PINK1 (Python)

• Plasmid: pET15b 6His sumo(nb) cod. op. Python PINK1
• Parent Plasmid: pET15b 6His SUMO
• Expressed: 6His sumo(nb) cod. op. Python PINK1
• Genbank: Codon optimised XM_007423116.3
• Tag: 6His
• Tag: SUMO
• Species: Python

Sequence of Insert

GGATCCATGGCAGCCGGTGGTGAAGGTAGCCAGCGTGTTGCAGCAAGTCCGCTGTGGCCTGAAGTTATTGAAGCAAGCAGCCCGTTTGGTGTTACCGCACGTACCCGTTATTTTAGCAGCTTTCCGGTTCAGACCATTTTTATCCAGCGTAATAAACCGCAGAAAGATACCCTGGGTCTGCTGGCACAGCGTGGTTTTAAACTGGAAGAATATTTCATTGGTCAGAGCATTGGTAAAGGTTGTAGCGGTGCAGTTTATGAAGCAGCAGCACCGGCAACACCAGCCGGTGATGGTGATGAACATGATGGTGCAAGCGGTGTTCAGGGTGTTGCAGAAGCAGAAGGTTGGAAAGAAGGTTTTCCGCTGGCCATTAAAATGATGTGGAATATTAGCGCAGGTAGCAGCAGCGAAGGTATTCTGACCACCATGAGCCAAGAACTGGTTCCGGCAAGCGGTCGTGCACTGAGCGGTGAATTTGGTGCAGTTGTTCGTCCGCGTAAACATGTGTTTGGTAAAAAATGGTTAAAGCCGCATCCGAACATTATTCAGGTTATTCGTGCATTTACCAGCCATGTTCCGCTGCTGCCTGGTGCAACCGTTGATTATCCGGATGTTCTGCCGAGCACACTGAATCCGAGCGGTATTGGTCATAGTCGTACCCTGTTTCTGGTCATGAAAAACTATCCGTGTACACTGCGTCAGTATCTGAGCGAAGGTCGTCCGACACCGCGTCTGGGTGCCGTTATGATTCTGCAGCTGCTGGAAGGTGTTGATCATCTGATTCGTCAGGGCGTTGCACATCGTGATCTGAAAAGCGATAATATCCTGGTGGAATTTGATAGTGCAGGTTGTCCTGGTCTGGTTATTACCGATTTTGGTTGTTGTCTGGCAGATGAGAAACTGGGTCTGAAACTGCCGTTTCCGAGCTGGTATGTTGATCGTGGTGGTAATACCTGTCTGATGGCACCGGAAGTTGCAACCGCAGTTCCTGGTCCGGGTGTTGTTATTGATTATACCAAAGCAGATGTTTGGGCAGTTGGTGCACTGGCATATGAAATTCTGGGTGCACGTAATCCGTTTTATGGTCAAGGTGGTGCAGCACTGGATAGCCGTAGCTATCGTGAAGAAGAACTGCCTCCGCTGCCGAGTCCTGTTGCTCCGGAAGTTAAAACCCTGGTTGCAATGCTGCTGCGTCGTAGTCCGGCAAAACGTCCGAGCGCACGTGTTGCCGCAAATGTTCTGCATCTGAGCCTGTGGGCAGAACCAGGTCTGGGTCGTCCGGATCTGTGTCTGCGTCAGCTGGTTGCCTGGCTGCTGCGCCAGAGTGCAGCAGCCCTGCTGACCGATCGTCTGGGTAGCGATCGCGGTGTTGAAAGCCGTCTGCGTCGTGGTTTTCTGGCAAATCTGGAATATGATGATCTGCGTGAAGCTGCCGCACTGCTGTGTAGCGCACCTGGTGGTGGCCAGCTGAGTCCGGCACGTCATTAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSMAAGGEGSQRVAASPLWPEVIEASSPFGVTARTRYFSSFPVQTIFIQRNKPQKDTLGLLAQRGFKLEEYFIGQSIGKGCSGAVYEAAAPATPAGD
GDEHDGASGVQGVAEAEGWKEGFPLAIKMMWNISAGSSSEGILTTMSQELVPASGRALSGEFGAVVRPRKHVFGKKWLKPHPNIIQVIRAFTSHVPLLPG
ATVDYPDVLPSTLNPSGIGHSRTLFLVMKNYPCTLRQYLSEGRPTPRLGAVMILQLLEGVDHLIRQGVAHRDLKSDNILVEFDSAGCPGLVITDFGCCLA
DEKLGLKLPFPSWYVDRGGNTCLMAPEVATAVPGPGVVIDYTKADVWAVGALAYEILGARNPFYGQGGAALDSRSYREEELPPLPSPVAPEVKTLVAMLL
RRSPAKRPSARVAANVLHLSLWAEPGLGRPDLCLRQLVAWLLRQSAAALLTDRLGSDRGVESRLRRGFLANLEYDDLREAAALLCSAPGGGQLSPARH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU63327
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee