Plasmid
pCMV5 Flag LRRK2 mouse T498A D2017A
Parent Plasmid
pCMV5 FLAG
Expressed
Flag LRRK2 mouse T498A D2017A
Tag
FLAG
Species
Mouse
Sequence of Insert
View Sequence
GGATCCACTAGTATGGCCAGTGGCGCCTGTCAGGGCTGCGAAGAGGAAGAGGAGGAGGAGGCTCTGAAGAAGTTGATAGTCAGGCTGAATAATGTCCAGGAAGGCAAGCAGATCGAGACGTTGCTTCAGCTCCTGGAGGACATGCTGGTGTTCACCTACTCGGACCGCGCCTCCAAGTTATTTGAAGATAAAAATTTCCACGTGCCTCTGTTGATTGTCCTGGACTCCTACATGAGAGTTGCCAGTGTACAGCAGGCGGGGTGGTCACTTCTGTGCAAATTAATAGAAGTCTGTCCAGGGACATTGCAAAGCTTAATAGGACCCCAGGATATTGGAAATGATTGGGAAGTCCTTGGTATTCACCGGCTGATTCTTAAAATGTTAACTGTTCATCACGCCAATGTAAACCTGTCAATAGTTGGACTAAAAGCCTTGGATCTCCTCCTAGATTCAGGTAAACTCACCTTGCTGATACTGGATGAAGAATGTGATATTTTCTTGTTAATTTTTGATGCCATGCACAGATATTCAGCCAATGATGAAGTCCAAAAACTGGGATGCAAAGCTTTACACGTGCTTTTTGAGAGAGTTTCCGAGGAACAGCTGACTGAGTTTGTGGAGAACAAAGATTACACGATACTGCTGAGTACGTTCGGCAGCTTCAGAAGGGACAAGGAGATTGTGTACCACGTACTTTGCTGCTTGCATTCCCTGGCGGTTACATGCAGCAATGTAGAGGTCCTCATGAGTGGGAATGTCCGGTGCTACAATCTTGTGGTGGAGGCCATGAAAGCCTTCCCCACCAATGAAAACATCCAAGAGGTGAGCTGCTCCTTGTTCCAGAAGCTTACATTAGGTAACTTTTTCAACATCCTGGTGTTGAACGAAGTGCATGTCTTTGTGGTGAAAGCGGTCCGACAGTATCCTGAGAACGCAGCCTTACAGATCTCTGCACTCAGCTGTTTAGCACTCCTCACTGAGACTATTTTCTTAAACCAAGACTTGGAGGAAAGAAGTGAGACTCAAGAGCAGAGCGAAGAGGAAGACAGTGAGAAGCTTTTCTGGCTGGAACCCTGCTATAAAGCCCTGGTGCGCCATCGAAAGGACAAACACGTGCAGGAGGCTGCCTGCTGGGCACTAAATAACCTCCTTATGTACCAGAACAGTTTGCATGAGAAGATCGGAGATGAAGATGGCCAGTTCCCTGCGCACAGGGAAGTGATGCTGTCTATGCTGATGCACTCTTCTTCCAAAGATGTCTTCCAAGCAGCTGCACATGCTCTGTCCACTCTCTTGGAACAAAATGTTAATTTCAGGAAAATCCTGCTGGCAAAAGGAGTATACCTGAATGTCTTGGAATTGATGCAGAAGCATGCCCATGCGCCTGAGGTGGCAGAGAGTGGCTGCAAGATGCTGAGTCACCTGTTTGAAGGAAGTAACCCTTCTCTGGATACAATGGCAGCAGTGGTCCCTAAAATACTAACAGTGATGAAAGCCCACGGAGCGTCTCTGTCAGTCCAGCTGGAGGCGCTGCGAGCTATCTTGCATTTCGTTGTGCCAGGACTATTGGAAGAATCCAGGGAGGACTCTCAATGCAGACCAAATGTGCTCAGAAAACAGTGTTTCAGGACTGACATCCACAAGCTGGTTCTAGTCGCTCTGAACAGGTTCATTGGGAATCCTGGGATTCAGAAATGTGGATTGAAAGTAATCTCTTCTCTCGCGCACCTTCCTGATGCCACAGAGACATTGTCCCTGCAAGGAGCAGTTGACTCAGTCCTCCACACCTTACAGATGTATCCAGATGACCAAGAAATTCAGTGTCTGGGCTTACACCTTATGGGATGCTTGATGACAAAGAAGAATTTCTGCATAGGGACAGGGCACCTCCTGGCAAAAATTCTGGCTTCCACTTTGCAGCGCTTTAAAGATGTTGCTGAGGTGCAGACTACAGGATTACAGACAACCCTGTCAATACTTGAGCTGTCAGTATCTTTCTCCAAGCTGCTAGTGCACTATTCCTTTGATGTGGTGATATTTCATCAGATGTCTTCCAGTGTTGTAGAACAAAAGGATGAGCAGTTCCTCAATCTATGTTGCAAATGCTTTGCAAAAGTGGCCGTGGATGATGAGCTGAAAAACACCATGCTAGAGAGAGCCTGCGATCAGAATAACAGCATCATGGTTGAATGTTTGCTCCTCTTGGGAGCTGATGCCAACCAAGTGAAGGGGGCAACTTCTTTAATCTATCAGGTATGTGAGAAAGAGAGCAGTCCTAAATTGGTGGAACTGTTGCTTAATGGTGGTTGTCGTGAACAAGATGTACGGAAGGCCCTGACCGTAAGCATCCAAAAGGGCGACAGCCAGGTCATCAGCTTGCTCCTCAGGAAACTTGCCCTGGACCTGGCCAACAACAGCATTTGCCTTGGAGGATTTGGCATAGGAAAAATTGATCCTTCTTGGCTTGGTCCTTTATTTCCAGATAAGTCATCCAATTTAAGGAAGCAAACAAACACAGGATCTGTCCTAGCGAGGAAAGTGCTCCGGTATCAGATGAGAAACACCCTTCAAGAAGGCGTGGCCTCAGGCAGTGACGGCAAGTTTTCTGAAGACGCGCTGGCGAAATTTGGAGAATGGACCTTTATTCCCGACTCTTCTATGGACAGTGTGTTTGGCCAGAGCGATGATCTGGATAGCGAAGGCAGCGAGAGCTCATTTCTCGTGAAGAGGAAGTCCAACTCAATTAGTGTAGGGGAAGTTTACAGAGATCTAGCTCTGCAGCGCTGCTCACCAAATGCTCAGAGGCATTCCAATTCGCTGGGTCCTGTTTTTGACCATGAAGACTTACTGAGACGAAAAAGAAAAATACTGTCTTCAGATGAGTCTCTCAGGTCCTCAAGGCTGCCGTCCCATATGAGGCAATCAGATAGCTCTTCTTCCCTGGCTTCTGAGAGAGAACACATCACGTCGTTAGACCTATCTGCCAACGAACTCAAAGATATTGATGCTCTGAGCCAGAAGTGTTGCCTCAGTAGCCACCTGGAACATCTCACCAAACTGGAACTTCACCAGAATTCACTCACGAGCTTCCCACAGCAGCTGTGTGAGACTCTGAAGTGTTTGATACACTTGGATTTGCACAGTAACAAATTCACCTCATTTCCCTCTTTCGTGTTGAAAATGCCACGTATCACCAACCTAGATGCCTCTCGAAATGACATCGGGCCAACAGTAGTTTTAGACCCTGCGATGAAGTGTCCAAGCCTCAAACAGTTGAATCTGTCCTATAACCAGCTCTCTTCAATCCCAGAGAATCTTGCCCAAGTGGTGGAGAAACTTGAGCAGCTCCTACTGGAAGGAAATAAAATATCCGGGATTTGCTCTCCCCTGAGCCTGAAGGAACTGAAGATTTTAAATCTTAGTAAAAATCACATTCCATCCCTACCTGGAGATTTTCTTGAGGCTTGTTCAAAAGTCGAGAGTTTCAGTGCTCGCATGAATTTTCTTGCTGCAATGCCTGCCTTACCTTCTTCCATAACGAGCTTAAAATTGTCTCAGAACTCTTTCACGTGCATTCCAGAAGCGATTTTCAGTCTTCCGCACTTGCGGTCCTTGGATATGAGCCACAACAACATTGAATGTCTGCCGGGACCTGCACATTGGAAGTCTCTGAACTTAAGGGAACTCATTTTTAGCAAGAATCAGATCAGCACCTTAGACTTTAGTGAGAACCCACACGTGTGGTCAAGAGTAGAGAAACTGCATCTCTCTCATAATAAACTGAAAGAGATTCCTCCAGAAATTGGCTGCCTTGAAAATCTGACGTCTCTCGACGTCAGTTACAACTTGGAACTGAGGTCCTTCCCAAATGAAATGGGGAAGTTGAGCAAGATATGGGATCTTCCCTTGGACGGACTGCATCTGAATTTTGACTTTAAGCACGTAGGATGCAAGGCCAAAGACATCATAAGGTTTCTACAACAACGTCTGAAAAAGGCTGTACCCTACAACCGAATGAAGCTCATGATTGTGGGAAATACGGGGAGCGGTAAGACCACTTTACTGCAACAACTCATGAAAATGAAGAAACCAGAACTTGGCATGCAGGGTGCCACAGTCGGCATAGACGTGCGAGACTGGTCCATCCAAATACGGGGCAAAAGGAGAAAGGACCTGGTTCTAAACGTGTGGGATTTTGCAGGTCGTGAGGAATTCTACAGCACTCACCCCCACTTCATGACCCAGAGAGCCCTCTACCTGGCTGTCTATGATCTCAGCAAGGGGCAGGCAGAGGTGGACGCCATGAAGCCCTGGCTCTTCAATATCAAGGCTCGTGCCTCTTCTTCCCCGGTGATTCTGGTGGGCACACATTTGGATGTTTCTGATGAGAAGCAGCGGAAAGCGTGCATAAGCAAAATCACGAAGGAACTCCTAAATAAGCGAGGATTCCCCACCATCCGGGACTACCACTTTGTGAATGCCACCGAGGAGTCAGATGCGCTGGCAAAGCTTCGGAAAACCATCATAAATGAGAGCCTTAATTTCAAGATCCGAGATCAGCCTGTGGTTGGGCAGCTAATTCCAGATTGCTACGTAGAACTGGAGAAAATCATTTTATCAGAGCGGAAAGCTGTGCCGACTGAGTTTCCTGTGATTAACCGGAAACACCTGTTACAGCTCGTGAACGAACATCAGCTGCAGCTGGATGAGAACGAGCTCCCACACGCCGTTCACTTCCTAAATGAGTCGGGAGTTCTTCTGCATTTTCAAGACCCTGCCCTGCAGCTAAGTGACCTGTACTTTGTGGAACCCAAGTGGCTTTGTAAAGTCATGGCACAGATCTTGACAGTGAAGGTAGACGGCTGTCTGAAACATCCTAAGGGCATCATTTCCCGGAGAGATGTGGAAAAATTCCTTTCAAAGAAGAAGCGATTCCCGAAGAACTATATGATGCAATACTTTAAACTATTAGAAAAATTTCAGATCGCATTGCCAATAGGGGAAGAATATCTTCTGGTTCCAAGCAGCTTGTCTGACCACAGGCCAGTGATAGAGCTCCCCCACTGTGAGAACTCTGAGATCATCATCCGGCTGTACGAAATGCCGTACTTTCCCATGGGATTTTGGTCAAGATTGATTAACCGATTACTTGAAATCTCACCCTTCATGCTTTCTGGCAGAGAGAGAGCACTACGCCCTAACAGGATGTATTGGCGGCAAGGCATCTACTTGAATTGGTCTCCAGAAGCATACTGTCTGGTAGGCTCTGAAGTCTTAGACAATCGACCTGAGAGTTTCTTGAAAATCACAGTTCCGTCTTGTAGAAAAGGTTGTATTCTTCTGGGCCGAGTTGTGGATCATATTGACTCACTCATGGAAGAATGGTTTCCCGGGTTACTGGAGATTGACATTTGTGGGGAAGGAGAAACTCTGTTGAAGAAATGGGCATTGTACAGTTTTAATGATGGTGAAGAACATCAGAAGATCTTGCTTGATGAGTTGATGAAGAAGGCTGAAGAAGGAGACCTGTTAATAAATCCAGACCAACCAAGGCTCACTATTCCAATATCCCAGATTGCTCCGGACTTGATCTTGGCTGACCTGCCTAGAAATATCATGTTGAACAATGATGAGTTGGAATTTGAGGAAGCACCAGAGTTTCTCTTAGGCGATGGAAGTTTTGGATCCGTTTATCGAGCTGCCTACGAAGGAGAGGAAGTGGCTGTGAAGATTTTTAATAAGCACACATCTCTTAGGCTGTTAAGACAAGAGTTGGTGGTCCTTTGTCACCTTCACCACCCCAGCCTGATATCCTTGCTGGCGGCTGGTATTCGTCCTCGGATGTTGGTAATGGAGTTGGCCTCCAAAGGTTCCTTGGATCGCCTGCTGCAGCAGGACAAAGCCAGCCTCACCAGAACCCTCCAGCACAGGATCGCGTTGCATGTGGCCGACGGCCTGAGGTATCTCCACTCAGCCATGATTATTTACCGTGACCTGAAGCCCCACAATGTGCTGCTTTTTACCCTGTATCCCAATGCTGCCATCATTGCGAAGATTGCGGCCTACGGGATCGCACAGTACTGCTGCAGGATGGGAATAAAGACATCAGAGGGCACCCCAGGGTTCCGGGCACCTGAAGTTGCCAGGGGGAATGTCATTTATAACCAACAGGCCGATGTTTATTCTTTTGGCTTACTACTTCACGATATTTGGACAACTGGGAGTAGGATTATGGAGGGTTTGAGGTTCCCAAATGAGTTTGATGAGTTAGCCATACAAGGGAAGTTGCCAGATCCAGTTAAAGAATATGGCTGTGCCCCATGGCCTATGGTTGAGAAGTTAATTACAAAGTGTTTGAAAGAAAATCCTCAAGAAAGACCCACTTCTGCCCAGGTCTTTGACATTTTGAATTCGGCTGAATTAATTTGCCTCATGCGACACATTTTAATACCTAAGAACATCATTGTTGAATGCATGGTTGCCACGAATCTCAATAGCAAGAGTGCGACTCTCTGGTTGGGATGTGGGAACACAGAAAAAGGACAGCTTTCCTTATTTGACTTAAACACGGAAAGATACAGCTATGAGGAAGTTGCTGATAGTAGAATACTGTGCTTGGCTTTGGTGCATCTCGCTGCTGAGAAAGAGAGCTGGGTTGTGTGCGGGACACAGTCTGGGGCTCTCCTGGTCATCAATGTTGAAGAGGAGACAAAGAGACACACCCTGGAAAAGATGACTGATTCTGTCACTTGTTTGCATTGCAATTCCCTTGCCAAGCAGAGCAAGCAAAGTAACTTTCTTTTGGTGGGAACTGCTGATGGTAACTTAATGATATTTGAAGATAAAGCCGTTAAGTGTAAAGGAGCTGCCCCCTTGAAGACACTACACATAGGCGATGTCAGTACGCCCCTGATGTGCCTGAGCGAGTCCCTGAATTCATCTGAAAGACACATCACATGGGGAGGGTGTGGCACAAAGGTCTTCTCCTTTTCCAATGATTTCACCATTCAGAAACTCATCGAGACAAAAACCAACCAGCTGTTTTCTTACGCAGCTTTCAGCGATTCTAACATCATAGCGCTGGCAGTAGACACAGCCCTGTATATTGCCAAGAAAAACAGCCCTGTCGTAGAGGTGTGGGACAAGAAAACAGAAAAGCTCTGTGAATTAATAGACTGTGTGCACTTCTTAAAGGAGGTGATGGTAAAACTAAACAAGGAATCGAAACATCAGCTGTCCTACTCTGGGAGGGTGAAGGCCCTCTGCCTGCAGAAGAACACGGCTCTCTGGATCGGAACTGGAGGAGGCCACATCTTACTCCTGGATCTTTCTACTCGGCGAGTTATCCGCACCATTCACAATTTCTGTGATTCTGTGAGAGCCATGGCCACAGCACAATTAGGAAGCCTTAAGAATGTCATGCTGGTTTTGGGGTACAAGCGGAAGAGTACAGAGGGTATCCAAGAACAAAAAGAGATACAATCTTGTTTGTCTATTTGGGACCTCAATCTTCCACACGAAGTGCAAAATTTAGAAAAACACATTGAAGTAAGAACAGAATTAGCTGATAAAATGAGGAAAACATCTGTTGAATAGCGGCCGC
Amino Acid Sequence
View Sequence
MDYKDDDDKGSTSMASGACQGCEEEEEEEALKKLIVRLNNVQEGKQIETLLQLLEDMLVFTYSDRASKLFEDKNFHVPLLIVLDSYMRVASVQQAGWSLLCKLIEVCPGTLQSLIGPQDIGNDWEVLGIHRLILKMLTVHHANVNLSIVGLKALDLLLDSGKLTLLILDEECDIFLLIFDAMHRYSANDEVQKLGCKALHVLFERVSEEQLTEFVENKDYTILLSTFGSFRRDKEIVYHVLCCLHSLAVTCSNVEVLMSGNVRCYNLVVEAMKAFPTNENIQEVSCSLFQKLTLGNFFNILVLNEVHVFVVKAVRQYPENAALQISALSCLALLTETIFLNQDLEERSETQEQSEEEDSEKLFWLEPCYKALVRHRKDKHVQEAACWALNNLLMYQNSLHEKIGDEDGQFPAHREVMLSMLMHSSSKDVFQAAAHALSTLLEQNVNFRKILLAKGVYLNVLELMQKHAHAPEVAESGCKMLSHLFEGSNPSLDTMAAVVPKILTVMKAHGASLSVQLEALRAILHFVVPGLLEESREDSQCRPNVLRKQCFRTDIHKLVLVALNRFIGNPGIQKCGLKVISSLAHLPDATETLSLQGAVDSVLHTLQMYPDDQEIQCLGLHLMGCLMTKKNFCIGTGHLLAKILASTLQRFKDVAEVQTTGLQTTLSILELSVSFSKLLVHYSFDVVIFHQMSSSVVEQKDEQFLNLCCKCFAKVAVDDELKNTMLERACDQNNSIMVECLLLLGADANQVKGATSLIYQVCEKESSPKLVELLLNGGCREQDVRKALTVSIQKGDSQVISLLLRKLALDLANNSICLGGFGIGKIDPSWLGPLFPDKSSNLRKQTNTGSVLARKVLRYQMRNTLQEGVASGSDGKFSEDALAKFGEWTFIPDSSMDSVFGQSDDLDSEGSESSFLVKRKSNSISVGEVYRDLALQRCSPNAQRHSNSLGPVFDHEDLLRRKRKILSSDESLRSSRLPSHMRQSDSSSSLASEREHITSLDLSANELKDIDALSQKCCLSSHLEHLTKLELHQNSLTSFPQQLCETLKCLIHLDLHSNKFTSFPSFVLKMPRITNLDASRNDIGPTVVLDPAMKCPSLKQLNLSYNQLSSIPENLAQVVEKLEQLLLEGNKISGICSPLSLKELKILNLSKNHIPSLPGDFLEACSKVESFSARMNFLAAMPALPSSITSLKLSQNSFTCIPEAIFSLPHLRSLDMSHNNIECLPGPAHWKSLNLRELIFSKNQISTLDFSENPHVWSRVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDGLHLNFDFKHVGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKMKKPELGMQGATVGIDVRDWSIQIRGKRRKDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACISKITKELLNKRGFPTIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQPVVGQLIPDCYVELEKIILSERKAVPTEFPVINRKHLLQLVNEHQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKVMAQILTVKVDGCLKHPKGIISRRDVEKFLSKKKRFPKNYMMQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPFMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNRPESFLKITVPSCRKGCILLGRVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDELMKKAEEGDLLINPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEEAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIAAYGIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLHDIWTTGSRIMEGLRFPNEFDELAIQGKLPDPVKEYGCAPWPMVEKLITKCLKENPQERPTSAQVFDILNSAELICLMRHILIPKNIIVECMVATNLNSKSATLWLGCGNTEKGQLSLFDLNTERYSYEEVADSRILCLALVHLAAEKESWVVCGTQSGALLVINVEEETKRHTLEKMTDSVTCLHCNSLAKQSKQSNFLLVGTADGNLMIFEDKAVKCKGAAPLKTLHIGDVSTPLMCLSESLNSSERHITWGGCGTKVFSFSNDFTIQKLIETKTNQLFSYAAFSDSNIIALAVDTALYIAKKNSPVVEVWDKKTEKLCELIDCVHFLKEVMVKLNKESKHQLSYSGRVKALCLQKNTALWIGTGGGHILLLDLSTRRVIRTIHNFCDSVRAMATAQLGSLKNVMLVLGYKRKSTEGIQEQKEIQSCLSIWDLNLPHEVQNLEKHIEVRTELADKMRKTSVE*
Vector Type
Mammalian
Antibiotic
Amp
DU Number
DU27484
Unit Source
Location
PPU
MTA
Price
£125.00
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