGGAGGGGGGCTGCTGTGCACTGGTTGTGATCTACCTGCTAGGCAAGGTGTACGTGGCCAATGCAGGCGCTAGCAGGGCCATCATTGTCCGGAATGGTGAAATCATTCCAATGTCCCGGGAGTTTACCCCGGAGACTGAGCGCCAGCGTCTTCAGCTGCTTGGCTTCCTGAAACCAGAGCTGCTAGGCAGTGAATTCACCCACCTTGAGTTCCCCCGCAGAGTTCTGCCCAAGGAGCTGGGGCAGAGGATGTTGTACCGGGACCAGAACATGACCGGCTGGGCCTACAAAAAGATCGAGCTGGAGGATCTCAGGTTTCCTCTGGTCTGTGGGGAGGGCAAAAAGGCTCGGGTGATGGCCACCATTGGGGTGACCCGAGGCTTGGGAGACCACAGCCTTAAGGTCTGCAGTTCCACCCTGCCCATCAAGCCCTTTCTCTCCTGCTTCCCTGAGGTACGAGTGTATGACCTGACACAATATGAGCACTGCCCAGATGATGTGCTAGTCCTGGGAACAGATGGCCTGTGGGATGTCACTACTGACTGTGAGGTAGCTGCCACTGTGGACAGGGTGCTGTCGGCCTATGAGCCTAATGACCACAGCAGGTATACAGCTCTGGCCCAAGCTCTGGTCCTGGGGGCCCGGGGTACCCCCCGAGACCGTGGCTGGCGTCTCCCCAACAACAAGCTGGGTTCCGGGGATGACATCTCTGTCTTCGTCATCCCCCTGGGAGGGCCAGGCAGTTACTCCTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMLNRVRSAVAHLVSSGGAPPPRPKSPDLPNAASAPPAAAPEAPRSPPAKAGSGSATPAKAVEARASFSRPTFLQLSPGGLRRADDHAGRAVQSPPDTGRRLPWSTGYAEVINAGKSRHNEDQACCEVVYVEGRRSVTGVPREPSRGQGLCFYYWGLFDGHAGGGAAEMASRLLHRHIREQLKDLVEILQDPSPPPLCLPTTPGTPDSSDPSHLLGPQSCWSSQKEVSHESLVVGAIENAFQLMDEQMARERRGHQVEGGCCALVVIYLLGKVYVANAGASRAIIVRNGEIIPMSREFTPETERQRLQLLGFLKPELLGSEFTHLEFPRRVLPKELGQRMLYRDQNMTGWAYKKIELEDLRFPLVCGEGKKARVMATIGVTRGLGDHSLKVCSSTLPIKPFLSCFPEVRVYDLTQYEHCPDDVLVLGTDGLWDVTTDCEVAATVDRVLSAYEPNDHSRYTALAQALVLGARGTPRDRGWRLPNNKLGSGDDISVFVIPLGGPGSYS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU66245
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: PPM1J D279A with N-terminal GFP in pcDNA5-FRT/TO
• Low Temp: No