SARS-CoV2 (2019-nCoV) NSP11/12

• Plasmid: pMEX3Cb SARS-CoV-2 NSP11/12
• Parent Plasmid: pMEX3Cb
• Expressed: MBP 3C SARS-CoV-2 NSP11/12
• Genbank: MN908947.3
• Tag: MBP
• Species: SARS-CoV-2

Sequence of Insert

GGATCCAACCGCGTGTGCGGCGTGAGCGCGGCGCGCCTGACCCCGTGCGGCACCGGCACCAGCACCGATGTGGTGTATCGCGCGTTTGATATTTATAACGATAAAGTGGCGGGCTTTGCGAAATTTCTGAAAACCAACTGCTGCCGCTTTCAGGAAAAAGATGAAGATGATAACCTGATTGATAGCTATTTTGTGGTGAAACGCCATACCTTTAGCAACTATCAGCATGAAGAAACCATTTATAACCTGCTGAAAGATTGCCCGGCGGTGGCGAAACATGATTTTTTTAAATTTCGCATTGATGGCGATATGGTGCCGCATATTAGCCGCCAGCGCCTGACCAAATATACCATGGCGGATCTGGTGTATGCGCTGCGCCATTTTGATGAAGGCAACTGCGATACCCTGAAAGAAATTCTGGTGACCTATAACTGCTGCGATGATGATTATTTTAACAAAAAAGATTGGTATGATTTTGTGGAAAACCCGGATATTCTGCGCGTGTATGCGAACCTGGGCGAACGCGTGCGCCAGGCGCTGCTGAAAACCGTGCAGTTTTGCGATGCGATGCGCAACGCGGGCATTGTGGGCGTGCTGACCCTGGATAACCAGGATCTGAACGGCAACTGGTATGATTTTGGCGATTTTATTCAGACCACCCCGGGCAGCGGCGTGCCGGTGGTGGATAGCTATTATAGCCTGCTGATGCCGATTCTGACCCTGACCCGCGCGCTGACCGCGGAAAGCCATGTGGATACCGATCTGACCAAACCGTATATTAAATGGGATCTGCTGAAATATGATTTTACCGAAGAACGCCTGAAACTGTTTGATCGCTATTTTAAATATTGGGATCAGACCTATCATCCGAACTGCGTGAACTGCCTGGATGATCGCTGCATTCTGCATTGCGCGAACTTTAACGTGCTGTTTAGCACCGTGTTTCCGCCGACCAGCTTTGGCCCGCTGGTGCGCAAAATTTTTGTGGATGGCGTGCCGTTTGTGGTGAGCACCGGCTATCATTTTCGCGAACTGGGCGTGGTGCATAACCAGGATGTGAACCTGCATAGCAGCCGCCTGAGCTTTAAAGAACTGCTGGTGTATGCGGCGGAcCCGGCGATGCATGCGGCGAGCGGCAACCTGCTGCTGGATAAACGCACCACCTGCTTTAGCGTGGCGGCGCTGACCAACAACGTGGCGTTTCAGACCGTGAAACCGGGCAACTTTAACAAAGATTTTTATGATTTTGCGGTGAGCAAAGGCTTTTTTAAAGAAGGCAGCAGCGTGGAACTGAAACATTTTTTTTTTGCGCAGGATGGCAACGCGGCGATTAGCGATTATGATTATTATCGCTATAACCTGCCGACCATGTGCGATATTCGCCAGCTGCTGTTTGTGGTGGAAGTGGTGGATAAATATTTTGATTGCTATGATGGCGGCTGCATTAACGCGAACCAGGTGATTGTGAACAACCTGGATAAAAGCGCGGGCTTTCCGTTTAACAAATGGGGCAAAGCGCGCCTGTATTATGATAGCATGAGCTATGAAGATCAGGATGCGCTGTTTGCGTATACCAAACGCAACGTGATTCCGACCATTACCCAGATGAACCTGAAATATGCGATTAGCGCGAAAAACCGCGCGCGCACCGTGGCGGGCGTGAGCATTTGCAGCACCATGACCAACCGCCAGTTTCATCAGAAACTGCTGAAAAGCATTGCGGCGACCCGCGGCGCGACCGTGGTGATTGGCACCAGCAAATTTTATGGCGGCTGGCATAACATGCTGAAAACCGTGTATAGCGATGTGGAAAACCCGCATCTGATGGGCTGGGATTATCCGAAATGCGATCGCGCGATGCCGAACATGCTGCGCATTATGGCGAGCCTGGTGCTGGCGCGCAAACATACCACCTGCTGCAGCCTGAGCCATCGCTTTTATCGCCTGGCGAACGAATGCGCGCAGGTGCTGAGCGAAATGGTGATGTGCGGCGGCAGCCTGTATGTGAAACCGGGCGGCACCAGCAGCGGCGATGCGACCACCGCGTATGCGAACAGCGTGTTTAACATTTGCCAGGCGGTGACCGCGAACGTGAACGCGCTGCTGAGCACCGATGGCAACAAAATTGCGGATAAATATGTGCGCAACCTGCAGCATCGCCTGTATGAATGCCTGTATCGCAACCGCGATGTGGATACCGATTTTGTGAACGAATTTTATGCGTATCTGCGCAAACATTTTAGCATGATGATTCTGAGCGATGATGCGGTGGTGTGCTTTAACAGCACCTATGCGAGCCAGGGCCTGGTGGCGAGCATTAAAAACTTTAAAAGCGTGCTGTATTATCAGAACAACGTGTTTATGAGCGAAGCGAAATGCTGGACCGAAACCGATCTGACCAAAGGCCCGCATGAATTTTGCAGCCAGCATACCATGCTGGTGAAACAGGGCGATGATTATGTGTATCTGCCGTATCCGGAcCCGAGCCGCATTCTGGGCGCGGGCTGCTTTGTGGATGATATTGTGAAAACCGATGGCACCCTGATGATTGAACGCTTTGTGAGCCTGGCGATTGATGCGTATCCGCTGACCAAACATCCGAACCAGGAATATGCGGATGTGTTTCATCTGTATCTGCAGTATATTCGCAAACTGCATGATGAACTGACCGGCCATATGCTGGATATGTATAGCGTGATGCTGACCAACGATAACACCAGCCGCTATTGGGAACCGGAATTTTATGAAGCGATGTATACCCCGCATACCGTGCTGCAGtgaGCGGCCGC

Amino Acid Sequence

MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRY
NGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLI
KNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL
GAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGP
LGSNRVCGVSAARLTPCGTGTSTDVVYRAFDIYNDKVAGFAKFLKTNCCRFQEKDEDDNLIDSYFVVKRHTFSNYQHEETIYNLLKDCPAVAKHDFFKFR
IDGDMVPHISRQRLTKYTMADLVYALRHFDEGNCDTLKEILVTYNCCDDDYFNKKDWYDFVENPDILRVYANLGERVRQALLKTVQFCDAMRNAGIVGVL
TLDNQDLNGNWYDFGDFIQTTPGSGVPVVDSYYSLLMPILTLTRALTAESHVDTDLTKPYIKWDLLKYDFTEERLKLFDRYFKYWDQTYHPNCVNCLDDR
CILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHFRELGVVHNQDVNLHSSRLSFKELLVYAADPAMHAASGNLLLDKRTTCFSVAALTNNV
AFQTVKPGNFNKDFYDFAVSKGFFKEGSSVELKHFFFAQDGNAAISDYDYYRYNLPTMCDIRQLLFVVEVVDKYFDCYDGGCINANQVIVNNLDKSAGFP
FNKWGKARLYYDSMSYEDQDALFAYTKRNVIPTITQMNLKYAISAKNRARTVAGVSICSTMTNRQFHQKLLKSIAATRGATVVIGTSKFYGGWHNMLKTV
YSDVENPHLMGWDYPKCDRAMPNMLRIMASLVLARKHTTCCSLSHRFYRLANECAQVLSEMVMCGGSLYVKPGGTSSGDATTAYANSVFNICQAVTANVN
ALLSTDGNKIADKYVRNLQHRLYECLYRNRDVDTDFVNEFYAYLRKHFSMMILSDDAVVCFNSTYASQGLVASIKNFKSVLYYQNNVFMSEAKCWTETDL
TKGPHEFCSQHTMLVKQGDDYVYLPYPDPSRILGAGCFVDDIVKTDGTLMIERFVSLAIDAYPLTKHPNQEYADVFHLYLQYIRKLHDELTGHMLDMYSV
MLTNDNTSRYWEPEFYEAMYTPHTVLQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pMEX3Cb SARS-CoV-2 NSP11/12 574.24 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU67851
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee