SARS-CoV-2 Nsp13

• Plasmid: pGEX6P1 SARS-CoV-2 Nsp12 P919-Q924 Nsp13 6His
• Parent Plasmid: pGEX6P1
• Expressed: GST 3C SARS-CoV-2 Nsp12 P919-Q924 Nsp13 6His
• Genbank: MN908947.3
• Tag: GST
• Species: SARS-CoV-2

Sequence of Insert

GGATCCCCGCATACCGTGCTGCAGGCGGTGGGCGCGTGCGTGCTGTGCAACAGCCAGACCAGCCTGCGCTGCGGCGCGTGCATTCGCCGCCCGTTTCTGT
GCTGCAAATGCTGCTATGATCATGTGATTAGCACCAGCCATAAACTGGTGCTGAGCGTGAACCCGTATGTGTGCAACGCGCCGGGCTGCGATGTGACCGA
TGTGACCCAGCTGTATCTGGGCGGCATGAGCTATTATTGCAAAAGCCATAAACCGCCGATTAGCTTTCCGCTGTGCGCGAACGGCCAGGTGTTTGGCCTG
TATAAAAACACCTGCGTGGGCAGCGATAACGTGACCGATTTTAACGCGATTGCGACCTGCGATTGGACCAACGCGGGCGATTATATTCTGGCGAACACCT
GCACCGAACGCCTGAAACTGTTTGCGGCGGAAACCCTGAAAGCGACCGAAGAAACCTTTAAACTGAGCTATGGCATTGCGACCGTGCGCGAAGTGCTGAG
CGATCGCGAACTGCATCTGAGCTGGGAAGTGGGCAAACCGCGCCCGCCGCTGAACCGCAACTATGTGTTTACCGGCTATCGCGTGACCAAAAACAGCAAA
GTGCAGATTGGCGAATATACCTTTGAAAAAGGCGATTATGGCGATGCGGTGGTGTATCGCGGCACCACCACCTATAAACTGAACGTGGGCGATTATTTTG
TGCTGACCAGCCATACCGTGATGCCGCTGAGCGCGCCGACCCTGGTGCCGCAGGAACATTATGTGCGCATTACCGGCCTGTATCCGACCCTGAACATTAG
CGATGAATTTAGCAGCAACGTGGCGAACTATCAGAAAGTGGGCATGCAGAAATATAGCACCCTGCAGGGCCCGCCGGGCACCGGCAAAAGCCATTTTGCG
ATTGGCCTGGCGCTGTATTATCCGAGCGCGCGCATTGTGTATACCGCGTGCAGCCATGCGGCGGTGGATGCGCTGTGCGAAAAAGCGCTGAAATATCTGC
CGATTGATAAATGCAGCCGCATTATTCCGGCGCGCGCGCGCGTGGAATGCTTTGATAAATTTAAAGTGAACAGCACCCTGGAACAGTATGTGTTTTGCAC
CGTGAACGCGCTGCCGGAAACCACCGCGGATATTGTGGTGTTTGATGAAATTAGCATGGCGACCAACTATGATCTGAGCGTGGTGAACGCGCGCCTGCGC
GCGAAACATTATGTGTATATTGGCGATCCGGCGCAGCTGCCGGCGCCGCGCACCCTGCTGACCAAAGGCACCCTGGAACCGGAATATTTTAACAGCGTGT
GCCGCCTGATGAAAACCATTGGCCCGGATATGTTTCTGGGCACCTGCCGCCGCTGCCCGGCGGAAATTGTGGATACCGTGAGCGCGCTGGTGTATGATAA
CAAACTGAAAGCGCATAAAGATAAAAGCGCGCAGTGCTTTAAAATGTTTTATAAAGGCGTGATTACCCATGATGTGAGCAGCGCGATTAACCGCCCGCAG
ATTGGCGTGGTGCGCGAATTTCTGACCCGCAACCCGGCGTGGCGCAAAGCGGTGTTTATTAGCCCGTATAACAGCCAGAACGCGGTGGCGAGCAAAATTC
TGGGCCTGCCGACCCAGACCGTGGATAGCAGCCAGGGCAGCGAATATGATTATGTGATTTTTACCCAGACCACCGAAACCGCGCATAGCTGCAACGTGAA
CCGCTTTAACGTGGCGATTACCCGCGCGAAAGTGGGCATTCTGTGCATTATGAGCGATCGCGATCTGTATGATAAACTGCAGTTTACCAGCCTGGAAATT
CCGCGCCGCAACGTGGCGACCCTGCAGGGACCCCATCATCATCATCATCATTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPHTVLQAVGACVLCNSQTSLRCGACIRRPFLCCKCCYDHVISTSHKLVLSVNPYVCNAPGCDVTDVTQL
YLGGMSYYCKSHKPPISFPLCANGQVFGLYKNTCVGSDNVTDFNAIATCDWTNAGDYILANTCTERLKLFAAETLKATEETFKLSYGIATVREVLSDREL
HLSWEVGKPRPPLNRNYVFTGYRVTKNSKVQIGEYTFEKGDYGDAVVYRGTTTYKLNVGDYFVLTSHTVMPLSAPTLVPQEHYVRITGLYPTLNISDEFS
SNVANYQKVGMQKYSTLQGPPGTGKSHFAIGLALYYPSARIVYTACSHAAVDALCEKALKYLPIDKCSRIIPARARVECFDKFKVNSTLEQYVFCTVNAL
PETTADIVVFDEISMATNYDLSVVNARLRAKHYVYIGDPAQLPAPRTLLTKGTLEPEYFNSVCRLMKTIGPDMFLGTCRRCPAEIVDTVSALVYDNKLKA
HKDKSAQCFKMFYKGVITHDVSSAINRPQIGVVREFLTRNPAWRKAVFISPYNSQNAVASKILGLPTQTVDSSQGSEYDYVIFTQTTETAHSCNVNRFNV
AITRAKVGILCIMSDRDLYDKLQFTSLEIPRRNVATLQGPHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P1 SARS-CoV-2 Nsp12 P919-Q924 Nsp13 6His521.62 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU67899
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee