VPS35 I241M

• Plasmid: pcDNA5 FRT/TO HA VPS35 I241M
• Parent Plasmid: pcDNA5 FRT/TO HA
• Expressed: HA VPS35 I241M
• Genbank: Q96QK1
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGCCTACAACACAGCAGTCCCCTCAGGATGAGCAGGAAAAGCTCTTGGATGAAGCCATACAGGCTGTGAAGGTCCAGTCATTCCAAATGAAGA
GATGCCTGGACAAAAACAAGCTTATGGATGCTCTAAAACATGCTTCTAATATGCTTGGTGAACTCCGGACTTCTATGTTATCACCAAAGAGTTACTATGA
ACTTTATATGGCCATTTCTGATGAACTGCACTACTTGGAGGTCTACCTGACAGATGAGTTTGCTAAAGGAAGGAAAGTGGCAGATCTCTACGAACTTGTA
CAGTATGCTGGAAACATTATCCCAAGGCTTTACCTTTTGATCACAGTTGGAGTTGTATATGTCAAGTCATTTCCTCAGTCCAGGAAGGATATTTTGAAAG
ATTTGGTAGAAATGTGCCGTGGTGTGCAACATCCCTTGAGGGGTCTGTTTCTTCGAAATTACCTTCTTCAGTGTACCAGAAATATCTTACCTGATGAAGG
AGAGCCAACAGATGAAGAAACAACTGGTGACATCAGTGATTCCATGGATTTTGTACTGCTCAACTTTGCAGAAATGAACAAGCTCTGGGTGCGAATGCAG
CATCAGGGACATAGCCGAGATAGAGAAAAAAGAGAACGAGAAAGACAAGAACTGAGAATTTTAGTGGGAACAAATTTGGTGCGCCTCAGTCAGTTGGAAG
GTGTAAATGTGGAACGTTACAAACAGATGGTTTTGACTGGCATATTGGAGCAAGTTGTAAACTGTAGGGATGCTTTGGCTCAAGAATATCTCATGGAGTG
TATTATTCAGGTTTTCCCTGATGAATTTCACCTCCAGACTTTGAATCCTTTTCTTCGGGCCTGTGCTGAGTTACACCAGAATGTAAATGTGAAGAACATA
ATCATTGCTTTAATTGATAGATTAGCTTTATTTGCTCACCGTGAAGATGGACCTGGAATCCCAGCGGATATTAAACTTTTTGATATATTTTCACAGCAGG
TGGCTACAGTGATACAGTCTAGACAAGACATGCCTTCAGAGGATGTTGTATCTTTACAAGTCTCTCTGATTAATCTTGCCATGAAATGTTACCCTGATCG
TGTGGACTATGTTGATAAAGTTCTAGAAACAACAGTGGAGATATTCAATAAGCTCAACCTTGAACATATTGCTACCAGTAGTGCAGTTTCAAAGGAACTC
ACCAGACTTTTGAAAATACCAGTTGACACTTACAACAATATTTTAACAGTCTTGAAATTAAAACATTTTCACCCACTCTTTGAGTACTTTGACTACGAGT
CCAGAAAGAGCATGAGTTGTTATGTGCTTAGTAATGTTCTGGATTATAACACAGAAATTGTCTCTCAAGACCAGGTGGATTCCATAATGAATTTGGTATC
CACGTTGATTCAAGATCAGCCAGATCAACCTGTAGAAGACCCTGATCCAGAAGATTTTGCTGATGAGCAGAGCCTTGTGGGCCGCTTCATTCATCTGCTG
CGCTCTGAGGACCCTGACCAGCAGTACTTGATTTTGAACACAGCACGAAAACATTTTGGAGCTGGTGGAAATCAGCGGATTCGCTTCACACTGCCACCTT
TGGTATTTGCAGCTTACCAGCTGGCTTTTCGATATAAAGAGAATTCTAAAGTGGATGACAAATGGGAAAAGAAATGCCAGAAGATTTTTTCATTTGCCCA
CCAGACTATCAGTGCTTTGATCAAAGCAGAGCTGGCAGAATTGCCCTTAAGACTTTTTCTTCAAGGAGCACTAGCTGCTGGGGAAATTGGTTTTGAAAAT
CATGAGACAGTCGCATATGAATTCATGTCCCAGGCATTTTCTCTGTATGAAGATGAAATCAGCGATTCCAAAGCACAGCTAGCTGCCATCACCTTGATCA
TTGGCACTTTTGAAAGGATGAAGTGCTTCAGTGAAGAGAATCATGAACCTCTGAGGACTCAGTGTGCCCTTGCTGCATCCAAACTTCTAAAGAAACCTGA
TCAGGGCCGAGCTGTGAGCACCTGTGCACATCTCTTCTGGTCTGGCAGAAACACGGACAAAAATGGGGAGGAGCTTCACGGAGGCAAGAGGGTAATGGAG
TGCCTAAAAAAAGCTCTAAAAATAGCAAATCAGTGCATGGACCCCTCTCTACAAGTGCAGCTTTTTATAGAAATTCTGAACAGATATATCTATTTTTATG
AAAAGGAAAATGATGCGGTAACAATTCAGGTTTTAAACCAGCTTATCCAAAAGATTCGAGAAGACCTCCCGAATCTTGAATCCAGTGAAGAAACAGAGCA
GATTAACAAACATTTTCATAACACACTGGAGCATTTGCGCTTGCGGCGGGAATCACCAGAATCCGAGGGGCCAATTTATGAAGGTCTCATCCTTTAAGCG
GCCGC

Amino Acid Sequence

MYPYDVPDYAGSMPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKG
RKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFA
EMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQMVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAE
LHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHI
ATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQ
SLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGA
LAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGE
ELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEG
PIYEGLIL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU56175
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee