PINK1 (Salmon)

• Plasmid: pET15 6His sumo(b) codon optimised Salmon PINK1
• Parent Plasmid: pET15 6HIS SUMO
• Expressed: 6His sumo(b) codon optimised Salmon PINK1
• Genbank: codon optimised XM_014146827.1
• Tag: 6His
• Tag: SUMO
• Species: Salmon

Sequence of Insert

GGA(TCC)ATGAGCGTGAAACATGCAATTAGCCGTGGTGTTGAACTGGGTCGTAGCGTTATTCAGCTGGGTCTGCTGAAACCGGCAGGTCGTCTGGCAGCAAAATTTCGTGGTGAACGTCTGCGTGTTGGTCAGCCTGCACGTACCGTTCAGCCGCAGACCTTTCTGCCGGTTCGTTATCGTTATTTTCGTATGAGCCTGCGTGGCCTGGCAGCACAGCTGCAGAGCGGTGGTTTTCGTCGTTTTGGTGGTAGCGGTAGTCCGCGTAATCGTGCAGTTTTTCTGGCATTTGGTTTAGGTGTTGGTCTGATTGAACAGCAGCTGGATGATGATCGTCGTAGCGCAGCAACCTGTCAAGAAATTCAGGCAGTTTTCACCAAAAAAAACTGCTCACCGCTGAAAAGCTTTACCAGTGGTTATAAACTGGAAGATTATGTGATTGGCAAGCAGATTGGTAAAGGTAGCAATGCAGCAGTTTATGAAGCAGCAGCACCGTTTGCAGTTCCGCGTGATCGCGAAAGCGATCGTTGTAGCCTGAATGATCAGCCGAGTGATGATGGTGAAGTTGCAAATGGTAGCCTGCGTAGCCCGAGCAGCTCACTGTGTATTTATCCGCTGGCAGTTAAAATGATGTGGAATTTTGGTGCAGGTAGCAGCAGCGAAGCAATTCTGCGTAGCATGAGCCAAGAACTGGTTCCTGCAGGTCCGCTGGCCATGAAACAAGAAAAAGAAGAACAAATTGCCCTGAACGGCTATTTTGGCGAAGTTCCGAAACGTGTTAGCGCACATCCGAATGTTATTCGTGTTTTTCGTGCATTTACCGCAGATGTTCCGCTGCTGCCTGGTGCACAAGAGGAATATCCGGATGTTCTGCCAGCACGTCTGAATCCGGAAGGTCTGGGTAATAATCGTACCCTGTTTCTGGTGATGAAAAACTATCCGTGTACACTGCGTCAGTATCTGGAAGTTAATGTTCCGAGCCGTCGTGAAGGTAGCCTGATGGTGCTGCAGCTGCTGGAAGGTGTTGATCATCTGTATCGTCAGGGTATTGCACATCGTGATCTGAAAAGCGATAATGTCCTGCTGGAATTTGATAGCGAAGGTTGTCCGCGTCTGGTTATTACCGATTTTGGTTGTTGTCTGGCACAGAGCGATAGCAGCCTGCAACTGCCGTTTAATACCATGTGGGTTAATCGTGGTGGTAATAGCTGTCTGATGGCACCGGAAGTTGTTACCGCAGTTCCTGGTCAGGGTGTTGTTATTAACTATAGCAAAGCAGATGCATGGGCAGTTGGTGCCATTAGCTATGAAATTTTTGGTCAGGCCAATCCGTTTTATGGTGCCGGTGGTCTGGATAGCCGTAATTTTCAAGAGATTCAGCTGCCTGCACTGCCTGCCTGTGTGAGCGCAGATATGCAGCTGGTTGTTAAACTGTTACTGCGTCGTAATCCGAATAAACGTCCGAGCGCACGTGTTGCAGCCAATATGCTGCATCTGAGCCTGTGGGGTCGTCGTGCACTGGCAAATCAGGATAGCGCAGGTATGAAAAAACTGGCAGATTGGCTGCTGTGGCGTTCAGCAGTTGTTCTGCTGCGTGGTTGTGCACCGAGCGGTAGCAGTGTTGAAGTTGAACTGCAGCGTTGTTTTCTGAGCAATCTGGATCTGGAAGATCTGCGTCTGGCCGTTAGCTTTCTGATGTATGGTCGTGAACAGGGTCGTGCAAGCATTATGAGCCGTTAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMSVKHAISRGVELGRSVIQLGLLKPAGRLAAKFRGERLRVGQPARTVQPQTFLPVRYRYFRMSLRGLAAQLQSGGFRRFGGSGSPRNRAVFLAFGL
GVGLIEQQLDDDRRSAATCQEIQAVFTKKNCSPLKSFTSGYKLEDYVIGKQIGKGSNAAVYEAAAPFAVPRDRESDRCSLNDQPSDDGEVANGSLRSPSS
SLCIYPLAVKMMWNFGAGSSSEAILRSMSQELVPAGPLAMKQEKEEQIALNGYFGEVPKRVSAHPNVIRVFRAFTADVPLLPGAQEEYPDVLPARLNPEG
LGNNRTLFLVMKNYPCTLRQYLEVNVPSRREGSLMVLQLLEGVDHLYRQGIAHRDLKSDNVLLEFDSEGCPRLVITDFGCCLAQSDSSLQLPFNTMWVNR
GGNSCLMAPEVVTAVPGQGVVINYSKADAWAVGAISYEIFGQANPFYGAGGLDSRNFQEIQLPALPACVSADMQLVVKLLLRRNPNKRPSARVAANMLHL
SLWGRRALANQDSAGMKKLADWLLWRSAVVLLRGCAPSGSSVEVELQRCFLSNLDLEDLRLAVSFLMYGREQGRASIMSR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU63414
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee