PINK1 (tribolium)

• Plasmid: pMEX3C Pink1 (tribolium) E217K
• Parent Plasmid: pMEX3C
• Expressed: MBP Pink1 (tribolium) E217K
• Tag: MBP
• Species: Tribolium castaneum

Sequence of Insert

GGATCCATGAGCGTCCGTGCAGTTGGCAGCCGCCTGTTCAAACATGGCCGTAGCCTGATCCAGCAGTTCTGTAAACGTGACCTGAATACCACCATTGGCG
ATAAAATCAATGCCGTGAGCCAGGCAACCGCCGCACCGAGCTCTCTGCCGAAAACCCAAATTCCGAAAAACTTTGCCCTGCGTAATGTCGGCGTGCAGCT
GGGTCTGCAAGCACGTCGCATTCTGATCGATAACGTCCTGAATCGTGTGACCAATTCACTGTCGGCCGAACTGCGCAAAAAAGCAACGCGTCGCATCCTG
TTCGGCGACAGCGCACCGTTTTTCGCTCTGGTTGGTGTCAGTATTGCCTCCGGCACCGGTATCCTGACGAAAGAAGAAGAACTGGAAGGCGTTTGCTGGG
AAATCCGTGAAGCGATCTCTAAAATCAAATGGCAGTACTACGATATCGACGAAAGCCGCTTTGAATCTAACCCGATCACCCTGAATGATCTGAGTCTGGG
TAAACCGATCGCCAAAGGCACGAACGGTGTGGTTTACTCCGCAAAAGTCAAAGATGACGAAACCGATGACAATAAATATCCGTTTGCCCTGAAAATGATG
TTCAACTACGATATTCAGAGCAATTCTATGGAAATCCTGAAAGCTATGTATCGTAAAACCGTTCCGGCGCGCATGTATTACTCAAACCACGACCTGAACA
ATTGGGAAATTGAACTGGCTAACCGTCGCAAACATCTGCCGCCGCACCCGAATATTGTGGCAATCTTTAGCGTTTTCACCGATCTGATCCAAGAACTGGA
AGGTTCTAAAGACCTGTATCCGGCAGCGCTGCCGCCGCGTCTGCATCCGGAAGGTGAAGGTCGCAACATGAGCCTGTTTCTGCTGATGAAACGTTATGAT
TGTAATCTGCAGTCATTCCTGAGCACCGCTCCGAGTACCCGCACCTCCCTGCTGCTGCTGGCCCAACTGCTGGAAGGCGTGGCGCATATGACGGCCCACG
GTATTGCACATCGTGATCTGAAATCAGACAACCTGCTGCTGGATACCTCAGAACCGGAATCGCCGATTCTGGTTATCTCGGATTTTGGCTGCTGTCTGGC
GGACAAAACCAACGGTCTGAGCCTGCCGTATACGTCTTACGAAATGGATAAAGGCGGTAATACGGCGCTGATGGCCCCGGAAATTATCTGCCAGAAACCG
GGCACCTTTAGTGTCCTGAATTATTCCAAAGCTGACCTGTGGGCGGTGGGTGCAATCGCTTACGAAATCTTCAACTGTCACAATCCGTTCTACGGCCCGT
CTCGCCTGAAAAACTTCAACTACAAAGAAGGTGATCTGCCGAAACTGCCGGACGAAGTGCCGACCGTTATTCAGGCACTGGTGGCTAACCTGCTGAAACG
TAACCCGAATAAACGCCTGGACCCGGAAGTTGCGGCCAATGTCTGCCAGCTGTTTCTGTGGGCACCGAGTACCTGGCTGAAACCGGGTCTGAAAGTTCCG
ACGTCAGGTGAAATTCTGCAATGGCTGCTGTCGCTGACCACGAAAGTCCTGTGCGAAGGCAAAATCAACAACAAATCCTTCGGTGAAAAATTCACCCGTA
ATTGGCGTCGCACGTATCCGGAATACCTGCTGATTAGTTCCTTCCTGTGTCGCGCGAAACTGGCTAATGTTCGTAATGCTCTGCACTGGATTCAAGAAAA
CCTGCCGGAACTGGATTAAGCGGCCGC

Amino Acid Sequence

MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRY
NGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLI
KNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL
GAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGP
LGSMSVRAVGSRLFKHGRSLIQQFCKRDLNTTIGDKINAVSQATAAPSSLPKTQIPKNFALRNVGVQLGLQARRILIDNVLNRVTNSLSAELRKKATRRI
LFGDSAPFFALVGVSIASGTGILTKEEELEGVCWEIREAISKIKWQYYDIDESRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFALKM
MFNYDIQSNSMEILKAMYRKTVPARMYYSNHDLNNWEIELANRRKHLPPHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFLLMKRY
DCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLLDTSEPESPILVISDFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPEIICQK
PGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDLPKLPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLKPGLKV
PTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYLLISSFLCRAKLANVRNALHWIQENLPELD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU56501
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee