Pink1 (tribolium)

• Plasmid: pPIC9K Pink1 150-end 6HIS (opt) (tribolium) D359A E527A K528A Delta I261-L270 del linker 2
• Parent Plasmid: pPIC9K
• Expressed: alpha factor signal sequence Pink1 150-end 6HIS D359A E527A K528A Delta I261-L270 (tribolium) del linker 2
• Species: Tribolium castaneum

Sequence of Insert

GAATTCTCCAGATTTGAGAGTAATCCGATTACCTTGAACGACTTGTCATTAGGCAAGCCAATTGCCAAAGGAACTAACGGTGTCGTATATTCAGCTAAAG
TCAAAGACGATGAAACCGATGATAACAAGTATCCCTTTGCTCTTAAGATGATGTTCAACTACGACATTCAGTCCAATAGCATGGAGATCTTGAAGGCCAT
GTACAGAGAAACTGTGCCAGCTAGAATGTATTACAGTAATCACGATTTGAACAATTGGGAGATAGAGTTGGCTAACAGAAGGAAACACCTTCCACCGCAT
CCAAACATAGTAGCCATTTTCTCCGTGTTTACCGACTTGTATCCAGCTGCTTTACCACCTAGGTTGCATCCTGAAGGAGAAGGACGAAATATGTCCTTGT
TCCTATTGATGAAGCGTTATGACTGCAATCTTCAAAGCTTCCTTTCTACGGCACCTTCAACAAGAACAAGTCTGTTGTTATTGGCACAACTGCTGGAAGG
AGTCGCACATATGACTGCTCACGGTATTGCTCATAGAGACTTGAAGTCGGACAATTTGTTGCTTGATACATCTGAACCTGAAAGTCCAATTCTGGTGATT
TCTGCTTTTGGCTGTTGTTTGGCAGATAAAACCAATGGGTTATCCTTGCCCTATACCTCGTACGAAATGGATAAAGGTGGGAACACAGCCTTAATGGCAC
CAGAGATTATCTGCCAAAAGCCAGGTACCTTTTCCGTCCTTAATTACTCAAAAGCTGACTTATGGGCTGTTGGTGCCATTGCCTATGAGATCTTCAATTG
TCACAATCCATTTTACGGACCTTCTAGATTGAAGAACTTCAACTACAAAGAGGGAGATCTGCCCAAGCTACCTGATGAGGTTCCTACAGTTATCCAGGCA
TTAGTAGCAAACTTACTAAAGCGTAATCCAAACAAACGTTTGGATCCTGAAGTTGCCGCTAATGTTTGTCAACTGTTTCTGTGGGCACCTTCTACTTGGC
TGAAACCTGGTTTGAAGGTTCCGACTTCTGGTGAAATCCTACAATGGCTGCTTTCTCTAACTACTAAGGTTTTGTGCGAAGGCAAGATCAACAACAAATC
TTTCGGTGCTGCCTTTACGCGAAATTGGAGAAGAACTTATCCCGAGTACTTATTGATTTCAAGCTTTCTATGTAGGGCTAAATTGGCTAACGTTAGAAAC
GCTTTGCATTGGATACAGGAAAATCTTCCAGAGCTTGATCATCACCATCATCACCACTAAGCGGCCGC

Amino Acid Sequence

MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEASRFESNPITLN
DLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFALKMMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLPPHPNIVAIFSVFTDL
YPAALPPRLHPEGEGRNMSLFLLMKRYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLLDTSEPESPILVISAFGCCLADKTNG
LSLPYTSYEMDKGGNTALMAPEIICQKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDLPKLPDEVPTVIQALVANLLKRNPNKR
LDPEVAANVCQLFLWAPSTWLKPGLKVPTSGEILQWLLSLTTKVLCEGKINNKSFGAAFTRNWRRTYPEYLLISSFLCRAKLANVRNALHWIQENLPELD
HHHHHH*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU56098
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee