HOIP

• Synonyms: RNF31
• Plasmid: pRetrox tight puro HOIP Q613A/L617A/F620A/R623A
• Parent Plasmid: pRetroX tight puro
• Expressed: HOIP Q613A/L617A/F620A/R623A
• Genbank: NM_017999.5
• Species: Human

Sequence of Insert

GCGGCCGCGATGCCGGGGGAGGAAGAGGAGCGGGCCTTCCTGGTGGCCCG
CGAGGAGCTGGCGAGCGCCCTGAGGAGGGATTCCGGGCAGGCGTTTTCCC
TGGAGCAGCTCCGGCCGCTACTAGCCAGCTCTCTGCCGCTAGCCGCCCGC
TACCTGCAGCTGGACGCCGCACGCCTTGTCCGCTGCAACGCTCATGGGGA
GCCCCGAAACTACCTCAACACCCTGTCCACGGCTCTGAACATCCTGGAGA
AATACGGCCGCAACCTTCTCAGCCCTCAGCGGCCTCGGTACTGGCGTGGT
GTCAAGTTTAATAACCCTGTCTTTCGCAGCACGGTGGATGCTGTGCAGGG
GGGCCGAGATGTGCTGCGATTATATGGCTACACAGAGGAGCAACCAGATG
GGTTGAGCTTCCCCGAAGGGCAGGAGGAGCCAGATGAGCACCAGGTTGCT
ACAGTCACACTGGAAGTACTGCTGCTTCGGACAGAGCTCAGCCTGCTATT
GCAGAATACTCATCCAAGACAGCAGGCACTGGAGCAGCTGTTGGAAGACA
AGGTTGAAGATGATATGCTGCAGCTTTCAGAATTTGACCCCCTATTGAGA
GAGATTGCTCCTGGCCCCCTCACCACACCCTCTGTCCCAGGCTCCACTCC
TGGTCCCTGCTTCCTCTGTGGTTCTGCCCCAGGCACACTGCACTGCCCAT
CCTGTAAACAGGCCCTGTGTCCAGCCTGTGACCACCTGTTCCATGGACAC
CCATCCCGTGCTCATCACCTCCGCCAGACCCTGCCTGGGGTCCTGCAGGG
TACCCACCTGAGCCCCAGTTTACCTGCCTCAGCCCAACCACGGCCCCAGT
CGACCTCCCTGCTGGCCCTGGGAGACAGCTCTCTTTCTTCCCCTAATCCT
GCAAGTGCTCATTTGCCCTGGCACTGTGCTGCCTGTGCCATGCTAAATGA
GCCTTGGGCAGTGCTCTGTGTGGCCTGTGATCGGCCCCGAGGCTGTAAGG
GGTTGGGGTTGGGAACTGAGGGTCCCCAAGGAACTGGAGGCCTAGAACCT
GATCTTGCACGGGGTCGGTGGGCCTGCCAGAGCTGTACCTTTGAGAATGA
GGCAGCTGCTGTGCTATGTTCCATATGTGAGCGACCTCGGCTGGCCCAGC
CTCCCAGCTTGGTGGTGGATTCCCGAGATGCTGGCATTTGCCTGCAACCC
CTTCAGCAGGGGGATGCTTTGCTGGCCTCTGCCCAGAGTCAAGTCTGGTA
CTGTATTCACTGTACCTTCTGCAACTCGAGCCCTGGCTGGGTGTGTGTTA
TGTGCAACCGGACTAGTAGCCCCATTCCAGCACAACATGCCCCCCGGCCC
TATGCCAGCTCTTTGGAAAAGGGACCCCCCAAGCCTGGGCCCCCACGACG
CCTTAGTGCCCCCCTGCCCAGTTCCTGTGGAGATCCTGAGAAGCAGCGCC
AAGACAAGATGCGGGAAGAAGGCCTCCAGCTAGTGAGCATGATCCGGGAA
GGGGAAGCCGCAGGTGCCTGTCCAGAGGAGATCTTCTCGGCTCTGCAGTA
CTCGGGCACTGAGGTGCCTCTGCAGTGGTTGCGCTCAGAACTGCCCTACG
TCCTGGAGATGGTGGCTGAGCTGGCTGGACAGCAGGACCCTGGGCTGGGT
GCCTTTTCCTGTCAGGAGGCCCGGAGAGCCTGGCTGGATCGTCATGGCAA
CCTTGATGAAGCTGTGGAGGAGTGTGTGAGGACCAGGCGAAGGAAGGTGC
AGGAGCTCCAGTCTCTAGGCTTTGGGCCTGAGGAGGGGTCTCTCCAGGCA
TTGTTCCAGCACGGAGGTGATGTGTCACGGGCCCTGACTGAGCTAgcGCG
CCAACGCgcAGAGCCCgcCCGCCAGgctCTCTGGGACAGTGGCCCTGAGC
CCACCCCTTCCTGGGATGGGCCAGACAAGCAGAGCCTGGTCAGGCGGCTT
TTGGCAGTCTACGCACTCCCCAGCTGGGGCCGGGCAGAGCTGGCACTGTC
ACTGCTGCAGGAGACACCCAGGAACTATGAGTTGGGGGATGTGGTAGAAG
CTGTGAGGCACAGCCAGGACCGGGCCTTCCTGCGCCGCTTGCTTGCCCAG
GAGTGTGCCGTGTGTGGCTGGGCCCTGCCCCACAACCGGATGCAGGCCCT
GACTTCCTGTGAGTGCACCATCTGTCCTGACTGCTTCCGCCAGCACTTCA
CCATCGCCTTGAAGGAGAAGCACATCACAGACATGGTGTGCCCTGCCTGT
GGCCGCCCCGACCTCACCGATGACACACAGTTGCTCAGCTACTTCTCTAC
CCTTGACATCCAGCTTCGCGAGAGCCTAGAGCCAGATGCCTATGCGTTGT
TCCATAAGAAGCTGACCGAGGGTGTGCTGATGCGGGACCCCAAGTTCTTG
TGGTGTGCCCAGTGCTCCTTTGGCTTCATATATGAGCGTGAGCAGCTGGA
GGCAACTTGTCCCCAGTGTCACCAGACCTTCTGTGTGCGCTGCAAGCGCC
AGTGGGAGGAGCAGCACCGAGGTCGGAGCTGTGAGGACTTCCAGAACTGG
AAACGCATGAACGACCCAGAATACCAGGCCCAGGGCCTAGCAATGTATCT
TCAGGAAAACGGCATTGACTGCCCCAAATGCAAGTTCTCGTACGCCCTGG
CCCGAGGAGGCTGCATGCACTTTCACTGTACCCAGTGCCGCCACCAGTTC
TGCAGCGGCTGCTACAATGCCTTTTACGCCAAGAATAAATGTCCAGAGCC
TAACTGCAGGGTGAAAAAGTCCCTGCACGGCCACCACCCTCGAGACTGCC
TCTTCTACCTGCGGGACTGGACTGCTCTCCGGCTTCAGAAGCTGCTACAG
GACAATAACGTCATGTTTAATACAGAGCCTCCAGCTGGGGCCCGGGCAGT
CCCTGGAGGCGGCTGCCGAGTGATAGAGCAGAAGGAGGTTCCCAATGGGC
TCAGGGACGAAGCTTGTGGCAAGGAAACTCCAGCTGGCTATGCCGGCCTG
TGCCAGGCACACTACAAAGAGTATCTTGTGAGCCTCATCAATGCCCACTC
GCTGGACCCAGCCACCTTGTATGAGGTGGAAGAGCTGGAGACGGCCACTG
AGCGCTACCTGCACGTACGCCCCCAGCCTTTGGCTGGAGAGGATCCCCCT
GCTTACCAGGCCCGCTTGTTACAGAAGCTGACAGAAGAGGTACCCTTGGG
ACAGAGTATCCCCCGCAGGCGGAAGTAGGCGGCCGC

Amino Acid Sequence

MPGEEEERAFLVAREELASALRRDSGQAFSLEQLRPLLASSLPLAARYLQ
LDAARLVRCNAHGEPRNYLNTLSTALNILEKYGRNLLSPQRPRYWRGVKF
NNPVFRSTVDAVQGGRDVLRLYGYTEEQPDGLSFPEGQEEPDEHQVATVT
LEVLLLRTELSLLLQNTHPRQQALEQLLEDKVEDDMLQLSEFDPLLREIA
PGPLTTPSVPGSTPGPCFLCGSAPGTLHCPSCKQALCPACDHLFHGHPSR
AHHLRQTLPGVLQGTHLSPSLPASAQPRPQSTSLLALGDSSLSSPNPASA
HLPWHCAACAMLNEPWAVLCVACDRPRGCKGLGLGTEGPQGTGGLEPDLA
RGRWACQSCTFENEAAAVLCSICERPRLAQPPSLVVDSRDAGICLQPLQQ
GDALLASAQSQVWYCIHCTFCNSSPGWVCVMCNRTSSPIPAQHAPRPYAS
SLEKGPPKPGPPRRLSAPLPSSCGDPEKQRQDKMREEGLQLVSMIREGEA
AGACPEEIFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGAFS
CQEARRAWLDRHGNLDEAVEECVRTRRRKVQELQSLGFGPEEGSLQALFQ
HGGDVSRALTELARQRAEPARQALWDSGPEPTPSWDGPDKQSLVRRLLAV
YALPSWGRAELALSLLQETPRNYELGDVVEAVRHSQDRAFLRRLLAQECA
VCGWALPHNRMQALTSCECTICPDCFRQHFTIALKEKHITDMVCPACGRP
DLTDDTQLLSYFSTLDIQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCA
QCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRM
NDPEYQAQGLAMYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSG
CYNAFYAKNKCPEPNCRVKKSLHGHHPRDCLFYLRDWTALRLQKLLQDNN
VMFNTEPPAGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQA
HYKEYLVSLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQ
ARLLQKLTEEVPLGQSIPRRRK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65219
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee