PINK1 (Tribolium)

• Plasmid: pPIC9K Pink1 150-end tribolium 6HIS (opt) (tribolium) S205D D359A E527A K528A Delta I261-L270
• Parent Plasmid: pPIC9K
• Expressed: alpha factor secretion signal Pink1 150-end tribolium 6HIS (opt) (tribolium) S205D D359A E527A K528A Delta I261-L270
• Tag: 6His
• Species: Tribolium castaneum

Sequence of Insert

GAATTCTCAAGATTTGAGTCAAATCCAATTACCCTGAATGATCTGTCTCTAGGCAAACCCATCGCCAAAGGGACAAATGGCGTTGTATACTCAGCAAAAG
TTAAGGATGACGAAACCGACGACAATAAGTATCCATTCGCCTTGAAGATGATGTTTAACTACGACATTCAGGATAACTCCATGGAAATCTTAAAGGCCAT
GTATAGGGAAACTGTCCCAGCAAGAATGTACTATAGCAATCACGACTTAAACAACTGGGAGATTGAATTGGCTAATCGTCGAAAGCATTTACCACCTCAT
CCTAACATAGTTGCAATCTTCAGTGTGTTTACCGATCTATATCCTGCTGCTTTGCCACCTAGACTTCACCCAGAAGGTGAAGGTAGGAACATGTCCTTGT
TTCTGTTGATGAAACGTTATGATTGCAATCTTCAGAGTTTTCTATCTACTGCTCCTTCGACACGAACTTCCTTGTTGTTATTGGCTCAATTGCTGGAAGG
AGTTGCTCACATGACTGCACATGGTATTGCCCACAGAGACTTGAAGTCTGACAATCTGTTGTTGGATACGTCTGAACCTGAGTCACCGATTCTTGTCATC
TCCGCTTTTGGATGTTGTTTGGCTGATAAGACAAACGGTTTGTCTTTGCCCTATACTAGCTACGAAATGGACAAAGGTGGAAATACCGCTCTTATGGCTC
CGGAGATTATCTGCCAAAAGCCAGGCACTTTCTCTGTGTTGAACTACTCCAAAGCAGATTTGTGGGCTGTTGGTGCCATAGCATACGAGATTTTCAACTG
TCATAATCCTTTCTACGGACCAAGTAGACTTAAGAACTTCAACTACAAGGAAGGTGATCTGCCTAAATTACCAGATGAGGTACCCACTGTCATTCAAGCC
TTAGTTGCCAATCTTCTGAAAAGAAACCCTAACAAGCGTTTAGATCCAGAAGTTGCTGCTAATGTATGCCAATTGTTTCTTTGGGCACCAAGTACCTGGC
TGAAACCTGGTCTTAAAGTGCCAACATCTGGAGAGATATTGCAGTGGTTACTTTCACTGACGACTAAAGTCTTGTGTGAAGGGAAGATCAACAATAAGTC
TTTTGGAGCTGCTTTTACCAGAAATTGGAGAAGAACATATCCCGAGTATCTATTGATTAGCTCGTTCTTGTGTAGAGCCAAATTGGCTAATGTTAGGAAC
GCACTACACTGGATTCAAGAGAACTTACCGGAGCTAGATCACCATCATCATCATCACTAAGCGGCCGC

Amino Acid Sequence

MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEAYVEFSRFESNP
ITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFALKMMFNYDIQDNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLPPHPNIVAIFSV
FTDLYPAALPPRLHPEGEGRNMSLFLLMKRYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLLDTSEPESPILVISAFGCCLAD
KTNGLSLPYTSYEMDKGGNTALMAPEIICQKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDLPKLPDEVPTVIQALVANLLKRN
PNKRLDPEVAANVCQLFLWAPSTWLKPGLKVPTSGEILQWLLSLTTKVLCEGKINNKSFGAAFTRNWRRTYPEYLLISSFLCRAKLANVRNALHWIQENL
PELDHHHHHH*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU56034
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee