PINK1 (Chicken)

• Plasmid: pET15b 6His sumo(nb) cod. op. Chicken PINK1
• Parent Plasmid: pET15b 6His SUMO
• Expressed: 6His sumo(nb) cod. op. Chicken PINK1
• Genbank: Codon optimised XM_423139.5
• Tag: 6His
• Tag: SUMO
• Species: Chicken

Sequence of Insert

GGATCCATGCGTAGCGCAAGTCCGGATAGCCTGCGTACCCATCGTGTTCTGCCGACCGATTATGCATGTGCACTGCGTACCACACCGCCTCAGAGTCCGGCACCTCTGCCTCCGCCTCCACCTTTACCGCCTCCGGGTAGCCGTCGTAGTCTGCGTCGTAGCGTTGCAGCAATGCTGCTGCGTGTTCTGTTTGCACGTGCCTTTCGTCTGCTGCCTGGTGCAGCACCGCGTCGTCCGGTTCCGATTCCGGTTCCTCCGCCTGTTCCGAGCGCAGCAAGCCCTCCGAGCCTGCTGAGCCGTCTGCCGTTTGTTCGTTTTCTGAGCCTGCGTCGTCCTGCAGGTCTGGCAGCAATGGCAGTTCGTCGTGCCCGTGCAGGTCCGTGTATGGCACTGGCACTGGGTTTAGCACTGCTGGAACCGCCTCTGGAAGAACAGCGTCGTGCACGCGCAGTTTGTGGTCGTATTCAGACCGTTTTTGTGGGTAAAAACAAAGCACAGAAAGATCCGCTGAGCAGCCTGCGTTGGCAGGGTTTTAAACTGGAAGATTATCTGATTGGTCAGCCGTTAGGTAAAGGTTGTAATGCAGCAGTTTATGAAGCAGCAATTCCGAGCAGTCCGCATCATCAGGGTAGCACCGAAAGCAGCGCACTGCCGGTTAGCGAAGAGGAACCTGCAGATAAATGTCAGCAGCAGGCAGCATTTCCGCTGGCCATTAAAATGATGTGGAATATTAGCGCAGATAGCAGCAGCGAAGCAATTCTGCGTAATATGCATCGTGAACTGATTCCGGCAACACGTACCGCACTGGCAGGCGAATATGGTGCAGTTTTTCATCACCGTGAACATGTGCTGGGTCGTAAACGTCTGCGTCCGCATCCGAACATTATTCAGGTTATTCGTGCATTTACCAGCAGCGTTCCGCTGCTGCCAGGCGCACTGACAGATTATCCGGATGTGCTGCCGGTGAGCCTGAATCCGCGTGGTATTGGTCGTAGTCATACCCTGTTTCTGGTGATGAAGAATTATCCGTGTACACTGTGTCAGTATCTGCGTGATAATAGTCCGGATTCACGTCTGAGCACCATGATGATTCTGCAGCTGCTGGAAGGTGTTGATCATCTGGTTCGTCATCGTATTGCACATCGTGATCTGAAAAGCGATAATATCCTGGTGGAATTTGATAGCGCAGGTTGTCCGCGTCTGGTTATTACCGATTTTGGTTGTTGTCTGGCAGATGATAGCATTGGTCTGCGCCTGCCGTTTACCAGTATTGATATGGATCGTGGTGGTAATAGCAGCCTGATGCCTCCGGAAGTTACCACCGCAAGCGCAGGTCCTGGTATGGTTATTGATTATAGCAAAGCAGATGCATGGGCAGTTGGTGCAATTGCCTATGAAATTCTGGGTCTGCCGAATCCGTTTTATAGCTGTGGTGATAGCTTTCTGGAAAGCCGTAGCTATCGTGAAGAAGAACTGCCGTCACTGCCGAATGGTGTTCCGTGTGAAGTTAAACAGGTGATTCGTATGCTGTTACAGCGTGATCCGAATAAACGCCTGAGCGCACGTGTTGCAGCCAATGTTCTGCATCTGAGTCTGTGGGGTGAAAGCATTCTGGCAAGCGAAGCACTGAAACCGGATCAGATGACCGCATGGCTGCTGTGTCAGAGCGCAGCGACCCTGCTGATGGATGGTCTGGTTGATAAAAGCCGTGTTGAAACCAAAATGAAGATGTGCTTTCTGGCCAACCTGGATTTTGAAGATCTGTGGACCGCAGTGTTTCTGCTGCTGGCATGGCGTAATCAGAGCGGTTAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSMRSASPDSLRTHRVLPTDYACALRTTPPQSPAPLPPPPPLPPPGSRRSLRRSVAAMLLRVLFARAFRLLPGAAPRRPVPIPVPPPVPSAASPPSL
LSRLPFVRFLSLRRPAGLAAMAVRRARAGPCMALALGLALLEPPLEEQRRARAVCGRIQTVFVGKNKAQKDPLSSLRWQGFKLEDYLIGQPLGKGCNAAV
YEAAIPSSPHHQGSTESSALPVSEEEPADKCQQQAAFPLAIKMMWNISADSSSEAILRNMHRELIPATRTALAGEYGAVFHHREHVLGRKRLRPHPNIIQ
VIRAFTSSVPLLPGALTDYPDVLPVSLNPRGIGRSHTLFLVMKNYPCTLCQYLRDNSPDSRLSTMMILQLLEGVDHLVRHRIAHRDLKSDNILVEFDSAG
CPRLVITDFGCCLADDSIGLRLPFTSIDMDRGGNSSLMPPEVTTASAGPGMVIDYSKADAWAVGAIAYEILGLPNPFYSCGDSFLESRSYREEELPSLPN
GVPCEVKQVIRMLLQRDPNKRLSARVAANVLHLSLWGESILASEALKPDQMTAWLLCQSAATLLMDGLVDKSRVETKMKMCFLANLDFEDLWTAVFLLLA
WRNQSG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU63328
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee