USP26

• Plasmid: pCAGGS puro 3FLAG USP26 L364F
• Parent Plasmid: pCAGGS puro 3FLAG
• Expressed: 3FLAG USP26 L364F
• Genbank: NM_031907.1
• Tag: 3FLAG
• Species: Human

Sequence of Insert

GGATCCATGGCTGCCCTATTCCTACGTGGTTTTGTCCAAATAGGGAACTGCAAGACTGGGATATCTAAGTCAAAAGAAGCATTCATTGAAGCAGTGGAAAGAAAGAAGAAAGATAGACTGGTGCTGTATTTCAAAAGTGGAAAATATAGCACTTTTCGGCTAAGTGATAATATTCAAAATGTAGTCCTTAAATCCTATAGAGGAAACCAAAATCACCTGCATTTAACTTTACAAAATAATAATGGCTTGTTTATTGAAGGATTATCCTCCACAGATGCTGAACAATTGAAGATATTCTTGGACAGAGTTCATCAAAACGAGGTTCAGCCACCTGTGAGACCTGGTAAGGGTGGGAGTGTCTTTTCTAGCACAACACAGAAGGAAATCAACAAAACTTCATTCCACAAAGTTGATGAGAAATCAAGTAGCAAATCTTTTGAGATAGCAAAAGGAAGTGGGACAGGTGTCCTTCAGAGGATGCCTTTGCTTACATCAAAATTGACACTTACTTGCGGAGAGTTATCAGAAAATCAGCACAAGAAGAGGAAAAGAATGCTCTCATCTAGCTCAGAGATGAATGAGGAATTCTTGAAAGAAAATAATTCTGTAGAATACAAGAAATCCAAGGCAGATTGTTCGAGGTGTGTAAGCTATAATCGAGAGAAACAATTGAAGTTAAAAGAGTTAGAAGAGAATAAGAAATTGGAATGTGAATCTTCATGCATCATGAACGCCACTGGAAATCCTTACCTAGATGACATTGGTCTTCTCCAAGCTCTCACTGAGAAAATGGTTTTGGTATTTCTGTTACAACAAGGGTATAGTGACGGTTACACAAAGTGGGATAAATTAAAACTATTTTTTGAATTATTTCCAGAGAAAATATGCCACGGCCTCCCCAATTTGGGAAACACCTGTTATATGAATGCAGTGTTACAGTCTCTACTTTCAATCCCATCGTTTGCTGATGATTTACTTAATCAGAGTTTCCCATGGGGTAAAATTCCCCTTAATGCTCTTACCATGTGCTTGGCACGGCTACTTTTTTTTAAAGATACCTATAATATAGAAATCAAGGAGATGTTACTCTTGAATTTTAAAAAGGCCATTTCAGCAGCTGCAGAGATATTCCATGGCAATGCACAGAACGATGCTCATGAGTTTTTAGCTCACTGTTTAGATCAACTGAAAGATAACATGGAAAAACTCAACACAATTTGGAAGCCTAAAAGTGAATTTGGGGAAGATAATTTTCCTAAACAGGTTTTTGCTGATGATCCTGACACCAGTGGGTTTTCTTGCCCTGTCATTACTAATTTTGAGTTAGAGTTGTTGCACTCCATTGCTTGTAAAGCTTGTGGTCAGGTTATTCTCAAGACAGAACTGAATAATTACCTCTCCATCAACCTTCCCCAAAGAATAAAAGCACATCCTTCATCTATTCAGTCTACTTTTGATCTTTTTTTTGGAGCAGAAGAGCTTGAGTATAAATGTGCAAAATGTGAGCACAAGACTTCCGTTGGAGTGCACTCATTCAGTAGGCTACCTAGAATCCTTATTGTTCACCTCAAACGCTATAGCTTGAATGAGTTTTGTGCATTAAAGAAGAATGACCAGGAAGTCATCATTTCCAAATATTTAAAGGTGTCTTCTCATTGCAATGAAGGCACCAGACCACCTCTTCCCTTGAGTGAGGATGGAGAAATTACAGATTTCCAATTATTAAAAGTTATTCGAAAGATGACTTCTGGAAACATCAGTGTATCATGGCCTGCAACAAAGGAATCCAAAGATATCCTGGCTCCACACATTGGATCAGATAAGGAGTCTGAACAAAAAAAAGGCCAGACAGTCTTTAAAGGGGCAAGCAGAAGACAGCAGCAAAAGTACCTTGGAAAAAATTCTAAACCAAATGAGCTAGAATCTGTATACTCAGGAGATCGAGCATTCATTGAAAAAGAACCGTTAGCTCACTTAATGACGTATCTGGAAGATACCTCACTTTGTCAGTTCCACAAAGCTGGAGGTAAACCTGCCAGCAGCCCAGGCACACCTCTCTCAAAAGTTGACTTTCAAACAGTGCCCGAAAATCCAAAACGAAAGAAATATGTGAAAACCAGTAAGTTTGTAGCTTTTGATAGGATTATCAATCCTACTAAAGATTTGTATGAAGATAAAAATATCAGAATTCCAGAAAGATTCCAAAAAGTGTCTGAACAGACTCAGCAGTGTGACGGTATGAGAATCTGTGAACAAGCCCCTCAGCAGGCACTGCCTCAAAGCTTTCCAAAGCCAGGCACCCAGGGGCACACAAAGAACCTCCTAAGACCTACAAAATTAAATCTACAGAAGTCTAACAGGAATTCCCTACTTGCACTGGGTTCCAATAAGAATCCAAGAAACAAAGACATTTTAGATAAGATAAAATCTAAAGCCAAGGAAACAAAAAGAAATGATGATAAGGGAGATCATACCTACCGGCTCATTAGTGTTGTCAGCCATCTTGGGAAGACTCTAAAGTCAGGCCATTATATCTGTGATGCCTATGACTTTGAGAAACAGATCTGGTTCACTTACGATGATATGCGGGTGTTAGGTATCCAGGAGGCCCAGATGCAGGAGGATAGGCGTTGCACTGGGTACATCTTCTTTTACATGCATAATGAGATCTTTGAAGAGATGTTGAAAAGAGAAGAGAATGCCCAGCTTAATAGCAAGGAGGTAGAGGAGACCCTTCAGAAGGAATAAGCGGCCGC

Amino Acid Sequence

MDYKDHDGDYKDHDIDYKDDDDKGSMAALFLRGFVQIGNCKTGISKSKEAFIEAVERKKKDRLVLYFKSGKYSTFRLSDNIQNVVLKSYRGNQNHLHLTL
QNNNGLFIEGLSSTDAEQLKIFLDRVHQNEVQPPVRPGKGGSVFSSTTQKEINKTSFHKVDEKSSSKSFEIAKGSGTGVLQRMPLLTSKLTLTCGELSEN
QHKKRKRMLSSSSEMNEEFLKENNSVEYKKSKADCSRCVSYNREKQLKLKELEENKKLECESSCIMNATGNPYLDDIGLLQALTEKMVLVFLLQQGYSDG
YTKWDKLKLFFELFPEKICHGLPNLGNTCYMNAVLQSLLSIPSFADDLLNQSFPWGKIPLNALTMCLARLLFFKDTYNIEIKEMLLLNFKKAISAAAEIF
HGNAQNDAHEFLAHCLDQLKDNMEKLNTIWKPKSEFGEDNFPKQVFADDPDTSGFSCPVITNFELELLHSIACKACGQVILKTELNNYLSINLPQRIKAH
PSSIQSTFDLFFGAEELEYKCAKCEHKTSVGVHSFSRLPRILIVHLKRYSLNEFCALKKNDQEVIISKYLKVSSHCNEGTRPPLPLSEDGEITDFQLLKV
IRKMTSGNISVSWPATKESKDILAPHIGSDKESEQKKGQTVFKGASRRQQQKYLGKNSKPNELESVYSGDRAFIEKEPLAHLMTYLEDTSLCQFHKAGGK
PASSPGTPLSKVDFQTVPENPKRKKYVKTSKFVAFDRIINPTKDLYEDKNIRIPERFQKVSEQTQQCDGMRICEQAPQQALPQSFPKPGTQGHTKNLLRP
TKLNLQKSNRNSLLALGSNKNPRNKDILDKIKSKAKETKRNDDKGDHTYRLISVVSHLGKTLKSGHYICDAYDFEKQIWFTYDDMRVLGIQEAQMQEDRR
CTGYIFFYMHNEIFEEMLKREENAQLNSKEVEETLQKE*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCAGGS puro 3FLAG USP26 L364F125.94 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61171
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee